Central and Peripheral Endocannabinoids and Cognate Acylethanolamides in Humans: Association with Race, Adiposity, and Energy Expenditure

Jumpertz, Reiner; Guijarro, Ana; Pratley, Richard E.; Piomelli, Daniele; Krakoff, Jonathan

doi:10.1210/jc.2010-2028

Cited by 49 publications

(45 citation statements)

References 20 publications

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“…As expected, [8][9][10] obese subjects had increased basal plasma AEA and 2-AG levels, which were positively correlated with body mass index and waist circumference, and also with insulin levels in the case of AEA (Figure 1). Just before the meal, AEA significantly increased in both normal-weight and obese individuals (Figure 2a).…”

Section: Resultssupporting

confidence: 74%

“…6,7 Elevated circulating AEA and 2-AG levels have been described in obese humans and, depending on the study, directly correlated with different measures of adiposity, including waist circumference, body fat percentage and body mass index. [8][9][10] However, endocannabinoids function in the context of human food intake regulation has remained elusive, and it is currently unknown whether they may participate in the pathophysiology of human obesity. Indeed, there are no information available about possible dynamic changes in plasma endocannabinoids before and after the consumption of a meal and on the possible alteration of such changes in obesity.…”

Section: Introductionmentioning

confidence: 99%

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Simultaneous postprandial deregulation of the orexigenic endocannabinoid anandamide and the anorexigenic peptide YY in obesity

et al. 2011

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Background:The endocannabinoid system is a potential pharmacotherapy target for obesity. However, the role of this system in human food intake regulation is currently unknown. Methods: To test whether circulating endocannabinoids might functionally respond to food intake and verify whether these orexigenic signals are deregulated in obesity alongside with anorexigenic ones, we measured plasma anandamide (AEA), 2-arachidonoylglycerol (2-AG) and peptide YY (PYY) changes in response to a meal in 12 normal-weight and 12 non-diabetic, insulin-resistant obese individuals. Results: Both normal-weight and obese subjects had a significant preprandial AEA peak. Postprandially, AEA levels significantly decreased in normal-weight, whereas no significant changes were observed in obese subjects. Similarly, PYY levels significantly increased in normal-weight subjects only. No meal-related changes were found for 2-AG. Postprandial AEA and PYY changes inversely correlated with waist circumference, and independently explained 20.7 and 21.3% of waist variance. Multiple regression analysis showed that postprandial AEA and PYY changes explained 34% of waist variance, with 8.2% of the variance commonly explained. Conclusion: These findings suggest that AEA might be a physiological meal initiator in humans and furthermore show that postprandially AEA and PYY are concomitantly deregulated in obesity.

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Section: Resultssupporting

confidence: 74%

Section: Introductionmentioning

confidence: 99%

Simultaneous postprandial deregulation of the orexigenic endocannabinoid anandamide and the anorexigenic peptide YY in obesity

et al. 2011

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“…We measured AEA, 2-AG, ten annotated NAEs, and eight putatively annotated NAEs, whereas previously only four endocannabinoids had been measured in CSF (22,23). Requiring only 200 l of CSF per sample, this sensitive method has LODs reaching picomolar-femtomolar levels, while maintaining good linearity and precision.…”

Section: Resultsmentioning

confidence: 99%

“…Although some analytical methods for profiling endocannabinoids and related NAEs in human plasma have been reported (25,27), methods for CSF are limited to AEA, 2-AG, PEA, and OEA (22,23). To achieve a highthroughput and sensitive method for detection and quantification of these compounds, two important parameters were optimized.…”

Section: Methods Developmentmentioning

confidence: 99%

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Quantitative profiling of endocannabinoids and related N-acylethanolamines in human CSF using nano LC-MS/MS

Kantae

Ogino

Noga

et al. 2017

Journal of Lipid Research

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The endocannabinoid system has emerged as a crucial regulator of synaptic communication in the CNS (1-3). Dysregulation of this system is implicated in various neurological and psychiatric disorders, such as neuroinflammation, stroke, brain trauma, anxiety, and depression (4-8). The endocannabinoid system consists of endogenous lipid messengers (endocannabinoids) that activate two distinct cannabinoid (CB) (CB 1 and CB 2 ) receptors and the en-

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Lipocalin‐Type Prostaglandin D Synthase Is a Novel Phytocannabinoid‐Binding Protein

Elmes

Volpe

d’Oelsnitz

et al. 2018

Lipids

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Lipocalin-type prostaglandin D synthase (L-PGDS; EC:5.3.99.2) is an enzyme with dual functional roles as a prostaglandin D -synthesizing enzyme and as an extracellular transporter for diverse lipophilic compounds in the cerebrospinal fluid (CSF). Transport of hydrophobic endocannabinoids is mediated by serum albumin in the blood and intracellularly by the fatty acid binding proteins, but no analogous transport mechanism has yet been described in CSF. L-PGDS has been reported to promiscuously bind a wide variety of lipophilic ligands and is among the most abundant proteins found in the CSF. Here, we examine the binding of several classes of endogenous and synthetic ligands to L-PGDS. Endocannabinoids exhibited low affinity toward L-PGDS, while cannabinoid metabolites and synthetic cannabinoids displayed higher affinities for L-PGDS. These results indicate that L-PGDS is unlikely to function as a carrier for endocannabinoids in the CSF, but it may bind and transport a subset of cannabinoids.

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Central and Peripheral Endocannabinoids and Cognate Acylethanolamides in Humans: Association with Race, Adiposity, and Energy Expenditure

Abstract: These data indicate a central alteration of the endocannabinoid system in American Indians and furthermore show that AEs in both compartments play an important but distinct role in human energy balance regulation.

Cited by 49 publications

References 20 publications

Simultaneous postprandial deregulation of the orexigenic endocannabinoid anandamide and the anorexigenic peptide YY in obesity

Simultaneous postprandial deregulation of the orexigenic endocannabinoid anandamide and the anorexigenic peptide YY in obesity

Quantitative profiling of endocannabinoids and related N-acylethanolamines in human CSF using nano LC-MS/MS

Lipocalin‐Type Prostaglandin D Synthase Is a Novel Phytocannabinoid‐Binding Protein

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