Chamaecyparis obtusa (Siebold &amp; Zucc.) Endl. leaf extracts prevent inflammatory responses via inhibition of the JAK/STAT axis in RAW264.7 cells

Kwon, Yong Jin; Seo, Eun Bi; Kim, Seul Ki; Noh, Kum Hee; Lee, Haeri; Joung, Yeo Won; Shin, Hyun Mu; Jang, Young Ah; Kim, Yu Mi; Lee, Jin Tae; Ye, Sang Kyu

doi:10.1016/j.jep.2021.114493

Cited by 9 publications

(8 citation statements)

References 39 publications

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“…In addition, it was confirmed that Platycodon grandifloras -fermented extracts showed an improved cell viability and reduced cytotoxicity compared to existing extracts [ 41 ]. In our previous study, we compared and evaluated the anti-inflammatory effects according to the COL extraction method in LPS-induced RAW264.7 cells and confirmed that COL EtOH extracts had excellent anti-inflammatory effects, but they show cytotoxicity at low concentrations [ 42 ]. In the present study, we confirmed that 70COLGA alleviated the high cytotoxicity of 70COL using bioconversion technology ( Figure 2 a).…”

Section: Discussionmentioning

confidence: 98%

Anti-Inflammatory Effects of Chamaecyparis obtusa (Siebold & Zucc.) Endl. Leaf Extract Fermented by Ganoderma applanatum Mycelia

Kim,

Kwon,

Jang

2024

Pharmaceutics

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Corticosteroids are commonly used anti-inflammatory agents. However, their prolonged use can lead to side effects. Therefore, the development of natural compounds with minimal side effects is necessary. This study was performed to investigate the anti-inflammatory effects and mechanisms of action of Chamaecyparis obtusa (Siebold & Zucc.) Endl. leaf (COL), bioconverted using Ganoderma applanatum (G. applanatum) in lipopolysaccharide (LPS)-induced RAW264.7 cells. The COL 70% EtOH extract fermented by G. applanatum (70COLGA) improved the high cytotoxicity of 70% EtOH extracts (70COL). When RAW264.7 cells were pre-treated with 100 and 200 μg/mL of 70COLGA for 2 h and then treated with LPS for 16 h, LPS induced the production of nitric oxide (NO), and the expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) were significantly inhibited. When RAW264.7 cells were pre-treated with 100 and 200 μg/mL of 70COLGA for 2 h and then treated with LPS for 4 h, the phosphorylation of signal transducers and activators of transcription (STAT) was markedly decreased. In addition, 70COLGA markedly suppressed the production of the inflammatory cytokines interleukin (IL)-1β and IL-6 in LPS-induced RAW264.7 cells. Analysis of pro-inflammatory molecules using cytokine arrays showed that macrophage inflammatory protein (MIP)-2, granulocyte–macrophage colony-stimulating factor (GM-CSF), granulocyte colony-stimulating factor (G-CSF) and IL-27 expressions were also suppressed by 200 μg/mL of 70COLGA in LPS-induced RAW264.7 cells. These results demonstrate that 70COLGA significantly prevented inflammatory responses by inhibiting the secretion of pro-inflammatory molecules in LPS-induced RAW264.7 cells. When RAW264.7 cells were pre-treated with 100 and 200 μg/mL of 70COLGA for 2 h and then treated with LPS-conditioned medium (LPS-CM) for 30 min, 70COLGA directly inhibited STAT activation. In summary, our findings suggest that 70COLGA has therapeutic potential for the treatment of inflammatory diseases.

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Section: Discussionmentioning

confidence: 98%

Anti-Inflammatory Effects of Chamaecyparis obtusa (Siebold & Zucc.) Endl. Leaf Extract Fermented by Ganoderma applanatum Mycelia

Kim,

Kwon,

Jang

2024

Pharmaceutics

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“…NO concentration was determined by the Griess assay according to a previous report with minor modification . The RAW264.7 cells (3 × 10 4 cells/well) were plated into 96-well plates.…”

Section: Methodsmentioning

confidence: 99%

“…NO concentration was determined by the Griess assay according to a previous report with minor modification. 23 The RAW264.7 cells (3 × 10 4 cells/well) were plated into 96-well plates. After 12 h of incubation, the cells were pretreated with the sample drugs individually for 2 h and then co-treated with LPS (1 μg/ mL) for 24 h. Subsequently, the cells were discarded, and the aspirated medium was mixed with Griess reagent 1 and Griess reagent 2 in a 1:1:1 ratio.…”

Section: ■ Conclusionmentioning

confidence: 99%

Catalyst-Free Cascade Annulation of Enaminones and Aryl Diazonium Tetrafluoroboronates for Cinnoline Synthesis and the Anti-Inflammatory Activity Study

et al. 2023

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A simple and concise method for the synthesis of cinnolines has been developed by the reactions of readily available enaminones and aryl diazonium tetrafluoroboronates. The reactions run efficiently to provide cinnolines with broad diversity in the substructure by heating in dimethyl sulfoxide without using any catalyst or additive. In addition, the primary investigation of the anti-inflammatory activity of these products leads to the observation of p-chlorobenzoyl (3f) and p-nitrobenzoyl (3j) cinnolines as attractive anti-inflammatory compounds in vitro.

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“…NO concentration was determined by the Griess assay according to a previous report with some modification. 23 RAW264.7 cells (5 × 10 4 cells per well) were plated into 96-well plates. After an incubation period of 24 h, the cells were pre-treated with different drugs individually for 2 h and were cotreated with LPS (1 μg mL −1 ) for 12 h. Subsequently, the culture medium was mixed with the Griess reagent in a 1 : 1 ratio.…”

Section: Methodsmentioning

confidence: 99%

Metal-free three-component assemblies of anilines, α-keto acids and alkyl lactates for quinoline synthesis and their anti-inflammatory activity

et al. 2022

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Chamaecyparis obtusa (Siebold & Zucc.) Endl. leaf extracts prevent inflammatory responses via inhibition of the JAK/STAT axis in RAW264.7 cells

Cited by 9 publications

References 39 publications

Anti-Inflammatory Effects of Chamaecyparis obtusa (Siebold & Zucc.) Endl. Leaf Extract Fermented by Ganoderma applanatum Mycelia

Anti-Inflammatory Effects of Chamaecyparis obtusa (Siebold & Zucc.) Endl. Leaf Extract Fermented by Ganoderma applanatum Mycelia

Catalyst-Free Cascade Annulation of Enaminones and Aryl Diazonium Tetrafluoroboronates for Cinnoline Synthesis and the Anti-Inflammatory Activity Study

Metal-free three-component assemblies of anilines, α-keto acids and alkyl lactates for quinoline synthesis and their anti-inflammatory activity

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