Cervicovaginal fluid cytokines as predictive markers of preterm birth in symptomatic women

Park, Sunwha; You, Young Ah; Yun, Hayoung; Choi, Suk‐Joo; Hwang, Han-Sung; Choi, Sae-Kyung; Lee, Seung Mi; Kim, Young Ju

doi:10.5468/ogs.19131

Cited by 35 publications

(30 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To date, clinicians and researchers have made great efforts to improve the identification of women at risk for PTB before its occurrence, as well as to develop therapeutics for its prevention. However, the early identification of PTB and therapy to mitigate its risk remain controversial [ 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

Elevated methylation of the vault RNA2-1 promoter in maternal blood is associated with preterm birth

et al. 2021

Self Cite

View full text Add to dashboard Cite

Background Preterm birth, defined as parturition before 37 completed weeks of gestation, is associated with an increased risk of neonatal complications and death, as well as poor health and disease later in life. Epigenetics could contribute to the mechanism underlying preterm birth. Results Genome-wide DNA methylation analysis of whole blood cells from 10 women (5 term and 5 preterm deliveries) was performed using an Illumina Infinium HumanMethylation450 BeadChips array. We identified 1,581 differentially methylated CpG sites in promoter regions between term and preterm birth. Although the differences were not significant after correcting for multiple tests, seven CpGs on the genomically imprinted vault RNA2-1 (VTRNA2-1; also known as non-coding RNA, nc886 or miR-886) showed the largest differences (range: 26–39 %). Pyrosequencing verification was performed with blood samples from pregnant women recruited additionally (39 term and 43 preterm deliveries). In total, 28 (34.1 %) samples showed hypomethylation of the VTRNA2-1 promoter (< 13 % methylation), while 54 (65.9 %) samples showed elevated methylation levels between 30 and 60 %. Elevated methylation of VTRNA2-1 promoter was associated with an increased risk of preterm birth after adjusting for maternal age, season of delivery, parity and white blood cell count. The mRNA expression of VTRNA2-1 was 0.51-fold lower in women with preterm deliveries (n = 20) compared with women with term deliveries (n = 20). Conclusions VTRNA2-1 is a noncoding transcript to environmentally responsive epialleles. Our results suggest that elevated methylation of the VTRNA2-1 promoter may result in increased risk of PTB caused by the pro-inflammatory cytokines. Further studies are needed to confirm the association of VTRNA2-1 methylation with preterm birth in a large population, and to elucidate the underlying mechanism.

show abstract

Section: Introductionmentioning

confidence: 99%

Elevated methylation of the vault RNA2-1 promoter in maternal blood is associated with preterm birth

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Poor conditions of the medical, social, economic and particularly differences in ethnicity are prime factors of PTB [ 6 ]. Several measures have been introduced for the monitoring of PTB, like the examination of the previous history of PTB, a short cervix (< 25 mm), vaginal pH, pro-inflammatory cytokines (IL-6) in cervicovaginal fluid (CVF), or fetal fibronectin (FFN) analysis [ 7 , 8 ]. These measures merely offer superficial information, and are thus inadequate for the confirmation of the PTB-causing agent.…”

Section: Introductionmentioning

confidence: 99%

Identification of Potential Biomarkers in the Cervicovaginal Fluid by Metabolic Profiling for Preterm Birth

et al. 2020

Self Cite

View full text Add to dashboard Cite

During pregnancy, dysbiosis in the vaginal microbiota directly affects the metabolic profiles, which might impact preterm birth (PTB). In this study, we performed cervicovaginal fluid (CVF) metabolic profiling using nuclear magnetic resonance (NMR) spectroscopy and identified the metabolic markers for predicting PTB. In this nested case-control study, 43 South Korean pregnant women with PTB (n = 22), and term birth (TB; n = 21) were enrolled with their demographic profiles, and CVF samples were collected by vaginal swabs. The PTB group had two subgroups based on post-CVF sampling birth: PTB less than (PTB < 7 d) and more than 7 days (PTB ≥ 7 d). We observed significant differences in the gestational age at birth (GAB), cervical length (CL), and neonatal birth weight among the groups. The principal component analysis (PCA), and partial least square discriminant analysis (PLS-DA) scatter plot showed the separation between the PTB < 7 d group, and the TB group. Out of 28 identified metabolites, acetone, ethanol, ethylene glycol, formate, glycolate, isopropanol, methanol, and trimethylamine N-oxide (TMAO) were significantly increased in the PTB group compared with the TB group. The ROC curve analysis revealed that the acetone, ethylene glycol, formate, glycolate, isopropanol, methanol, and TMAO had the best predictive values for PTB. Additionally, the correlation analysis of these metabolites showed a strong negative correlation with GAB and CL. These metabolites could be beneficial markers for the clinical application of PTB prediction.

show abstract

“…Bead-based multiplex arrays for flow cytometry have become a standard technology for the fast and high-throughput analysis of cytokine concentrations in biological samples, such as blood or cell culture supernatants (57,58).…”

Section: Proteomics Approachesmentioning

confidence: 99%

Phenotyping of Adaptive Immune Responses in Inflammatory Diseases

et al. 2020

View full text Add to dashboard Cite

Immunophenotyping on the molecular and cellular level is a central aspect for characterization of patients with inflammatory diseases, both to better understand disease etiopathogenesis and based on this to develop diagnostic and prognostic biomarkers which allow patient stratification and tailor-made treatment strategies. Technology-driven developments have considerably expanded the range of analysis tools. Especially the analysis of adaptive immune responses, often regarded as central though mostly poorly characterized disease drivers, is a major focus of personalized medicine. The identification of the disease-relevant antigens and characterization of corresponding antigen-specific lymphocytes in individual patients benefits significantly from recent developments in cytometry by sequencing and proteomics. The aim of this workshop was to identify the important developments for state-of-the-art immunophenotyping for clinical application and precision medicine. We focused here on recent key developments in analysis of antigen-specific lymphocytes, sequencing, and proteomics approaches, their relevance in precision medicine and the discussion of the major challenges and opportunities for the future.

show abstract

Cervicovaginal fluid cytokines as predictive markers of preterm birth in symptomatic women

Cited by 35 publications

References 43 publications

Elevated methylation of the vault RNA2-1 promoter in maternal blood is associated with preterm birth

Elevated methylation of the vault RNA2-1 promoter in maternal blood is associated with preterm birth

Identification of Potential Biomarkers in the Cervicovaginal Fluid by Metabolic Profiling for Preterm Birth

Phenotyping of Adaptive Immune Responses in Inflammatory Diseases

Contact Info

Product

Resources

About