Centella Asiatica Increased the Body Length Through the Modulation of Antioxidant in Rotenone-Induced Zebrafish Larvae

Abstract

Centella asiatica (CA) is herbal medicine that used as traditional medicine including ayurvedic theraphy since hundreds years ago. This herb containa of pentacyclic triterpenoids such as asiaticoside, madecassoside, Asiatic acid and brahmoside that proved had anti-oxidant and anti-inflammatory properties. This research aims to know the effect of ethanolic extract of CA extract against the length of rotenone-induced zebrafish larvae through the free radicals.mechanism. This research used zebrafish larvae until 6 dpf that consists of 5 groups (controls, rotenon 12.5 ppb on 2 hpf-3 dpf, and group treatment given rotenone 12.5 ppb 2 hpf-3 dpf and 5 µg/mL extract with long exposure to start 2 hpf to 4, 5 and 6 dpf respectively). The body length measured on 3-6 dpf using software Image Raster v 3.0 from optilab v 2.0. Malondialdehyde (MDA), superoxide Dismutase (SOD), catalase were measured by ELISA on 6 dpf. The results showed rotenon can inhibit the growth of length > 2 standard deviation (SD) and CA extract may increased ithe body in 6 dpf which correction value was 99.6%. CA extract significantly decreased the levels of MDA, and increased the level of SOD and catalase (p=0.000). Ethanol extract of Centella asiatica may increase in length through the modulation of oxidative stress.

The effect of Centella asiatica to the vascular endothelial growth factor and vascular endothelial growth factor receptor-2 on the rotenone induced zebrafish larvae (Danio rerio) stunting model

“…It makes the angiogenesis running normally that impacted to the escalation of body length [13]. The administration of Centella asiatica has proven in the enhancement of rotenone induced zebrafish body length through the elevation of BDNF, BAX, Hsp60 and SOD also decrease MDA [30][31]45].…”

Cinnamomum burmannii EXTRACT AMELIORATES HIGH GLUCOSE-INDUCED BRAIN APOPTOSIS IN ZEBRAFISH EMBRYOS THROUGH INHIBITION OF PROCASPASE-9 : IN SILICO AND IN VIVO STUDY

“…The ingredients of EM used with 10 times concentration is 0,815 gr MgSO4, 0.25 gr CaCl2, 0,15 gr KCl and 5 gr NaCl, diluted in 500 mL of aquadest. 23 Cinnamomum burmannii extraction Cinnamon bark quills (Materia Medica, East Java, Indonesia) were cleansed and pulverized. Cinnamomum burmannii extraction using ethanol 96% as solvent was carried out in accordance with previous research.…”

Hydroalcoholic extract of Centella asiatica ameliorate on 3-nitropropionic acid induced Huntington's like symptoms in adult zebrafish

“…Study revealed the cognitive enhancing and antioxidant property of HA-CA against streptozotocin (STZ) induced cognitive impairments and oxidative stress in rat model of Alzheimer disease (33). Evidenced showed that HA-CA increase body length in rotenone induced zebrafish larvae via the modulation of its antioxidant potential (34). Similarly, Janine et al, reported that HA-CA decrease ROS production, increase resistance to osmotic stress, increase life span, and heat stress due to its antioxidant potential in Chaenorhabditis elegans wild-type and knockout mutant (35).…”