Challenges in diagnosing Zika—experiences from a reference laboratory in a non-endemic setting

Bossche, Dorien Van den; Míchiels, Johan; Cnops, Lieselotte; Foqué, Nikki; Meersman, Kathleen; Huits, Ralph; Ariën, Kevin K.; Esbroeck, Marjan Van

doi:10.1007/s10096-019-03472-8

Cited by 8 publications

(6 citation statements)

References 29 publications

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“…Sensitivities up till 68% were reported in panels limiting the number of days pso (Lustig et al 2017;Steinhagen et al 2016). The low sensitivity and short duration of positivity of the Euroimmun IgM ELISA is the reason why the IFA Arbovirus Fever Mosaic 2 was added to our diagnostic portfolio already soon after the start of the ZIKV outbreak and why IgM and IgG detection should be combined (Van den Bossche et al 2019). Sensitivity of the NS1 based NovaLisa® Zika IgM μ-capture ELISA (NovaTec Immundiagnostica GmbH, Dietzenbach, Germany) was 65% between 2 and 118 and 69.8% between 1 and 164 days pso (Basile et al 2018;Safronetz et al 2017).…”

Section: Discussionmentioning

confidence: 99%

Performance evaluation of the Diasorin LIAISON® XL Zika capture IgM CLIA test

Beken

Geyter

Esbroeck

2019

Diagnostic Microbiology and Infectious Disease

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Section: Discussionmentioning

confidence: 99%

Performance evaluation of the Diasorin LIAISON® XL Zika capture IgM CLIA test

Beken

Geyter

Esbroeck

2019

Diagnostic Microbiology and Infectious Disease

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“…Acute samples were obtained between day 1 to 5 AOS and three convalescent samples: early, between day 10 to 28 AOS; mid, between day 65-126 AOS; and late, day 142 to 203 AOS were also obtained. Immunoreactivity was further characterized by measuring neutralizing Abs (NAbs) against the four DENV serotypes, ZIKV, and CHIKV using in house whole virus neutralization tests ( 39 , 40 ), and against YFV using PRNT ( Table 1 ).…”

Section: Methodsmentioning

confidence: 99%

Dynamics of the Magnitude, Breadth and Depth of the Antibody Response at Epitope Level Following Dengue Infection

et al. 2021

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Dengue is a major public health problem in tropical and sub-tropical regions worldwide. Since the Zika epidemic and the increased co-circulation of other arboviruses, the serology-based diagnosis of dengue has become more problematic due to the high antigenic resemblance, especially among the flavivirus family. Therefore, a more comprehensive understanding of the diversity, specificity and temporal evolution of the antibody response following dengue infection is needed. In order to close this knowledge gap, we used a high-density peptide microarray of 9,072 linear peptides covering the entire proteome diversity of dengue, Zika, yellow fever and chikungunya viruses. The IgM and IgG antibody responses were measured against the designed microarray in symptomatic dengue infected individuals from an arbovirus endemic area in Peru and in overseas travelers returning to Belgium, as representatives of multiple-exposed and primary infections, respectively. Serum samples were collected longitudinally across four time points over the period of six months in Peru and over two time points in travelers. We show that epitopes eliciting the strongest flavivirus cross-reactive antibodies, in both primary and secondary infections were concentrated in the capsid, E, NS1, NS3 and NS5 proteins. The IgG antibody responses against NS1 and NS3 followed a rise-and-fall pattern, with peak titers between two to four weeks after onset of illness. The response to the E and NS5 proteins increased rapidly in the acute phase and was maintained at stable levels until at least 6 months after illness. A more scattered IgM antibody reactivity across the viral proteome was observed in the acute phase of the disease and that persisted through the 6-month window. The magnitude, breadth (i.e. number of unique epitopes targeted) and depth (i.e. number of epitope variants recognized) of the IgG response was higher in secondary infections compared to primary infections. For IgM antibodies, the magnitude of the response was higher in primary infected individuals whereas the breadth and depth of the response was lower in this group compared with the endemic subjects. Finally, through this arboviral proteome-wide epitope mapping, we were able to identify IgM and IgG dengue-specific epitopes which can be useful serological markers for dengue diagnosis and serostatus determination.

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“…Main serological tests for the diagnosis of ZIKV infection include ELISA for the detection of antibodies IgM and IgG against ZIKV [30]. Additionally, other serological methods such as immunofluorescence assays (IFA), immunoblots and (chemiluminescent) microsphere immunoassays (CMIA) are also being used [61,62]. Generally, a positive IgM indicates a recent infection, while a positive IgG result indicates a past flavivirus infection (presumptive positive infection of ZIKV or other flaviviruses) [63].…”

Section: Screening and Diagnosismentioning

confidence: 99%