Changes in Acylglycerols Composition, Quality Characteristics and In vivo Effects of Dietary Pumpkin Seed Oil upon Thermal Oxidation

Abstract

This study was aimed to determine the acylglycerols composition, quality characteristics, and protective role of dietary pumpkin seed oil (PSO) in rabbits. PSO was thermally oxidized and analyzed for quality characteristics and acylglycerols composition using reversed phase high performance liquid chromatography with diode array detection (HPLC-DAD). Oxidized and un-oxidized oil samples were fed to the rabbits in different doses for 2 weeks. The changes in the serum biochemistry, hematology, and liver histology were studied. The levels of quality parameters such peroxide value (PV), anisidine value (AV), total phenolic contents (TPC), thiobarbituric acid reactive substances (TBARS), conjugated dienes (CD) and conjugated trienes (CT) significantly increased with thermal treatment. HPLC analyses revealed 10 individual triacylglycerols (TAGs), total di-acylglycerols (DAGs), mono-acylglycerols (MAGs), and total oxidized TAGs. Trilinolein (LLL), 1-oleoyl-2,3-dilinolinoyl glycerol (OLL), triolein (OOO) and 1,2-distearoyl-3-palmitoyl glycerol (SSP) were present in higher amounts and decreased with thermal treatment. Animal's studies showed that oxidized oils decreased the whole body weight, which was ameliorated by the co-administration of un-oxidized oils. The levels of serum biochemical parameters were improved by co-administration of pumpkin seed oils. There were no significant effects of both oxidized and un-oxidized PSO on the hematological and histological parameters of rabbits. In conclusion, nutritionally important triacylglycerols were present in PSO with protective role against the toxicity of its corresponding oxidized oils.

Supplementation with extract of pumpkin seeds exerts estrogenic effects upon the uterine, serum lipids, mammary glands, and bone density in ovariectomized rats

“…Estrogen plays a pivotal role not only in the reproductive system but also in certain vital health condition (Makni et al, ). Estrogen deficiency will undoubtedly cause several physiological changes and pathological conditions, such as osteoporosis, cardiovascular diseases, dyslipidemia, sleep disorders, and joint pain (Gossell‐Williams, Lyttle, Clarke, Gardner, & Simon, ; Zeb & Ahmad, ). Therefore, several efforts should be made to maintain estrogen levels within the female body.…”

Perfil cromatográfico diferencial de triglicéridos en semillas de calabazas (Cucurbita spp.) silvestres y domesticadas

“…The bands intervals the in the tracks are placed in agreement to the increase of the band values with Rf between the sample application site (value 0.0) and the solvent front at the end of the development (value 1.0). Subsequently, in the calibration mode of bands in linear function, the main reference TG (18:2/18:2/18:2) was observed in most pumpkin seed extracts (Zeb & Ahmad, 2017). The baseline of all leads was defined to display them in the form of a chromatogram.…”

“…Wild pumpkin species TG profile has been established to date (Ali et al, 2017;Azimova, Glushenkova, & Vinogradova, 2011;Neđeral et al, 2012;Zeb & Ahmad, 2017), although there is no comparison of TG composition in domesticated versus wild pumpkin seeds. Some of the methods for the study of TG profile in pumpkins comprise: the use of silica gel plates impregnated with silver nitrate and subsequent evaluation of the fatty acid methyl esters by gas chromatography/mass spectrometry (GC/MS) (Yoshida et al, 2004); the carbon equivalent number (ECN42), International Olive Council (IOC) chromatographic method (Bardaa et al, 2016); the high performance liquid chromatography (HPLC) in its modalities (Ali et al, 2017;Zeb & Ahmad, 2017). and laser-assisted matrix desorption-ionization time-of-flight (MALDI-TOF-MS) (Beneito et al, 2020).…”

“…A la fecha se ha establecido el perfil de los TG de especies de calabazas silvestres (Ali et al, 2017;Azimova, Glushenkova, & Vinogradova, 2011;Neđeral et al, 2012;Zeb & Ahmad, 2017), aunque no existe una comparación de la composición de TG en semillas de calabazas domesticadas vs. silvestres. Algunos de los métodos para el estudio del perfil de los TG en calabazas comprenden el uso de placas de gel de sílice impregnadas con nitrato de plata y la evaluación posterior de ésteres de metilo de ácidos grasos resultado de su hidrólisis por cromatografía de gases/espectrometría de masas (GC/MS) (Yoshida et al, 2004); el número de equivalente de carbono (ECN42), el método cromatográfico del Consejo Oleícola Internacional (IOC) (Bardaa et al, 2016); la cromatografía líquida de alta eficiencia (HPLC) en sus modalidades (Ali et al, 2017;Zeb & Ahmad, 2017); así como matrix asistida por láser de desorción -ionización tiempo de vuelo (MALDI-TOF-MS) (Beneito et al, 2020). Hay varios mecanismos para aislamiento y detección de los TG, pero son muy laboriosos; por tanto, no son adecuados para labores de rutina (Cossignani, Pollini, & Blasi, 2019).…”

A Review: The Emerging Nutraceutical Potential of Pumpkin Seeds

“…Further study revealed that pumpkin seeds exhibited estrogenic-like effects such as regulating lipid metabolism, bone remodeling, mammary gland and uterus epithelial cells development. Phytoestrogen components were the key role in inhibiting cardiovascular outcomes and balancing the plasma lipids level such as total cholesterol, low-density lipoprotein (LDL), highdensity lipoprotein (HDL), and triglyceride (Gossell-Williams, et al, 2008;Jones, et al, 2000;Zeb and Ahmad, 2017). In conclusion, phytoestrogens and tocopherols presented in pumpkin seeds contribute to their estrogenic-like effects.…”