Ceratopogonidae (Diptera) Reared from Cacti, with a Review of the Copiosus Group of Culicoides

Wirth, Willis W.; Hubert, A. A.

doi:10.1093/aesa/53.5.639

Cited by 11 publications

(29 citation statements)

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“…Culicoides specimens were selected for genetic characterisation following preliminary identification using an SMZ-140 stereomicroscope (Motic, Barcelona, Spain) and the keys of Boorman [38], Gangopadhyay & Das Gupta [22] and Wirth & Hubert [18], and the descriptions of Majumdar et al [39] and Nandi & Mazumdah [40, 41]. …”

Section: Methodsmentioning

confidence: 99%

“…Following collection, specimens of Culicoides were identified morphologically under a stereomicroscope using keys [18, 63] and comparison to reference specimens from the local area produced as part of the phylogenetic analysis conducted within this study. Specimens were sexed and females were further separated based on their abdominal pigmentation status (unpigmented/ nulliparous, blood-fed, gravid, or pigmented/ parous) [64, 65].…”

Section: Methodsmentioning

confidence: 99%

“…The Culicoides fauna of the Oriental region has been the focus of an authoritative taxonomic review based on morphology [18]. Wirth & Hubert’s review [18], however, did not extend to a comprehensive review of the Culicoides fauna of the Indian subcontinent and the Culicoides fauna of India has only been subject to sporadic morphological studies, e.g.…”

Section: Introductionmentioning

confidence: 99%

“…Wirth & Hubert’s review [18], however, did not extend to a comprehensive review of the Culicoides fauna of the Indian subcontinent and the Culicoides fauna of India has only been subject to sporadic morphological studies, e.g. Das Gupta [19, 20].…”

Section: Introductionmentioning

confidence: 99%

“…in Australia is the result of field studies demonstrating that the primary BTV vector species in this region, C. brevitarsis , has a greater sensitivity to this wavelength than to other colours including UV [31]. As both C. imicola and C. brevitarsis occur in India [18], it is important to define prior to the initiation of wide-scale surveillance project which light wavelength is most effective for surveillance of these and other Culicoides spp. in southern India.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

DNA barcoding and surveillance sampling strategies for Culicoides biting midges (Diptera: Ceratopogonidae) in southern India

et al. 2016

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BackgroundCulicoides spp. biting midges transmit bluetongue virus (BTV), the aetiological agent of bluetongue (BT), an economically important disease of ruminants. In southern India, hyperendemic outbreaks of BT exert high cost to subsistence farmers in the region, impacting on sheep production. Effective Culicoides spp. monitoring methods coupled with accurate species identification can accelerate responses for minimising BT outbreaks. Here, we assessed the utility of sampling methods and DNA barcoding for detection and identification of Culicoides spp. in southern India, in order to provide an informed basis for future monitoring of their populations in the region.MethodsCulicoides spp. collected from Tamil Nadu and Karnataka were used to construct a framework for future morphological identification in surveillance, based on sequence comparison of the DNA barcode region of the mitochondrial cytochrome c oxidase I (COI) gene and achieving quality standards defined by the Barcode of Life initiative. Pairwise catches of Culicoides spp. were compared in diversity and abundance between green (570 nm) and ultraviolet (UV) (390 nm) light emitting diode (LED) suction traps at a single site in Chennai, Tamil Nadu over 20 nights of sampling in November 2013.ResultsDNA barcode sequences of Culicoides spp. were mostly congruent both with existing DNA barcode data from other countries and with morphological identification of major vector species. However, sequence differences symptomatic of cryptic species diversity were present in some groups which require further investigation. While the diversity of species collected by the UV LED Center for Disease Control (CDC) trap did not significantly vary from that collected by the green LED CDC trap, the UV CDC significantly outperformed the green LED CDC trap with regard to the number of Culicoides individuals collected.ConclusionsMorphological identification of the majority of potential vector species of Culicoides spp. samples within southern India appears relatively robust; however, potential cryptic species diversity was present in some groups requiring further investigation. The UV LED CDC trap is recommended for surveillance of Culicoides in southern India.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-016-1722-z) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

DNA barcoding and surveillance sampling strategies for Culicoides biting midges (Diptera: Ceratopogonidae) in southern India

et al. 2016

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Introduction to the Taxonomy and General Biology of Diptera (Insecta) Involved in the Transmission of Avian Haemosporida

Ibáñez-Bernal

Rivera-García

Abella-Medrano

2020

Avian Malaria and Related Parasites in the Tropics

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Morphological and Molecular Identification ofCulicoides(Diptera: Ceratopogonidae) Species of the Southern California Desert

Zhang

Phillips²,

Gerry

2022

Journal of Medical Entomology

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Culicoides Latreille (Diptera: Ceratopogonidae) biting midges are vectors of important animal pathogens including bluetongue virus (BTV) and epizootic hemorrhagic disease virus (EHDV). While some Culicoides species present in the southern California desert are implicated in the transmission of these viruses to ruminant animals, these species have not been extensively studied due in part to the challenge of identifying Culicoides to species and to the lack of published gene sequences for these species to support their molecular identification. In this study, Culicoides were captured using suction traps baited with either carbon dioxide or UV light from transitional habitat between the southern California peninsular mountain ranges and the Colorado desert of southeastern California. Captured midges were initially identified using traditional morphological methods, with species identification subsequently confirmed by sequence analysis of COI and 28S rDNA genes. Phylogenetic analyses support that some Culicoides subgenera are not monophyletic. Two recognized species (C. sitiens Wirth and Hubert and C. bakeri Vargas) shared the same COI and 28S sequences. An additional cryptic species may be present within C. sitiens. Two additional recognized species (C. cacticola Wirth and Hubert and C. torridus Wirth and Hubert) may be conspecific or cryptic to each other. In total, 19 Culicoides species (or species aggregate) were collected in this study, with genetic sequences published for the first time for 16 of them. Published genetic sequences will support future research on these species, including studies on the ecology and habits of their immature stages which are often tedious to identify using morphology.

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Ceratopogonidae (Diptera) Reared from Cacti, with a Review of the Copiosus Group of Culicoides

Cited by 11 publications

References 0 publications

DNA barcoding and surveillance sampling strategies for Culicoides biting midges (Diptera: Ceratopogonidae) in southern India

DNA barcoding and surveillance sampling strategies for Culicoides biting midges (Diptera: Ceratopogonidae) in southern India

Introduction to the Taxonomy and General Biology of Diptera (Insecta) Involved in the Transmission of Avian Haemosporida

Morphological and Molecular Identification ofCulicoides(Diptera: Ceratopogonidae) Species of the Southern California Desert

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