Changes in Calcium-Dependent Protein Kinase Activity during in Vitro Tuberization in Potato

MacIntosh, Gustavo C.; Ulloa, Rita M.; Raı́ces, Marcela; Téllez-Iñón, Marı́a T.

doi:10.1104/pp.112.4.1541

Cited by 47 publications

(44 citation statements)

References 34 publications

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“…This hypothesis is in agreement with the stimulus effect of Ca 2+ on tuberisation as previously reported (Balamani et al, 1986;Macintosh et al, 1996).…”

Section: Discussionsupporting

confidence: 91%

Inductive and noninductive conditions on in vitro tuberisation and microtuber dormancy in potato (Solanum tuberosum subspeciestuberosum and subspeciesandigena)

Vecchio¹,

Benedettelli²,

Andrenelli³

et al. 2000

Potato Res

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SummaryThe effect of growth conditions (medium and photoperiod) on in vitro tuberisation and microtuber dormancy of the cv. D6sirfe (Solanum tuberosum subspecies tuberosum) and Imilla Negra (Solanum tuberosum subspecies andigena) was evaluated. The short photoperiod and presence of CCC in the medium reduced the dry matter of plants as well as the size of microtubers. The two cultivars showed a difference, in favour of D6sir6e, in the percentage of plants having more than one tuber and in the average time of sprouting after storage. The average time of sprouting was more rapidly obtained under long days and in a medium without CCC. The CCC and short days caused the same dormancy conditions and the same K + quantity in the microtubers. The microtubers mineral composition is involved in the dormancy status.

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“…This hypothesis is in agreement with the stimulus effect of Ca 2+ on tuberisation as previously reported (Balamani et al, 1986;Macintosh et al, 1996).…”

Section: Discussionsupporting

confidence: 91%

Inductive and noninductive conditions on in vitro tuberisation and microtuber dormancy in potato (Solanum tuberosum subspeciestuberosum and subspeciesandigena)

Vecchio¹,

Benedettelli²,

Andrenelli³

et al. 2000

Potato Res

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“…CDPK activity was determined in soluble, microsomal and enriched plasma membrane fractions as described (MacIntosh et al 1996). The reaction mixture contained 20 mM Tris-HCl pH 7.5, 10 mM MgCl 2 , 50 lM [c-32 P] ATP (specific activity 100 cpm/pmol), 10 mM 2-mercaptoethanol, 25 lM Syntide-2, with the addition of 1 mM EGTA or 5 lM CaCl 2 .…”

Section: Methodsmentioning

confidence: 99%

“…In previous works we reported a temporal correlation between an increase in CDPK activity and the morphological changes associated with the onset of tuber development using in vitro cultured potato stolons (MacIntosh et al 1996). We analyzed the expression and activity of potato CDPK isoforms that follow different expression profiles during tuberization (Raices et al 2003a).…”

Section: Introductionmentioning

confidence: 98%

Genomic and functional characterization of StCDPK1

et al. 2009

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StCDPK1 is a calcium dependent protein kinase expressed in tuberizing potato stolons and in sprouting tubers. StCDPK1 genomic sequence contains eight exons and seven introns, the gene structure is similar to Arabidopsis, rice and wheat CDPKs belonging to subgroup IIa. There is one copy of the gene per genome and it is located in the distal portion of chromosome 12. Western blot and immunolocalization assays (using confocal and transmission electron microscopy) performed with a specific antibody against StCDPK1 indicate that this kinase is mainly located in the plasma membrane of swelling stolons and sprouting tubers. Sucrose (4-8%) increased StCDPK1 protein content in non-induced stolons, however the amount detected in swelling stolons was higher. Transgenic lines with reduced expression of StCDPK1 (beta 7) did not differ from controls when cultured under multiplication conditions, but when grown under tuber inducing conditions some significant differences were observed: the beta 7 line tuberized earlier than controls without the addition of CCC (GA inhibitor), developed more tubers than wild type plants in the presence of hormones that promote tuberization in potato (ABA and BAP) and was more insensitive to GA action (stolons were significantly shorter than those of control plants). StCDPK1 expression was induced by GA, ABA and BAP. Our results suggest that StCDPK1 plays a role in GA-signalling and that this kinase could be a converging point for the inhibitory and promoting signals that influence the onset of potato tuberization.

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“…CDPKs have a C-terminal CaM-like elongation factor-hand motif enabling Ca 2ϩ binding that leads to conformational changes and activation of the N-terminal kinase domain. They have been implicated to play a role during tuberization of potato (MacIntosh et al, 1996) and during the gibberellic acid response in barley aleurone (Ritchie and Gilroy, 1998). Expression of some CDPKs is induced by physical stress, by salt stress, by CaCl 2 in mung bean (Botella et al, 1996), by osmotic stress in sorghum (Pestenacz and Erdei, 1996), and by phytohormones, methyl jasmonate, wounding, fungal elicitors, chitosan, and NaCl in tobacco leaves (Yoon et al, 1999).…”

mentioning

confidence: 99%

Spatio-Temporal Accumulation and Activity of Calcium-Dependent Protein Kinases during Embryogenesis, Seed Development, and Germination in Sandalwood

2000

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Western-blot analysis and protein kinase assays identified two Ca 2؉ -dependent protein kinases (CDPKs) of 55 to 60 kD in soluble protein extracts of embryogenic cultures of sandalwood (Santalum album L.). However, these sandalwood CDPKs (swCDPKs) were absent in plantlets regenerated from somatic embryos. swCDPKs exhibited differential expression (monitored at the level of the protein) and activity in different developmental stages. Zygotic embryos, seedlings, and endosperm showed high accumulation of swCDPK, but the enzyme was not detected in the soluble proteins of shoots and flowers. swCDPK exhibited a temporal pattern of expression in endosperm, showing high accumulation and activity in mature fruit and germinating stages; the enzyme was localized strongly in the storage bodies of the endosperm cells. The study also reports for the first time to our knowledge a post-translational inhibition/inactivation of swCDPK in zygotic embryos during seed dormancy and early stages of germination. The temporal expression of swCDPK during somatic/zygotic embryogenesis, seed maturation, and germination suggests involvement of the enzyme in these developmental processes.

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Changes in Calcium-Dependent Protein Kinase Activity during in Vitro Tuberization in Potato

Abstract: A soluble Ca2+-dependent protein kinase (CDPK) was purified to homogeneity in potato (Solanum tuberosum L.) plants. Potato CDPK was strictly dependent on Ca2+ (one-half maximal activation 0.6 p~) and phosphorylated a wide diversity of substrates, in which

Cited by 47 publications

References 34 publications

Inductive and noninductive conditions on in vitro tuberisation and microtuber dormancy in potato (Solanum tuberosum subspeciestuberosum and subspeciesandigena)

Inductive and noninductive conditions on in vitro tuberisation and microtuber dormancy in potato (Solanum tuberosum subspeciestuberosum and subspeciesandigena)

Genomic and functional characterization of StCDPK1

Spatio-Temporal Accumulation and Activity of Calcium-Dependent Protein Kinases during Embryogenesis, Seed Development, and Germination in Sandalwood

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