Challenges in Analyzing Bioactive Proanthocyanidins

Zeller, Wayne E.; Mueller-Harvey, I.

doi:10.1002/9781119545958.ch6

Cited by 2 publications

(1 citation statement)

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“…Other studies indicated that oligomeric (mDP 4–10) and polymeric (mDP > 10) proanthocyanidins can be found in the lingonberry matrix as well [ 2 , 32 ], thus suggesting unidentified larger polymeric molecules in our tested sample. Nevertheless, liquid chromatography coupled to photodiode-array or mass detection may be useful for authentication, but it is not suitable for accurate quantification since not all analytical standards are available and response factors for the individual polymers are unknown [ 33 , 34 ]. Quantification problems are usually overcome by the DMAC spectrophotometric assay, which is regarded as a simple, rapid, robust, and relatively specific technique for the evaluation of the total amount of proanthocyanidins [ 34 , 35 , 36 ].…”

Section: Discussionmentioning

confidence: 99%

Proanthocyanidins from Vaccinium vitis-idaea L. Leaves: Perspectives in Wound Healing and Designing for Topical Delivery

Vilkickytė

Žilius

Petrikaitė

et al. 2022

Plants

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The compositions and health-beneficial properties of lingonberry leaves (Vaccinium vitis-idaea L.) are well established; however, their proanthocyanidins are still heavily underutilized. Optimizing their delivery systems is key to enabling their wider applications. The present study investigates the phytochemical and ‘wound-healing’ properties of proanthocyanidin-rich fraction(s) (PRF) from lingonberry leaves as well as the development of optimal dermal film as a proanthocyanidin delivery system. The obtained PRF was subjected to HPLC-PDA and DMAC analyses to confirm the qualitative and quantitative profiles of different polymerization-degree proanthocyanidins. A ‘wound healing’ in vitro assay was performed to assess the ability of PRF to modulate the wound environment for better healing. Low concentrations of lingonberry proanthocyanidins were found to accelerate ‘wound‘ closures, while high levels inhibited human fibroblast migration. Fifteen dermal films containing PRF were prepared and evaluated based on their polymer (MC, HEC, PEG 400) compositions, and physical, mechanical, and biopharmaceutical properties using an experimental design. The composition containing 0.30 g of MC, 0.05 g of HEC, and 3.0 g of PEG 400 was selected as a promising formulation for PRF delivery and a potentially effective functional wound dressing material, supporting the need for further investigations.

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