Challenges in Cardiac Tissue Engineering

Vunjak-Novakovic, Gordana; Tandon, Nina; Godier, Anne; Maidhof, Robert; Marsano, Anna; Martens, Timothy P.; Radišić, Milica

doi:10.1089/ten.teb.2009.0352

Cited by 468 publications

(411 citation statements)

References 128 publications

(183 reference statements)

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“…Other organs in the body are also affected by the compromised blood supply. At some point in the progression of the disease, the heart reaches a stage where it is not able to keep up with the demands of the body and consequently, a heart attack occurs [4] .…”

Section: Myocardial Infarctionmentioning

confidence: 99%

“…Studies have focused on either delivering the cells in cell suspension or injecting cells along with a flexible biomaterial, such as a hydrogel, which would assume the shape of the defect and provide the cells a protective as well as proliferative environment while they are integrated into the host tissue [20].…”

Section: Cardiac Tissue Engineeringmentioning

confidence: 99%

“…Although, the former is the ideal hope, the current state of research hopes to achieve the latter. Implanting the construct on top of the infracted region would add a level of functionality to that region and either improve the overall heart function or prevent it from degrading any further [20].…”

Section: Engineered Heart Tissues (Ehts)mentioning

confidence: 99%

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Endothelial cells guided by immobilized gradients of vascular endothelial growth factor on porous collagen scaffolds

et al. 2011

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A key challenge in tissue engineering is overcoming cell death in the scaffold interior due to the limited diffusion of oxygen and nutrients therein. We hypothesized here that immobilizing a gradient of vascular endothelial growth factor would guide endothelial cells into the interior of the scaffold thereby enhancing angiogenesis. The protein was immobilized onto a collagen scaffold through carbodiimide chemistry by one of the three methods experimented: placing 5 µl of the solution at the center of the scaffold to create a ~2 ng/ml/mm gradient in a radial direction. D4T endothelial cells were observed to be guided by this VEGF-165 gradient deep into the center of the scaffold compared to both uniformly immobilized VEGF-165 and VEGFfree controls. We concluded that the VEGF-165 gradient scaffolds promoted the migration, and not proliferation, of cells deep into the scaffold. These gradient scaffolds provide the foundation for future in vivo tissue engineering studies.iii

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Section: Myocardial Infarctionmentioning

confidence: 99%

Section: Cardiac Tissue Engineeringmentioning

confidence: 99%

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Endothelial cells guided by immobilized gradients of vascular endothelial growth factor on porous collagen scaffolds

et al. 2011

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“…For example, several approaches for engineering cardiac cell "sheets" or "patches" that can be implanted onto hearts in vivo have been demonstrated and shown to mitigate damage induced by myocardial infarction and ischemia. [82][83][84][85] Approaches for engineering other types of vascularized tissue, such as bone, kidney, and pancreas, have also met with significant success. [86][87][88] Many others have cataloged the progress of tissue engineering and the advances in manufacturing technologies and novel materials that have rendered possible medical applications of biofabrication.…”

Section: Tissue Engineeringmentioning

confidence: 99%

Biomimicry, Biofabrication, and Biohybrid Systems: The Emergence and Evolution of Biological Design

Raman

Bashir

2017

Adv Healthcare Materials

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how the concept of bioinspiration, or imitating biological design and functionality in synthetic materials, created a new class of "smart" biomimetic materials. Then, we shall discuss how the continuing development of enabling manufacturing technologies, such as 3D printing and microfluidics, has established the separate subdiscipline of biofabrication for tissue engineering, or "building with biology." Finally, we shall investigate the convergence of these two fields into the emerging discipline of biohybrid design, the use of biological materials to power non-natural functional behaviors in synthetic machines. Throughout this report, we will revisit a single class of materials, hydrogels, as a case study of biological design in the context of each subfield, and discuss how the constraints, underlying principles, and end-use applications differ in each case. We will also discuss ethical considerations of biological design, with a special focus on forward engineering non-natural or hypernatural functional behaviors in biohybrid systems. We will conclude with recommendations for implementing biological design into educational curricula, ensuring effective and responsible practices for the next generation of engineers and scientists. Biomimicry and Bioinspired DesignA deeper understanding of the underlying design principles that govern biological systems has inspired the field of biomimicry. [1,2] Observing adaptive phenomena in nature, scientists and engineers have sought to extract the components of biological design responsible for this behavior and replicate the behavior in synthetic materials. Fundamentally, this involves understanding the building blocks or base units from which a biological material is built, the hierarchical assembly of these building blocks, and the interactions and interfaces between them.In this section, we will discuss strategies that engineers and scientists have employed to engineer bioinspired hierarchy, from bottom-up self-assembly and top-down engineered assembly. We will present several key demonstrations of stimulus-responsive hydrogels ranging from the micro-to the macroscale, and investigate novel demonstrations in biomimetic actuation and movement. We will conclude with the remaining challenges in the field of biomimicry and discuss the potential future impact of bioinspired design.The discipline of biological design has a relatively short history, but has undergone very rapid expansion and development over that time. This Progress Report outlines the evolution of this field from biomimicry to biofabrication to biohybrid systems' design, showcasing how each subfield incorporates bioinspired dynamic adaptation into engineered systems. Ethical implications of biological design are discussed, with an emphasis on establishing responsible practices for engineering non-natural or hypernatural functional behaviors in biohybrid systems. This report concludes with recommendations for implementing biological design into educational curricula, ensuring effective and responsible practice...

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“…Therefore myocardial tissue engineering approaches have to be developed considering both cell and GF requirements of the heart for successful cardiac recovery. In addition, functional integration between the graft and the host tissue, in both electromechanical and vascular terms, still remains a major challenge that must be considered when designing new cardiac tissue engineering approaches [154]. The establishment of welldefined protocols and the optimization of the synergies between the different cells and GFs are required before clinical applications can be attained.…”

Section: Challenges Aheadmentioning

confidence: 99%

Heart regeneration after myocardial infarction using synthetic biomaterials

Pascual-Gil

Garbayo

Díaz-Herráez

et al. 2015

Journal of Controlled Release

122

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Abstract:Myocardial infarction causes almost 7.3 million deaths each year worldwide. However, current treatments are more palliative than curative. Presently, cell and protein therapies are considered the most promising alternative treatments. Clinical trials performed until now have demonstrated that these therapies are limited by protein short half-life and by low transplanted cell survival rate, prompting the development of novel cell and protein delivery systems able to overcome such limitations. In this review we discuss the advances made in the last 10 years in the emerging field of cardiac repair using biomaterial-based delivery systems with focus on the progress made on preclinical in vivo studies. Then, we focus in cardiac tissue engineering approaches, and how the incorporation of both cells and proteins together into biomaterials has opened new horizons in the myocardial infarction treatment. Finally, the ongoing challenges and the perspectives for future work in cardiac tissue engineering will also be discussed.

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Challenges in Cardiac Tissue Engineering

Cited by 468 publications

References 128 publications

Endothelial cells guided by immobilized gradients of vascular endothelial growth factor on porous collagen scaffolds

Endothelial cells guided by immobilized gradients of vascular endothelial growth factor on porous collagen scaffolds

Biomimicry, Biofabrication, and Biohybrid Systems: The Emergence and Evolution of Biological Design

Heart regeneration after myocardial infarction using synthetic biomaterials

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