Central vasopressin administration failed to influence anxiety behavior after pinealectomy in rats

Appenrodt, E.; Schwarzberg, H

doi:10.1016/s0031-9384(99)00237-1

Cited by 19 publications

(9 citation statements)

References 34 publications

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“…On the day of the experiment, saline alone (250 nl per side), AVP (Sigma V9879, 1 ng in 250 nl per side of NaCl 0.9%, (Appenrodt and Schwarzberg, 2000) or V1a antagonist (d(CH 2 ) 5 1, Tyr(Me) 2, Arg 8 )-Vasopressin trifluoroacetate salt, Manning compound; 10 ng in 250 nl per side), depending the experimental groups, were injected bilaterally via an injection cannula (0.20 mm outer diameter, C315I, Plastics One), which protruded 1 mm beyond the end of the guide cannula, over a period of 5 min (Pérez de la Mora et al, 2006), using two CMA/Microdialysis pumps (CMA/Microdialysis, Stockholm, Sweden). The cannulae were kept in place for 1 min after the injection to prevent backflow and to allow for diffusion.…”

Section: Methodsmentioning

confidence: 99%

Hypothalamic Vasopressinergic Projections Innervate Central Amygdala GABAergic Neurons: Implications for Anxiety and Stress Coping

Hernández

Mora

et al. 2016

Front. Neural Circuits

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The arginine-vasopressin (AVP)-containing hypothalamic magnocellular neurosecretory neurons (VPMNNs) are known for their role in hydro-electrolytic balance control via their projections to the neurohypophysis. Recently, projections from these same neurons to hippocampus, habenula and other brain regions in which vasopressin infusion modulates contingent social and emotionally-affected behaviors, have been reported. Here, we present evidence that VPMNN collaterals also project to the amygdaloid complex, and establish synaptic connections with neurons in central amygdala (CeA). The density of AVP innervation in amygdala was substantially increased in adult rats that had experienced neonatal maternal separation (MS), consistent with our previous observations that MS enhances VPMNN number in the paraventricular (PVN) and supraoptic (SON) nuclei of the hypothalamus. In the CeA, V1a AVP receptor mRNA was only observed in GABAergic neurons, demonstrated by complete co-localization of V1a transcripts in neurons expressing Gad1 and Gad2 transcripts in CeA using the RNAscope method. V1b and V2 receptor mRNAs were not detected, using the same method. Water-deprivation (WD) for 24 h, which increased the metabolic activity of VPMNNs, also increased anxiety-like behavior measured using the elevated plus maze (EPM) test, and this effect was mimicked by bilateral microinfusion of AVP into the CeA. Anxious behavior induced by either WD or AVP infusion was reversed by CeA infusion of V1a antagonist. VPMNNs are thus a newly discovered source of CeA inhibitory circuit modulation, through which both early-life and adult stress coping signals are conveyed from the hypothalamus to the amygdala.

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Section: Methodsmentioning

confidence: 99%

Hypothalamic Vasopressinergic Projections Innervate Central Amygdala GABAergic Neurons: Implications for Anxiety and Stress Coping

Hernández

Mora

et al. 2016

Front. Neural Circuits

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“…Consistent with this evidence, another research has also shown that pinealectomy did not have a significant role on the acquisition and extinction of the active avoidance behaviour (Appenrodt and Schwarzberg 2003). In a similar vein, other studies that focused on some behavioural measures such as anxiety behaviour (Appenrodt and Schwarzberg 2000), passive avoidance learning (Appenrodt and Schwarzberg 1999), open-field exploratory activity (Kova´cs et al 1974) and social recognition (Appenrodt et al in the Morris water maze are represented. Right striated bar represents melatonin injection (Mel.…”

Section: Discussionmentioning

confidence: 55%

“…lesion on habenula) did impair cognitive performance (Lecourtier et al 2005). A great number of studies have consistently demonstrated that pinealectomy did not have a significant role on the acquisition and extinction of the active avoidance behaviour (Appenrodt and Schwarzberg 2003), anxiety behaviour (Appenrodt and Schwarzberg 2000), passive avoidance learning (Appenrodt and Schwarzberg 1999), open-field exploratory activity (Kova´cs et al 1974) and social recognition (Appenrodt et al 2002). On the other hand, whether or not pinealectomy decreases the learning capacity on the Morris water maze is still not known, and no published research has been reported about it so far.…”

Section: Introductionmentioning

confidence: 99%

The effects of pinealectomy, melatonin injections and implants on the spatial memory performance of male Wistar rats

Karakaş

Coşkun

Kaya

2011

Biological Rhythm Research

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Melatonin is the main hormone of the pineal gland that informs the body about the environmental light and darkness regimen and is considered to regulate many important physiological and behavioural activities. In the present study, we examined the effects of pinealectomy, constant-release melatonin implants and timed melatonin injections on spatial memory in male Wistar rats by using Morris water maze. Pinealectomy, melatonin injection and melatonin implantation were performed on groups, including controls for all groups. After a week of the pinealectomy and melatonin implantation surgeries, we applied the injections and made the training trials throughout 4 days. At the fifth day, experiments were performed and recorded. Spatial memory performance of the rats was impaired by the pinealectomy and melatonin injections, since they elongated the latency and shortened the time passed in the correct quadrant. Melatonin implantation did not affect the spatial memory performance of the rats. The latency and the time passed in correct quadrant were not statistically different from their controls. The results suggest that while the removal of the pineal gland and exogenous administration of melatonin via injections are causing impairment, constantrelease melatonin administration via implantation does not affect the spatial memory in Wistar albino rats.

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“…For instance, while the pinealectomy itself did not have a detrimental effect on cognitive performance in rats, the interaction of it with the other lesion (i.,e, lesion on habenula) impaired such performance (Lecourtier et al, 2005). Many studies have shown that pinealectomy did not have a significant effect on the acquisition and extinction of the active avoidance behavior (Appenrodt and Schwarzberg, 2003), anxiety behavior (Appenrodt and Schwarzberg, 2000), passive avoidance learning (Appenrodt and Schwarzberg, 1999), open field exploratory activity (Kovács et al, 1974), and social recognition (Appenrodt et al, 2002).…”

Section: Pinealectomymentioning

confidence: 99%

Intraamygdalar Melatonin Administration and Pinealectomy Affect Anxiety Like Behavior and Spatial Memory

Karakaş¹,

Coşkun²

2012

Neuroendocrinology and Behavior

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Central vasopressin administration failed to influence anxiety behavior after pinealectomy in rats

Cited by 19 publications

References 34 publications

Hypothalamic Vasopressinergic Projections Innervate Central Amygdala GABAergic Neurons: Implications for Anxiety and Stress Coping

Hypothalamic Vasopressinergic Projections Innervate Central Amygdala GABAergic Neurons: Implications for Anxiety and Stress Coping

The effects of pinealectomy, melatonin injections and implants on the spatial memory performance of male Wistar rats

Intraamygdalar Melatonin Administration and Pinealectomy Affect Anxiety Like Behavior and Spatial Memory

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