Challenges attending upon studies on clavulanic acid production

Aim: Evaluate the chemopreventive potential of the extract from P. polymyxa RNC-D. Methods: Concentrations of P. polymyxa RNC-D extract were tested in HepG2/C3A cells to assess their genotoxic (comet assay), mutagenic (micronucleus test) and antigenotoxic potential (comet assay) in vitro. Results: 400 and 40 μg/ml concentrations induced DNA lesions, whereas the 4 μg/ml induced a desmutagenic effect. Complementary tests indicated that the extract minimized the formation of reactive oxygen species induced by methyl methanesulfonate and normalized the loss of membrane potential. The quantification of cytokines indicated that TNF-α was immunostimulated by the extract. However, when administered in conjunction with the methyl methanesulfonate, the extract blocked the TNF-α release. Conclusion: The fermentation broth from P. polymyxa RNC-D showed an antigenotoxic effect, and thus the potential to be used as chemopreventive compound.

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Challenges attending upon studies on clavulanic acid production

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Endophytic Microorganisms of the Tropical Savannah: A Promising Source of Bioactive Molecules

Endophytic Microorganisms of the Tropical Savannah: A Promising Source of Bioactive Molecules

Antigenotoxic Potential of the Fermentation Broth Produced by Paenibacillus Polymyxa RNC-D In Vitro

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