Challenges and opportunities for engineered Escherichia coli as a pivotal chassis toward versatile tyrosine-derived chemicals production

Effendi, Sefli Sri Wahyu; Ng, I-Son

doi:10.1016/j.biotechadv.2023.108270

Cited by 5 publications

(5 citation statements)

References 162 publications

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“…In nature, only a few bacteria can naturally assimilate acetate. Among them, Escherichia coli , a model microorganism with the advantages of fast growth in cheap media and simple genetic manipulation protocols, has been widely used in the microbial fermentation of different valuable chemicals [ 10 , 11 ]. As a result, microbial production from acetate using E. coli as a chassis has become a promising research area.…”

Section: Introductionmentioning

confidence: 99%

Application of Acetate as a Substrate for the Production of Value-Added Chemicals in Escherichia coli

Gu,

Li,

Huang

et al. 2024

Microorganisms

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At present, the production of the majority of valuable chemicals is dependent on the microbial fermentation of carbohydrate substrates. However, direct competition is a potential problem for microbial feedstocks that are also used within the food/feed industries. The use of alternative carbon sources, such as acetate, has therefore become a research focus. As a common organic acid, acetate can be generated from lignocellulosic biomass and C1 gases, as well as being a major byproduct in microbial fermentation, especially in the presence of an excess carbon source. As a model microorganism, Escherichia coli has been widely applied in the production of valuable chemicals using different carbon sources. Recently, several valuable chemicals (e.g., succinic acid, itaconic acid, isobutanol, and mevalonic acid) have been investigated for synthesis in E. coli using acetate as the sole carbon source. In this review, we summarize the acetate metabolic pathway in E. coli and recent research into the microbial production of chemical compounds in E. coli using acetate as the carbon source. Although microbial synthetic pathways for different compounds have been developed in E. coli, the production titer and yield are insufficient for commercial applications. Finally, we discuss the development prospects and challenges of using acetate for microbial fermentation.

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Section: Introductionmentioning

confidence: 99%

Application of Acetate as a Substrate for the Production of Value-Added Chemicals in Escherichia coli

Gu,

Li,

Huang

et al. 2024

Microorganisms

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“…Among the numerous valuable aromatic compounds, polyphenols represent a substantial group derived from phenolic substances. In 2020, they reached a market value of $1.6 billion, and their compound annual growth rate (CAGR) is projected to be 5.2% from 2021 to 2030 [1]. Notably, certain compounds with anti-tumor and antibacterial properties, such as resveratrol and ferulic acid, achieved market sizes of 71.9 million and 67.8 million in 2020 and 2022, respectively.…”

Section: Introductionmentioning

confidence: 99%

“…Notably, certain compounds with anti-tumor and antibacterial properties, such as resveratrol and ferulic acid, achieved market sizes of 71.9 million and 67.8 million in 2020 and 2022, respectively. They are expected to reach 130 million by 2030 [1]. Additionally, compounds such as tyrosol [2], salidroside [3] and salvianic acid A have gained prominence in the fields of medicine and food health.…”

Section: Introductionmentioning

confidence: 99%

“…Among various microorganisms, E. coli is one of the most widely used hosts for microbial factories due to its rapid growth rate, unique characteristics and well-characterized centralized regulatory system [7]. 4-hydroxyphenylpyruvate (HPP) plays a pivotal role as a fundamental precursor in the biosynthesis of aromatic compounds, and the synthesis of numerous highvalue compounds can be achieved based on this premise [1,8] (Figure 1). Monitoring the amount of HPP in chassis cells can provide valuable support for the biosynthesis of these downstream compounds, and the development of biosensors for precursor compounds is beneficial for the synthesis of various downstream compounds [9].…”

Section: Introductionmentioning

confidence: 99%

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Directed Evolution of 4-Hydroxyphenylpyruvate Biosensors Based on a Dual Selection System

Du,

Liang,

et al. 2024

IJMS

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Biosensors based on allosteric transcription factors have been widely used in synthetic biology. In this study, we utilized the Acinetobacter ADP1 transcription factor PobR to develop a biosensor activating the PpobA promoter when bound to its natural ligand, 4-hydroxybenzoic acid (4HB). To screen for PobR mutants responsive to 4-hydroxyphenylpyruvate(HPP), we developed a dual selection system in E. coli. The positive selection of this system was used to enrich PobR mutants that identified the required ligands. The following negative selection eliminated or weakened PobR mutants that still responded to 4HB. Directed evolution of the PobR library resulted in a variant where PobRW177R was 5.1 times more reactive to 4-hydroxyphenylpyruvate than PobRWT. Overall, we developed an efficient dual selection system for directed evolution of biosensors.

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“…Some phenolic acids and monolignols can inhibit microbial growth, which is a limiting factor for the microbial synthesis of coniferyl alcohol, and screening and the expression of efflux proteins are key to aromatic synthesis . Heterologous expression of AtABCG29 in yeasts has been noted to result in increased tolerance to p -coumaryl alcohol .…”

Section: Introductionmentioning

confidence: 99%

Improving Production of Coniferyl Alcohol in Escherichia coli by Regulating the Multiple Cofactors and Efflux of Intermediates

Wang,

Chen,

et al. 2024

ACS Sustainable Chem. Eng.

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Challenges and opportunities for engineered Escherichia coli as a pivotal chassis toward versatile tyrosine-derived chemicals production

Cited by 5 publications

References 162 publications

Application of Acetate as a Substrate for the Production of Value-Added Chemicals in Escherichia coli

Application of Acetate as a Substrate for the Production of Value-Added Chemicals in Escherichia coli

Directed Evolution of 4-Hydroxyphenylpyruvate Biosensors Based on a Dual Selection System

Improving Production of Coniferyl Alcohol in Escherichia coli by Regulating the Multiple Cofactors and Efflux of Intermediates

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