Centriolar satellites: Busy orbits around the centrosome

Bärenz, Felix; Mayilo, Dmytro; Gruß, Oliver J.

doi:10.1016/j.ejcb.2011.07.007

Cited by 81 publications

(83 citation statements)

References 75 publications

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“…The punctate localization of C2cd3 around the centrosome was reminiscent of centriolar satellites, dynamic electron-dense granular structures that surround the centrosome and ciliary basal body in interphase but are dispersed in mitosis (16). We observed that in mitosis, C2cd3 was present as two fine dots inside each spindle pole instead of as punctae around it, consistent with the cell cycle-dependent dynamics of centriolar satellites ( Fig.…”

Section: Resultssupporting

confidence: 65%

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C2cd3 is critical for centriolar distal appendage assembly and ciliary vesicle docking in mammals

Zeng

Ning

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

133

158

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The primary cilium plays critical roles in vertebrate development and physiology, but the mechanisms underlying its biogenesis remain poorly understood. We investigated the molecular function of C2 calcium-dependent domain containing 3 (C2cd3), an essential regulator of primary cilium biogenesis. We show that C2cd3 is localized to the centriolar satellites in a microtubule-and Pcm1-dependent manner; however, C2cd3 is dispensable for centriolar satellite integrity. C2cd3 is also localized to the distal ends of both mother and daughter centrioles and is required for the recruitment of five centriolar distal appendage proteins: Sclt1, Ccdc41, Cep89, Fbf1, and Cep164. Furthermore, loss of C2cd3 results in failure in the recruitment of Ttbk2 to the ciliary basal body as well as the removal of Cp110 from the ciliary basal body, two critical steps in initiating ciliogenesis. C2cd3 is also required for recruiting the intraflagellar transport proteins Ift88 and Ift52 to the mother centriole. Consistent with a role in distal appendage assembly, C2cd3 is essential for ciliary vesicle docking to the mother centriole. Our results suggest that C2cd3 regulates cilium biogenesis by promoting the assembly of centriolar distal appendages critical for docking ciliary vesicles and recruiting other essential ciliogenic proteins.

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Section: Resultssupporting

confidence: 65%

“…Centriolar satellites are electron-dense particles around the centrosome and basal body (16). Many centriolar satellite components have been identified, including Pcm1, the BBSome, a multiprotein complex comprising seven Bardet-Biedl syndromerelated proteins, as well as Cep290 and Ofd1 (17)(18)(19)(20).…”

mentioning

confidence: 99%

C2cd3 is critical for centriolar distal appendage assembly and ciliary vesicle docking in mammals

Zeng

Ning

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

133

158

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“…Importantly, centrioles nucleate the formation of the primary cilium, which functions as a hub for developmentally important signaling pathways including Hedgehog, Wnt and platelet-derived growth factor receptor-α pathways (Berbari et al, 2009;Kim and Dynlacht, 2013). In addition to centrioles and PCM, vertebrate cells have an array of 70-100 nm membrane-less structures that localize and move around the centrosome and cilium complex in a microtubule-and molecular motor-dependent manner, termed "centriolar satellites' (Barenz et al, 2011;Tollenaere et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

The centriolar satellite protein CCDC66 interacts with CEP290 and functions in cilium formation and trafficking

Conkar

Culfa

Odabasi

et al. 2017

Journal of Cell Science

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Centriolar satellites are membrane-less structures that localize and move around the centrosome and cilium complex in a microtubuledependent manner. They play important roles in centrosome-and cilium-related processes, including protein trafficking to the centrosome and cilium complex, and ciliogenesis, and they are implicated in ciliopathies. Despite the important regulatory roles of centriolar satellites in the assembly and function of the centrosome and cilium complex, the molecular mechanisms of their functions remain poorly understood. To dissect the mechanism for their regulatory roles during ciliogenesis, we performed an analysis to determine the proteins that localize in close proximity to the satellite protein CEP72, among which was the retinal degeneration gene product CCDC66. We identified CCDC66 as a microtubuleassociated protein that dynamically localizes to the centrosome, centriolar satellites and the primary cilium throughout the cell cycle. Like the BBSome component BBS4, CCDC66 distributes between satellites and the primary cilium during ciliogenesis. CCDC66 has extensive proximity interactions with centrosome and centriolar satellite proteins, and co-immunoprecipitation experiments revealed interactions between CCDC66, CEP290 and PCM1. Ciliogenesis, ciliary recruitment of BBS4 and centriolar satellite organization are impaired in cells depleted for CCDC66. Taken together, our findings identify CCDC66 as a targeting factor for centrosome and cilium proteins.

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“…Perfect chromosome alignment leads to spindle assembly checkpoint (SAC) silence and activates APC/C which degrades the seperase inhibitor, securin. As anaphase onset, activated separase dissolves the cohesion between sister-chromatids which can move toward the spindle pole by the pulling force of the spindle [2].…”

Section: Introductionmentioning

confidence: 99%