Certified dOFM sampling devices provide access to target tissue in pharmaceutical trials

Bodenlenz, Manfred; Dragatin, Christian; Hoefferer, C.; Birngruber, Thomas; Priedl, J.; Feichtner, Franz; Schaller, Roland; Aigner, Birgit; Korsatko, Stefan; Pieber, Thomas R.; Sinner, Frank

doi:10.1515/bmt-2013-4133

Cited by 2 publications

(1 citation statement)

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“…In this study, we established an innovative platform for cultivation and online monitoring of 3D cell and tissue cultures. Integrating OFM, a proven probe-based in vivo sampling method [21,22] that is used to evaluate the pharmacokinetics and pharmacodynamics of drugs directly in skin [23], adipose [24], and brain [25] tissue in vivo, in a bioreactor opens new horizons for its applicability in in vitro models and tissues. The further implementation of sensors to the OFM in the bioreactor complements the monitoring platform enabling the sampling and online monitoring of the interstitial fluid.…”

Section: Discussionmentioning

confidence: 99%

Innovative Platform for the Advanced Online Monitoring of Three-Dimensional Cells and Tissue Cultures

Kreß

Schaller‐Ammann

Feiel

et al. 2022

Cells

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The use of 3D cell cultures has gained increasing importance in medical and pharmaceutical research. However, the analysis of the culture medium is hardly representative for the culture conditions within a 3D model which hinders the standardization of 3D cultures and translation of results. Therefore, we developed a modular monitoring platform combining a perfusion bioreactor with an integrated minimally invasive sampling system and implemented sensors that enables the online monitoring of culture parameters and medium compounds within 3D cultures. As a proof-of-concept, primary cells as well as cell lines were cultured on a collagen or gelatin methacryloyl (GelMA) hydrogel matrix, while monitoring relevant culture parameters and analytes. Comparing the interstitial fluid of the 3D models versus the corresponding culture medium, we found considerable differences in the concentrations of several analytes. These results clearly demonstrate that analyses of the culture medium only are not relevant for the development of standardized 3D culture processes. The presented bioreactor with an integrated sampling and sensor platform opens new horizons for the development, optimization, and standardization of 3D cultures. Furthermore, this technology holds the potential to reduce animal studies and improve the transferability of pharmaceutical in vitro studies by gaining more relevant results, bridging the gap towards clinical translation.

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