Challenges in Healing Wound: Role of Complementary and Alternative Medicine

Monika, Prakash; Chandraprabha, M.N.; Rangarajan, Annapoorni; Waiker, P. Veena; Murthy, Kotamballi N. Chidambara

doi:10.3389/fnut.2021.791899

Cited by 134 publications

(89 citation statements)

References 84 publications

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“…There is an association between wound healing processes and oxidative stress-regulating activity, and one of the main factors that play a fundamental role in wound healing is oxidation; hence, antioxidant polyphenolic substances of plant origin may be a particularly important aspect ( Ustuner et al, 2019 ; Comino-Sanz et al, 2021 ; Monika et al, 2022 ). The presence of several polyphenolic compounds, mainly of a flavonoid nature, was revealed in the aerial parts of Jasione montana L. (Campanulaceae).…”

Section: Introductionmentioning

confidence: 99%

Wound Healing Properties of Jasione montana Extracts and Their Main Secondary Metabolites

et al. 2022

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The effects of different extracts obtained from Jasione montana L. (JM1–JM6) and their main metabolites on biological processes during wound healing were evaluated. The effect on wound closure in the scratch test was established, and collagen type I synthesis and anti-inflammatory effects were assessed by flow cytometry in a human dermal fibroblast model (PCS-201-012). Additionally, the antioxidant activity (DPPH and FRAP) and degree of inhibition of elastase participating in the proliferation processes of skin fibroblasts were determined in an in vitro model. The extracts and fractions were analyzed using high-performance liquid chromatography–photodiode array detection (HPLC–PDA) to quantitatively characterize their main polyphenolic compounds. The high antioxidant activity of the JM4–JM5 fractions correlated with the content of luteolin and its derivative 7-O-glucoside. Luteolin also showed the highest anti-elastase activity with an IC50 value of 39.93 ± 1.06 μg/mL, and its substantial content in the JM4 fraction presumably determines its activity (359.03 ± 1.65 μg/mL). At lower concentrations (<50 μg/mL) of all extracts, cell proliferation and migration were significantly stimulated after 24 h of treatment. The stimulation of cell migration was comparable with that of allantoin, which was used as a positive control. However, most of the tested extracts showed limited capacity to affect collagen type I biosynthesis. Moreover, the tested samples exhibited a complex effect on cytokine secretion, and the strongest anti-inflammatory activity through the moderation of IL-1β, IL-6 and IL-8 was observed for JM4 and luteolin. Based on the obtained results of the quantitative analysis, the anti-inflammatory activity of JM4 may be due to the high content of luteolin. In summary, extracts from J. montana, which is flavonoid-rich, promote the viability and accelerate the migration of fibroblasts as well as moderate oxidant and inflammatory processes and elastase activity. Hence, they may be potentially useful for topical therapeutic applications to stimulate the wound healing process.

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Section: Introductionmentioning

confidence: 99%

Wound Healing Properties of Jasione montana Extracts and Their Main Secondary Metabolites

et al. 2022

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“…The CCk-8 assay demonstrated that DGS significantly increased the viability of HUVECs, which established that DGS could promote the proliferation of HUVECs. The ability of DGS to promote the migration of HUVECs was then assessed using the wound healing assay [40], transwell assay [41], and tube formation assay [42]. The results showed that DGS significantly stimulated the migration of HU-VECs towards the scratch region and the lower transwell compartment and significantly stimulated the tube formation ability of HUVECs.…”

Section: Discussionmentioning

confidence: 99%

Investigation Driven by Network Pharmacology on Potential Components and Mechanism of DGS, a Natural Vasoprotective Combination, for the Phytotherapy of Coronary Artery Disease

Zhang

Liu²,

Zong³

et al. 2022

Molecules

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Phytotherapy offers obvious advantages in the intervention of Coronary Artery Disease (CAD), but it is difficult to clarify the working mechanisms of the medicinal materials it uses. DGS is a natural vasoprotective combination that was screened out in our previous research, yet its potential components and mechanisms are unknown. Therefore, in this study, HPLC-MS and network pharmacology were employed to identify the active components and key signaling pathways of DGS. Transgenic zebrafish and HUVECs cell assays were used to evaluate the effectiveness of DGS. A total of 37 potentially active compounds were identified that interacted with 112 potential targets of CAD. Furthermore, PI3K-Akt, MAPK, relaxin, VEGF, and other signal pathways were determined to be the most promising DGS-mediated pathways. NO kit, ELISA, and Western blot results showed that DGS significantly promoted NO and VEGFA secretion via the upregulation of VEGFR2 expression and the phosphorylation of Akt, Erk1/2, and eNOS to cause angiogenesis and vasodilation. The result of dynamics molecular docking indicated that Salvianolic acid C may be a key active component of DGS in the treatment of CAD. In conclusion, this study has shed light on the network molecular mechanism of DGS for the intervention of CAD using a network pharmacology-driven strategy for the first time to aid in the intervention of CAD.

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“…Impaired WH is a result of the failure of normal progression through the phases [ 26 ]. In the last few years, an increasing number of reports have evaluated the effects of pharmacologic or physical modulation of tissue repair [ 27 , 28 , 29 , 30 , 31 ].…”

Section: Discussionmentioning

confidence: 99%

Wound-Healing Promotion and Anti-Inflammatory Properties of Carvacrol Prodrugs/Hyaluronic Acid Formulations

et al. 2022

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Background. Wound healing (WH) is a complex process involving several stages, such as hemostasis, inflammation, re-epithelialization, and remodeling. Many factors can impair WH, and different pharmacological approaches were studied to date, but the increase in antibiotic resistance, invasiveness, treatment duration, and high cost, have often hampered the resolution of the wound. In this study, we investigated the possible application of water-soluble carvacrol prodrugs (WSCPs) and hyaluronic acid (HA) and their formulations (WSCPs/HA) to improve WH and regulate the inflammatory response. Materials and methods. Firstly, the cytotoxicity of 0.1, 1 and 10 µg/mL of HA, WSCPs and WSCPs/HA formulations were evaluated on HaCaT cells and THP-1 cell lines. The ability of WSCPs/HA formulations to modulate wound repair was evaluated in an in vitro model of WH, using HaCaT cells at 6, 18, and 24 h. The expression of WH mediators, after wound closure was determined by qRT-PCR. Following, we polarized THP-1 cells in M1/M2-like macrophages and tested the anti-inflammatory properties of WSCPs/HA formulations. After, we tested the in vitro WH model for the effects of conditioned medium (CM) from M1/M2-like cells cultured in the presence of WSCPs/HA. Results. Results showed that WSCPs/HA formulations were able to significantly raise the wound closure rate, compared to the single constituents, promoting a complete wound closure after 18 h for WSCP1/HA (10 µg/mL) and after 24 h for WSCP2/HA (10 µg/mL), modulating the MMPs, TGFβ, and COX-2 gene expression. The effects of CM derived from M1/M2 polarized cells cultured in the presence of WSCPs/HA determined WH regulation, with a better ability of the WSCP2/HA formulation to modulate the time-dependent expression of reparative and inflammatory mediators. Conclusion. Our data underline the possible application of WSCPs/HA formulations as bioactive agents for the regulation of the wound repair process by the modulation of inflammatory and remodeling phases, affecting the activity of immune cells.

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Challenges in Healing Wound: Role of Complementary and Alternative Medicine

Abstract: Graphical AbstractChallenges in healing wound: role of complementary and alternative medicine.

Cited by 134 publications

References 84 publications

Wound Healing Properties of Jasione montana Extracts and Their Main Secondary Metabolites

Wound Healing Properties of Jasione montana Extracts and Their Main Secondary Metabolites

Investigation Driven by Network Pharmacology on Potential Components and Mechanism of DGS, a Natural Vasoprotective Combination, for the Phytotherapy of Coronary Artery Disease

Wound-Healing Promotion and Anti-Inflammatory Properties of Carvacrol Prodrugs/Hyaluronic Acid Formulations

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