Cervical Cytology as a Screening Method

Höffken, H.; Soost, H.-J.

doi:10.1007/978-3-642-68185-1_2

Cited by 3 publications

(4 citation statements)

References 32 publications

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“…Cervical cancer screening and diagnosis is combined liquid-based cytology stained Papanicolaou stain (also known as Pap smear) and HPV testing using DNA/RNA PCR-based methods ( 12 , 13 , 61 ). Papanicolaou carried out the first prospective studies of the vaginal cycle by working with guinea pigs, and in 1943, jointly with Traut, he outlined detailed studies of cycle-dependent epithelial changes in the vaginal epithelium of the human female ( 62 ). Epithelial cells are collected from the external surface of the cervix and lower part of the cervical canal using a cervical sampling brush or spatula, processed into a thin layer on a glass microscope slide, stained with Papanicolaou stain, and evaluated by a cytopathologist using a microscope ( 62 ).…”

Section: Cervical Cancer Screening and Diagnostic Tools For Hpv Detec...mentioning

confidence: 99%

“…Papanicolaou carried out the first prospective studies of the vaginal cycle by working with guinea pigs, and in 1943, jointly with Traut, he outlined detailed studies of cycle-dependent epithelial changes in the vaginal epithelium of the human female ( 62 ). Epithelial cells are collected from the external surface of the cervix and lower part of the cervical canal using a cervical sampling brush or spatula, processed into a thin layer on a glass microscope slide, stained with Papanicolaou stain, and evaluated by a cytopathologist using a microscope ( 62 ). The cytopathologist evaluates the sample by comparing the histologic structure to the normal squamous epithelium from the vagina and ectocervix ( 62 ).…”

Section: Cervical Cancer Screening and Diagnostic Tools For Hpv Detec...mentioning

confidence: 99%

“…Epithelial cells are collected from the external surface of the cervix and lower part of the cervical canal using a cervical sampling brush or spatula, processed into a thin layer on a glass microscope slide, stained with Papanicolaou stain, and evaluated by a cytopathologist using a microscope ( 62 ). The cytopathologist evaluates the sample by comparing the histologic structure to the normal squamous epithelium from the vagina and ectocervix ( 62 ). Höffken et al.…”

Section: Cervical Cancer Screening and Diagnostic Tools For Hpv Detec...mentioning

confidence: 99%

“…Höffken et al. ( 62 ) summarized the histology and cytology of a normal squamous epithelium from the vagina and ectocervix as shown in Table 3 .…”

Section: Cervical Cancer Screening and Diagnostic Tools For Hpv Detec...mentioning

confidence: 99%

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Molecular Detection Methods in HPV-Related Cancers

2022

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Human papillomavirus (HPV) is responsible for most cervical cancers and some head and neck cancers, including oropharyngeal squamous cell carcinoma and sinonasal carcinoma. Cervical cancer is commonly diagnosed by liquid-based cytology, followed by HPV testing using commercially available DNA polymerase chain reaction (PCR), p16 immunohistochemistry (IHC), or DNA/RNA in situ hybridization. HPV in head and neck cancers is commonly diagnosed by p16 IHC or by RT-qPCR of HPV-16 E6 and E7 oncoproteins. Droplet digital PCR has been reported as an ultrasensitive and highly precise method of nucleic acid quantification for biomarker analysis and has been used to detect oncogenic HPV in oropharyngeal and cervical cancers.

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