Cerium-containing mesoporous bioactive glasses: Material characterization, in vitro bioactivity, biocompatibility and cytotoxicity evaluation

Atkinson, Irina; Anghel, Elena Maria; Petrescu, Simona; Seciu, Ana‐Maria; Stefan, Laura; Mocioiu, Oana Cătălina; Predoană, Luminița; Voicescu, Mariana; Şomăcescu, Simona; Culiță, Daniela C.; Zaharescu, Maria

doi:10.1016/j.micromeso.2018.09.029

Cited by 49 publications

(58 citation statements)

References 45 publications

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“…A similar effect was also seen in a previous study, where 0.8 mol% Ag 2 O-doped 80SiO 2 -15CaO-5P 2 O 5 MSG [11] had 528 m 2 /g surface area compared to 351 m 2 /g of undoped-MSG [11,28]. In most studies, cerium incorporation into MBGs was found to decrease the surface area [34,35]. In contrast to these, the results from the present study have shown that the surface area of Ce-MSG was increased.…”

Section: Discussionsupporting

confidence: 85%

A Structural Comparison of Ordered and Non-Ordered Ion Doped Silicate Bioactive Glasses

et al. 2020

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One of the key benefits of sol-gel-derived glasses is the presence of a mesoporous structure and the resulting increase in surface area. This enhancement in textural properties has a significant effect on the physicochemical properties of the materials. In this context the aim of this study was to investigate how sol-gel synthesis parameters can influence the textural and structural properties of mesoporous silicate glasses. We report the synthesis and characterization of metal ion doped sol-gel derived glasses with different dopants in the presence or absence of a surfactant (Pluronic P123) used as structure-directing templating agent. Characterization was done by several methods. Using a structure directing agent led to larger surface areas and highly ordered mesoporous structures. The chemical structure of the non-ordered glasses was modified to a larger extent than the one of the ordered glasses due to increased incorporation of dopant ions into the glass network. The results will help to further understand how the properties of sol-gel glasses can be controlled by incorporation of metal dopants, in conjunction with control over the textural properties, and will be important to optimize the properties of sol-gel glasses for specific applications, e.g., drug delivery, bone regeneration, wound healing, and antibacterial materials.

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Section: Discussionsupporting

confidence: 85%

A Structural Comparison of Ordered and Non-Ordered Ion Doped Silicate Bioactive Glasses

et al. 2020

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“…The potential in vivo applications of these materials needs their testing for cytocompatibility [32,33], especially after the results obtained on cerium-doped bioglasses in terms of enzymatic-like CAT and SOD activities [25,27]. Therefore, in this paper three glasses based on BG and modified with increasing amount of doping CeO 2 (1.2, 3.6 and 5.3 mol% of CeO 2 , called BG_1.2, BG_3.6 and BG_5.3, respectively) have been synthesized and tested in cell culture medium for their cytocompatibility with murine long bone osteocyte-like (MLO-Y4) and mouse embryonic fibroblast (NIH/3T3) cell lines.…”

Section: Introductionmentioning

confidence: 99%

Cytocompatibility of Potential Bioactive Cerium-Doped Glasses based on 45S5

et al. 2019

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The cytocompatibility of potential bioactive cerium-containing (Ce3+/Ce4+) glasses is here investigated by preparing three different glasses with increasing amount of doping CeO2 (1.2, 3.6 and 5.3 mol% of CeO2, called BG_1.2, BG_3.6 and BG_5.3, respectively) based on 45S5 Bioglass® (called BG). These materials were characterized by Environmental Scanning Electron Microscopy (ESEM) and infrared spectroscopy (FTIR) after performing bioactivity tests in Dulbecco’s Modified Eagle Medium (DMEM) solution, and the ions released in solution were determined by Inductively Coupled Plasma Mass Spectrometry (ICP-MS) and Optical Emission Spectrometry (ICP-OES). The data obtained clearly show that the glass surfaces of BG, BG_1.2 and BG_3.6 were covered by hydroxyapatite (HA), while BG_5.3 favored the formation of a cerium phosphate crystal phase. The cytotoxicity tests were performed using both murine long bone osteocyte-like (MLO-Y4) and mouse embryonic fibroblast (NIH/3T3) cell lines. The cerium-containing bioactive glasses show an increment in cell viability with respect to BG, and at long times, no cell aggregation and deformation were observed. The proliferation of NIH/3T3 cells increased with the cerium content in the glasses; in particular, BG_3.6 and BG_5.3 showed a higher proliferation of cells than the negative control. These results highlight and enforce the proposal of cerium-doped bioactive glasses as a new class of biomaterials for hard-tissue applications.

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“…After soaking in simulated body fluid (Dulbecco’ Modified Essential Medium, DMEM solution) these glasses, particularly the 1.2 and 3.6 mol% of CeO 2 ones, are bioactive in terms of the formation of apatite (hydroxyapatite, HA). As preliminary cytocompatibility tests are required for in vivo applications [37,38], we treated them in a cell culture medium with a murine long bone osteocyte-like cell line (MLO-Y4); cerium-containing glasses show an increment in cell viability with respect to 45S5 Bioglass ® ; furthermore, no cell aggregation and deformation were observed at long incubation times (72 h). In particular, the glasses with 1.2 and 3.6 mol% of CeO 2 are likely to be suited and are promising scaffolds for hard-tissue applications.…”

Section: Introductionmentioning

confidence: 99%

P2O5-Free Cerium Containing Glasses: Bioactivity and Cytocompatibility Evaluation

2019

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(1) Background: valuation of the bioactivity and cytocompatibility of P2O5-free and CeO2 doped glasses. (2) Methods: all glasses are based on the Kokubo (K) composition and prepared by a melting method. Doped glassed, K1.2, K3.6 and K5.3 contain 1.2, 3.6, and 5.3 mol% of CeO2. Bioactivity and cytotoxicity tests were carried out in simulated body fluid (SBF) solution and murine osteocyte (MLO-Y4) cell lines, respectively. Leaching of ions concentration in SBF was determined by inductively coupled plasma mass spectrometry (ICP-MS) and optical emission spectrometry (ICP-OES). The surface of the glasses were characterized by scanning electron microscopy (SEM) and X-ray diffraction (XRD) techniques. (3) Results: P2O5-free cerium doped glasses are proactive according to European directives. Cerium increases durability and retards, but does not inhibit, (Ca10(PO4)6(OH)2, HA) formation at higher cerium amounts (K3.6 and K5.3); however, cell proliferation increases with the amount of cerium especially evident for K5.3. (4) Conclusions: These results enforce the use of P2O5-free cerium doped bioactive glasses as a new class of biomaterials.

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Cerium-containing mesoporous bioactive glasses: Material characterization, in vitro bioactivity, biocompatibility and cytotoxicity evaluation

Cited by 49 publications

References 45 publications

A Structural Comparison of Ordered and Non-Ordered Ion Doped Silicate Bioactive Glasses

A Structural Comparison of Ordered and Non-Ordered Ion Doped Silicate Bioactive Glasses

Cytocompatibility of Potential Bioactive Cerium-Doped Glasses based on 45S5

P2O5-Free Cerium Containing Glasses: Bioactivity and Cytocompatibility Evaluation

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