Changes in antioxidant enzyme activities in detached leaves of cucumber exposed to chilling

Lee, Min Ah; Chun, Hyun Sik; Kim, Jin Woo; Lee, Hyub; Lee, Dong Hee; Lee, Chin Bum

doi:10.1007/bf03030641

Cited by 12 publications

(8 citation statements)

References 28 publications

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“…Xia et al showed that ROS is a signaling molecule involved in the response of cucumbers to low-temperature and paraquat stress [ 32 ]. MDA is a product of lipid peroxidation and the extent of membrane damage can be reflected by MDA levels in stressed tissues [ 33 ]. POD can maintain balance by removing excess ROS, which helps plants to endure stress conditions [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis Reveals Key Cold-Stress-Responsive Genes in Winter Rapeseed (Brassica rapa L.)

Coulter

Liu³

et al. 2019

IJMS

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Low ambient air temperature limits the growth and selection of crops in cold regions, and cold tolerance is a survival strategy for overwintering plants in cold winters. Studies of differences in transcriptional levels of winter rapeseed (Brassica rapa L.) under cold stress can improve our understanding of transcript-mediated cold stress responses. In this study, two winter rapeseed varieties, Longyou-7 (cold-tolerant) and Lenox (cold-sensitive), were used to reveal morphological, physiological, and transcriptome levels after 24 h of cold stress, and 24 h at room temperature, to identify the mechanism of tolerance to cold stress. Compared to Lenox, Longyou-7 has a shorter growth period and greater belowground mass, and exhibits stronger physiological activity after cold stress. Subsequently, more complete genomic annotation was obtained by sequencing. A total of 10,251 and 10,972 differentially expressed genes (DEG) were identified in Longyou-7 and Lenox, respectively. Six terms closely related to cold stress were found by the Gene Ontology (GO) function annotation. Some of these terms had greater upregulated expression in Longyou-7, and the expression of these genes was verified by qRT-PCR. Most of these DEGs are involved in phenylpropanoid biosynthesis, plant hormone signal transduction, ribosome biogenesis, MAPK signaling pathway, basal transcription factors, and photosynthesis. Analysis of the genes involved in the peroxisome pathway revealed that Longyou-7 and Lenox may have different metabolic patterns. Some transcription factors may play an important role in winter rapeseed tolerance to cold stress, and Longyou-7 is slightly slower than Lenox. Our results provide a transcriptome database and candidate genes for further study of winter rapeseed cold stress.

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Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis Reveals Key Cold-Stress-Responsive Genes in Winter Rapeseed (Brassica rapa L.)

Coulter

Liu³

et al. 2019

IJMS

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“…Caso isso fosse evidente, tais figuras apresentariam gradientes de concentração ou atividade dos antioxidantes ao longo do período de exposição. O perfil dos antioxidantes não aponta, igualmente, indícios de aclimatação das plantas às condições de exposição da casa de vegetação, o que seria verificado caso os níveis dos mesmos variassem pouco ao longo das amostragens (Barnes et al 1999 (Chaitanya et al 2002, Lee et al 2004, Takac 2004.…”

Section: Discussionunclassified

“…Certamente, alguns fatores de variação devem ser previamente conhecidos para que se tenha certeza da ativação do sistema de respostas antioxidativas em plantas que crescem em ambientes urbanos, pois se sabe que tais defesas variam não somente entre espécies, como também entre plantas da mesma espécie em diferentes estádios de desenvolvimento e entre folhas da mesma planta, em fases distintas de desenvolvimento (Klumpp et al 2000). Além disso, as espécies também respondem às variações ambientais ao longo das estações do ano (Chaitanya et al 2002, Kuk et al 2003, Lee et al 2004, Rivero et al 2004, Zhou & Zhao 2004.…”

Section: Introductionunclassified

Variação sazonal em antioxidantes em folhas de plantas jovens de Caesalpinia echinata Lam. (pau-brasil)

et al. 2005

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-(Seasonal variation in antioxidants in leaves of young plants of Caesalpinia echinata Lam. (brazilwood)). The urban environmental factors generate conditions of oxidative stress in plants. Cellular antioxidants, such as ascorbic acid (AA), thiols (Ti), peroxidases (POD) and superoxide dismutase (SOD) are induced to different levels depending on their capacity to tolerate stressing conditions. In the first part of this study we determined the profile of these defenses in leaves of different ages of young plants of C. echinata. The levels of antioxidants were similar in all leaves analyzed, independently of the age. Secondly, we evaluated the same antioxidants and glutathione (GSH) in 130 young plants kept in a greenhouse with constant monitoring of temperature, relative humidity and irradiance during 18 months, in order to establish the seasonal variations occurring in response to climatic factors. During this period, leaf antioxidants were analyzed every three months. In general, AA concentrations and POD activity in C. echinata were higher than the levels reported for other tropical tree species. Seasonal variations were observed. GSH concentration and POD activity were significantly influenced by temperature and SOD by temperature and humidity.Key words -ascorbic acid, Caesalpinia echinata, glutathione, peroxidases, superoxide dismutase, thiols RESUMO -(Variação sazonal em antioxidantes em folhas de plantas jovens de Caesalpinia echinata Lam. (pau-brasil)). O ambiente urbano pode gerar condições de estresse oxidativo nas plantas, levando à estimulação de antioxidantes como ácido ascórbico (AA), tióis (Ti), peroxidases (POD) e superóxido dismutase (SOD), em diferentes níveis, de acordo com sua capacidade de tolerar tais condições. Este estudo, em sua primeira fase, buscou conhecer o perfil destas defesas em folhas de plantas jovens de C. echinata com diferentes graus de desenvolvimento. Os níveis dos antioxidantes foram similares em todas as folhas analisadas. Em uma segunda fase, tais antioxidantes e a glutationa (GSH) foram avaliados em 130 plantas mantidas em casa de vegetação com monitoramento constante de temperatura, umidade relativa e irradiância, por um período de 18 meses, para estabelecer se variações sazonais nessas defesas ocorrem em resposta a fatores climáticos. Nesse período, os antioxidantes foliares foram analisados a cada três meses. Em geral, a concentração de AA e a atividade da POD foram altas em C. echinata, quando comparadas com as de outras espécies arbóreas tropicais. Houve variações nas defesas ao longo do tempo. A concentração de GSH e a atividade da POD mostraram ser influenciadas por mudanças na temperatura e a SOD foi estimulada em resposta à temperatura e à umidade relativa.Palavras-chave -ácido ascórbico, Caesalpinia echinata, glutationa, peroxidases, superóxido dismutase, tióis IntroduçãoA exploração descontrolada do pau-brasil (Caesalpinia echinata Lam., LeguminosaeCaesalpiniodeae), nos primeiros séculos da colonização, contribuiu significativamente para a redução de ...

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“…AOS include superoxide anion (02), the hydroxyl radical ( 9 OH), hydrogen peroxide (H202), and singlet oxygen (102). *Corresponding author; fax +82-55-758-5110 e-mail jjl@gshp.gsnu.ac.kr The increase in AOS, which are also produced during normal aerobic metabolism, seems to occur in response to all abiotic stresses, including chilling (Lee et al, 2004), heavy metal toxicity (Ali et al, 2002), drought (Smirnoff, 1993), and salt Gossett et al, 1996). Failure to quench or inactivate the AOS may lead to the degradation of macromolecules in cells as membrane lipids, proteins, and DNA (EIstner, 1982;Smirnoff, 1993).…”

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confidence: 98%

Effects of NaCl stress on germination, antioxidant responses, and proline content in two rice cultivars

Sohn¹,

Lee

Kang

et al. 2005

J. Plant Biol.

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We investigated the physiological and biochemical bases for salt tolerance in two rice (Oryza sativa L.) cultivars --rel. atively salt-tolerant ~Dongjin ~ and salt-sensitive 'Kumnam'. Salinized hydroponic cultures were studied at the germination and seedling stages. NaCI inhibited germination more severely in 'Kumnam' than in 'Dongjin'. Increasing the salt concentration also deterred growth to a larger extent in the former. Moreover, the leaves of ~Kumnam' exhibited greater increases in lipid peroxidation and Na § accumulation than those of 'Dongjin' under stress. The activities of constitutive and salt-induced superoxide dismutase (SOD, EC 1.15.1.1) and ascorbate peroxidase (AP, EC 1.11.1.11 ) were also higher in ~Kumnam', while only catalase (CAT, EC 1.11.1.6) activity was slightly higher in stressed plants of ~Dongjin'. The positive correlation between leaf proline levels and NaCI concentration was more evident in ~Kumnam'. However, 'Dongjin' seeds, which had higher germinability in the presence of NaCI, also contained more proline. These results suggest that the higher salt tolerance in 'Dongjin' seedlings could be ascribed to their lower NaCI accumulations in the leaves. This presumably is due to reductions in the uptake or transport rates of saline ions to the shoots from the roots. Finally, we believe that the higher germination rate by 'Dongjin' is caused by its higher seed proline content.

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Changes in antioxidant enzyme activities in detached leaves of cucumber exposed to chilling

Cited by 12 publications

References 28 publications

Transcriptome Analysis Reveals Key Cold-Stress-Responsive Genes in Winter Rapeseed (Brassica rapa L.)

Transcriptome Analysis Reveals Key Cold-Stress-Responsive Genes in Winter Rapeseed (Brassica rapa L.)

Variação sazonal em antioxidantes em folhas de plantas jovens de Caesalpinia echinata Lam. (pau-brasil)

Effects of NaCl stress on germination, antioxidant responses, and proline content in two rice cultivars

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