Centriolin, a centriole-appendage protein, regulates peripheral spindle migration and asymmetric division in mouse meiotic oocytes

Abstract

Unlike somatic cells mitosis, germ cell meiosis consists of 2 consecutive rounds of division that segregate homologous chromosomes and sister chromatids, respectively. The meiotic oocyte is characterized by an absence of centrioles and asymmetric division. Centriolin is a relatively novel centriolar protein that functions in mitotic cell cycle progression and cytokinesis. Here, we explored the function of centriolin in meiosis and showed that it is localized to meiotic spindles and concentrated at the spindle poles and midbody during oocyte meiotic maturation. Unexpectedly, knockdown of centriolin in oocytes with either siRNA or Morpholino micro-injection, did not affect meiotic spindle organization, cell cycle progression, or cytokinesis (as indicated by polar body emission), but led to a failure of peripheral meiotic spindle migration, large polar body emission, and 2-cell like oocytes. These data suggest that, unlike in mitotic cells, the centriolar protein centriolin does not regulate cytokinesis, but plays an important role in regulating asymmetric division of meiotic oocytes.

Potential Regulatory Role of Human-Carboxylesterase-1 Glycosylation in Liver Cancer Cell Growth

“…ZC3H12A, a tumor suppressor, induces apoptosis and cell death, , whereas CEP152 and CNTRL are centrosomal proteins involved in centriole duplication and mitotic spindle migration during the cell cycle . Dysregulation of these two proteins has been shown to induce p53-dependent apoptosis . In addition, the expression levels of the proliferation markers, K i-67 and TOP2A , , were downregulated in the CES1 -OE cells, suggesting that they may be regulated by CES1.…”

“…51 Dysregulation of these two proteins has been shown to induce p53-dependent apoptosis. 52 In addition, the expression levels of the proliferation markers, Ki-67 and TOP2A, 53,54 were downregulated in the CES1-OE cells, suggesting that they may be regulated by CES1. Therefore, we predicted that CES1 may be involved in the induction of Hep3B cell apoptosis.…”

Neural Precursor Cells Expanded Inside the 3D Micro-Scaffold Nichoid Present Different Non-Coding RNAs Profiles and Transcript Isoforms Expression: Possible Epigenetic Modulation by 3D Growth

“…Among the switched isoforms with functional consequences identified, Cntrl emerged as the most significant one. Interestingly, this gene is involved in centrosome maturation and microtubules organization and has been identified as key regulator in asymmetrical division [ 78 ], suggesting how splicing events may play an important role in altering gene expression related to cytoskeletal re-arrangement.…”

Oncogenic FGFR Fusions Produce Centrosome and Cilia Defects by Ectopic Signaling

“…CEP110 is a structural protein of the centrosome [351,352], for which it requires a 170aa region in the C-terminus that is retained in the CEP110-FGFR1 fusion (Figure 1) [247]. The centrosome localization of the fusion may, therefore, interfere with centrosome maturation, likely due to combination of the steric effects of the fusion and its ectopic kinase activity, which in turn produces centrosomal and spindle abnormalities and drives the oncogenesis [351,353,354].…”