Cereal seed storage protein synthesis: fundamental processes for recombinant protein production in cereal grains

Kawakatsu, Taiji; Takaiwa, Fumio

doi:10.1111/j.1467-7652.2010.00559.x

Cited by 124 publications

(77 citation statements)

References 173 publications

(336 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although both PSV proteins and PB proteins are synthesized as secretory proteins on rough ER, PSV proteins are transported into PSVs via Golgi or directly as PAC vesicles, whereas PB proteins aggregate to form PBs in the ER lumen (Kawakatsu and Takaiwa, 2010). Cereal grains contain either PSVs or ER-derived PBs as the deposition sites of SSPs (Shewry and Halford, 2002).…”

Section: Effects Of Psv and Pb Protein Reduction On Pb And Psv Formatmentioning

confidence: 99%

Reducing Rice Seed Storage Protein Accumulation Leads to Changes in Nutrient Quality and Storage Organelle Formation

et al. 2010

Self Cite

View full text Add to dashboard Cite

Rice (Oryza sativa) seed storage proteins (SSPs) are synthesized and deposited in storage organelles in the endosperm during seed maturation as a nitrogen source for germinating seedlings. We have generated glutelin, globulin, and prolamin knockdown lines and have examined their effects on seed quality. A reduction of one or a few SSP(s) was compensated for by increases in other SSPs at both the mRNA and protein levels. Especially, reduction of glutelins or sulfur-rich 10-kD prolamin levels was preferentially compensated by sulfur-poor or other sulfur-rich prolamins, respectively, indicating that sulfurcontaining amino acids are involved in regulating SSP composition. Furthermore, a reduction in the levels of 13-kD prolamin resulted in enhancement of the total lysine content by 56% when compared with the wild type. This observation can be mainly accounted for by the increase in lysine-rich proteins. Although reducing the level of glutelins slightly decreased protein storage vacuoles (PSVs), the simultaneous reduction of glutelin and globulin levels altered the inner structure of PSVs, implicating globulin in framing PSV formation. Knock down of 13-kD prolamins not only reduced the size of endoplasmic reticulumderived protein bodies (PBs) but also altered the rugged peripheral structure. In contrast, PBs became slightly smaller or unchanged by severe suppression of 10-or 16-kD prolamins, respectively, indicating that individual prolamins have distinct functions in the formation of PBs. Extreme increases or decreases in sulfur-poor prolamins resulted in the production of small PBs, suggesting that the ratio of individual prolamins is crucial for proper aggregation and folding of prolamins.

show abstract

Section: Effects Of Psv and Pb Protein Reduction On Pb And Psv Formatmentioning

confidence: 99%

Reducing Rice Seed Storage Protein Accumulation Leads to Changes in Nutrient Quality and Storage Organelle Formation

et al. 2010

Self Cite

View full text Add to dashboard Cite

show abstract

“…5,9 Seed specific expression of seed storage proteins (SSPs) is determined by the combinatory interaction between several cis-regulatory elements, and transcription factors recognizing the individual cognate cis-element have been also characterized. 10,11 The accumulation levels of foreign recombinant proteins fundamentally depends on the yield potentiality of the target tissues (cells) of the host crop. 1,12 Furthermore, the yield of recombinant protein products is determined at both the transcriptional and post-transcriptional level.…”

mentioning

confidence: 99%

Increasing the production yield of recombinant protein in transgenic seeds by expanding the deposition space within the intracellular compartment

Takaiwa

2013

Bioengineered

Self Cite

View full text Add to dashboard Cite

show abstract

“…Production in cereal crop seeds has already been demonstrated to increase the yield of recombinant proteins based on the established expression system using a strong seed-specific promoter, codon optimization, and a specific host reducing storage proteins and depositing into protein bodies [25][26][27]. Furthermore, protein productions in rice seeds have the additional benefit of high biomass yield with a minimal risk of outcrossing.…”

Section: New Effective Means For Rice Growth In Plant Factoriesmentioning

confidence: 99%

Feasibility Study of Rice Growth in Plant Factories

Yamori¹,

Zhang²

2014

J Rice Res

View full text Add to dashboard Cite

Global food production will need to increase more than 50% before 2050 to satisfy the food demands of an increasing human population. To solve this food crisis, an increase in rice production will be necessary as rice is especially vital for the nutrition of much of the population in many countries. One solution to the future food shortage worldwide would be to cultivate crops in places where plants to do typically grow (e.g., desert and Antarctic). The technology for "plant factories", a new facility to grow plants under a controlled environment that would enable high yield and high quality production year-round, is progressing. Through the computer-based, automated control of environmental conditions for optimal rice growth, the external environmental conditions would have little effect on the plants growing inside the plant factory. In the near future, the plant factory could play an important role in areas where field productions are difficult or impossible. Moreover, since producing biopharmaceuticals in plants through genetic engineering technologies has many economic and qualitative benefits, the trial to produce useful materials in transgenic plants is examined in the plant factory. Transgenic plants might therefore expand the usefulness of plant factories. In this review article, feasibility of rice growth in plant factories is summarized.

show abstract

Cereal seed storage protein synthesis: fundamental processes for recombinant protein production in cereal grains

Cited by 124 publications

References 173 publications

Reducing Rice Seed Storage Protein Accumulation Leads to Changes in Nutrient Quality and Storage Organelle Formation

Reducing Rice Seed Storage Protein Accumulation Leads to Changes in Nutrient Quality and Storage Organelle Formation

Increasing the production yield of recombinant protein in transgenic seeds by expanding the deposition space within the intracellular compartment

Feasibility Study of Rice Growth in Plant Factories

Contact Info

Product

Resources

About