The article examines the current issue of implementing artificial intelligence (AI) technologies in modern medical practice with a special focus on the role of nursing staff. The key areas of AI application in healthcare, the existing regulatory framework in the Russian Federation, and ethical aspects of using intelligent systems in medicine are analyzed. Particular attention is paid to the transformation of nurses’ professional competencies in the context of healthcare digitalization. Based on the analysis of current practices and technology development prospects, recommendations are proposed for adapting educational programs and professional standards for nursing staff.