Changes in aquaporins mRNA expression and liquid storage at 17°C: A potential biomarker of boar sperm quality?

Quintana, Marina Castany; Gardela, Jaume; Ruiz-Conca, Mateo; López‐Béjar, Manel; Martínez, Cristina A.; Rodriguez‐Martinez, Heriberto; Álvarez‐Rodríguez, Manuel

doi:10.1111/rda.14134

Reprod Domestic Animals

2022

DOI: 10.1111/rda.14134

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Changes in aquaporins mRNA expression and liquid storage at 17°C: A potential biomarker of boar sperm quality?

Marina Castany Quintana

Jaume Gardela

Mateo Ruiz-Conca

et al.

Abstract: In the swine industry, most artificial inseminations (AI) are carried out using extended liquid semen as sperm cryopreservation remains suboptimal, and with individual differences are becoming more marked as storage time increases (De Ambrogi et al., 2006).However, AI doses stored for more than 24 hr have been related to decreased fertility (Christensen et al., 2004). Unlike spermatozoa in seminal plasma, which retain motility for a few hours, AI doses pursue to control not only the reduction of the metabolic … Show more

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Cited by 3 publications

References 17 publications

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Aqp4a and Trpv4 mediate regulatory cell volume increase for swimming maintenance of marine fish spermatozoa

Castro-Arnau,

Chauvigné,

Toft-Bertelsen

et al. 2024

Cell. Mol. Life Sci.

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Volume regulation is essential for cell homeostasis and physiological function. Amongst the sensory molecules that have been associated with volume regulation is the transient receptor potential vanilloid 4 (TRPV4), which is a non-selective cation channel that in conjunction with aquaporins, typically controls regulatory volume decrease (RVD). Here we show that the interaction between orthologous AQP4 (Aqp4a) and TRPV4 (Trpv4) is important for regulatory volume increase (RVI) in post-activated marine fish spermatozoa under high osmotic stress. Based upon electrophysiological, volumetric, and in vivo and ex vivo functional experiments using the pharmacological and immunological inhibition of Aqp4a and Trpv4 our model suggests that upon ejaculation and exposure to the hypertonic seawater, spermatozoon shrinkage is initially mediated by water efflux through Aqp1aa in the flagellar tail. The shrinkage results in an increase in intracellular Ca2+ concentration, and the activation of sperm motility and a Na+/K+/2Cl− (NKCC1) cotransporter. The activity of NKCC1 is required for the initiation of cell swelling, which secondarily activates the Aqp4a-Trpv4 complex to facilitate the influx of water via Aqp4a-M43 and Ca2+ via Trpv4 and L-type channels for the mediation of RVI. The inhibitory experiments show that blocking of each of these events prevents either shrinkage or RVI. Our data thus reveal that post-activated marine fish spermatozoa are capable of initiating RVI under a high hypertonic stress, which is essential for the maintenance of sperm motility.

show abstract

Aqp4a and Trpv4 mediate regulatory cell volume increase for swimming maintenance of marine fish spermatozoa

Castro-Arnau,

Chauvigné,

Toft-Bertelsen

et al. 2024

Cell. Mol. Life Sci.

View full text Add to dashboard Cite

show abstract

Genomic, transcriptomic and epigenomic analysis towards the understanding of porcine semen quality traits. Past, current and future trends

Sa,

Gòdia,

Lewis

et al. 2024

Animal Reproduction Science

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Changes in aquaporins mRNA expression and liquid storage at 17°C: A potential biomarker of boar sperm quality?

Quintana

Gardela

Ruiz-Conca

et al. 2022

Reprod Domestic Animals

View full text Add to dashboard Cite

In the swine industry, most artificial inseminations (AI) are carried out using extended liquid semen as sperm cryopreservation remains suboptimal, and with individual differences are becoming more marked as storage time increases (De Ambrogi et al., 2006).However, AI doses stored for more than 24 hr have been related to decreased fertility (Christensen et al., 2004). Unlike spermatozoa in seminal plasma, which retain motility for a few hours, AI doses pursue to control not only the reduction of the metabolic activity by

show abstract

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Changes in aquaporins mRNA expression and liquid storage at 17°C: A potential biomarker of boar sperm quality?

Cited by 3 publications

References 17 publications

Aqp4a and Trpv4 mediate regulatory cell volume increase for swimming maintenance of marine fish spermatozoa

Aqp4a and Trpv4 mediate regulatory cell volume increase for swimming maintenance of marine fish spermatozoa

Genomic, transcriptomic and epigenomic analysis towards the understanding of porcine semen quality traits. Past, current and future trends

Changes in aquaporins mRNA expression and liquid storage at 17°C: A potential biomarker of boar sperm quality?

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