Changed Expression of Cytoskeleton Proteins During Lung Injury in a Mouse Model of Streptococcus pneumoniae Infection

Abstract

Infections by Streptococcus pneumoniae are a major cause of morbidity and mortality worldwide, often causing community-acquired pneumonia, otitis media and also bacteremia and meningitis. Studies on S. pneumoniae are mainly focused on its virulence or capacity to evade the host immune system, but little is known about the injury caused in lungs during a pneumococcal infection. Herein we investigated this issue comparing the proteome profile of lungs from S. pneumoniae-infected mice with control mice by means of difference gel electrophoresis (DIGE) technology. In order to obtain reliable results three biological replicas were used, and four technical replicas were carried out in each biological replica. Proteomic comparison was performed at two time points: 24 and 48 h post infection. A total of 91 proteins were identified with different abundance. We found important changes in the protein profiles during pneumococcal infection mainly associated with regulation of vesicle-mediated transport, wound healing, and cytoskeleton organization. In conclusion, the results obtained show that the cytoskeleton of the host cell is modified in S. pneumoniae infection.

Proteomics Landscape of Host-Pathogen Interaction in Acinetobacter baumannii Infected Mouse Lung

“…Ferrer-Navarro et al compared the proteome profiles of mouse lungs infected by Streptococcus pneumoniae (Gram-positive bacteria) with control mice at 24 and 48 h postinfection by 2D-differential gel electrophoresis (2D-DIGE). 91 differentially expressed proteins were identified, and the analysis showed that the cytoskeleton of host lung tissue cells is modified in S. pneumoniae infection (Ferrer-Navarro et al, 2018). Seddigh et al (2017) applied a label-free proteome analysis of alveolar epithelial cells infected with A. fumigatus (fungus), which study revealed the key role of IL-41 in the host defense.…”