Changes in cardiac proteome and metabolome following exposure to the PAHs retene and fluoranthene and their mixture in developing rainbow trout alevins

Eriksson, Andreas N.M.; Rigaud, Cyril; Rokka, Anne; Skaugen, Morten; Lihavainen, Jenna; Vehniäinen, Eeva-Riikka

doi:10.1016/j.scitotenv.2022.154846

Cited by 13 publications

(9 citation statements)

References 76 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar temporal patterns of accumulation were observed among alevins exposed to the mixture of retene and fluoranthene, as reported in our previous studies (Eriksson et al 2022a, b). Hence, the reduction in the body burden of fluoranthene, when coexposed with retene, reflects a broader and more potent activation of phase I and II metabolic processes, either transcriptomic (Eriksson et al 2022a) or proteomic (Eriksson et al 2022b), that can offset the inhibitory effect of fluoranthene upon Cyp1a.…”

Section: Resultsmentioning

confidence: 99%

“…UV-irradiation can be rejected as causative agent due to the architecture of the exposure facility (no windows), as the room was illuminated by yellow florescent light. That leaves enzymatic processes and oxidative stress as the most plausible causes; the latter being more likely due the known and established relationship between PAH toxicity and subsequently increased oxidative stress (Timme-Laragy et al 2007 ; Song et al 2019 ), altered iron metabolism (Rigaud et al 2020b ; Eriksson et al 2022b ) and activation of heat shock proteins (Räsänen et al 2012 ) in PAH exposed fish larvae. The body burden of FICZ may also increase when subsequent metabolism by Cyp1a is inhibited (Wincent et al 2012 , 2016 ).…”

Section: Resultsmentioning

confidence: 99%

“…From an aquatic toxicological perspective, the exact mechanisms of toxicity are not fully understood, even after decades of research employing multiple species of fish and types of PAHs (Heintz et al 2000 ; Brinkworth et al 2003 ; Wassenberg and Di Giulio 2004 ; Van Tiem and Di Giulio 2011 ; Clark et al 2013 ; Rigaud et al 2020a ). What is known is that different PAHs induce toxicity through different mechanisms and cause exposure and PAH specific toxicity profiles (Billiard et al 2008 ; Incardona et al 2011 ; Geier et al 2018 ; Rigaud et al 2020a , b ; Eriksson et al 2022a , b ). Additionally, heart structure and function are especially sensitive to the influence of PAH(s) during early life development of fish (Incardona et al 2011 , 2009 ; Vehniäinen et al 2016 ).…”

Section: Introductionmentioning

confidence: 99%

“…The specificity of cardiotoxicity is hypothesized to be linked to interaction(s) between PAHs, as a part of crude oil, and lipoproteins bound to the cellular membrane (Incardona 2017 ). Furthermore, mixture composition has been observed and reported to modulate PAH toxicity outcome, relative to the components (Geier et al 2018 ; Eriksson et al 2022a , b ). The best-known molecular mechanism related to PAH toxicity is that involving activation of the aryl-hydrocarbon receptor 2 (AhR2) (Massarsky et al 2016 ; Doering et al 2019 ).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Endogenous AhR agonist FICZ accumulates in rainbow trout (Oncorhynchus mykiss) alevins exposed to a mixture of two PAHs, retene and fluoranthene

et al. 2022

Self Cite

View full text Add to dashboard Cite

Multiple studies have reported synergized toxicity of PAH mixtures in developing fish larvae relative to the additive effect of the components. From a toxicological perspective, multiple mechanisms are known to contribute to synergism, such as altered toxicodynamics and kinetics, as well as increased oxidative stress. An understudied contributor to synergism is the accumulation of endogenous metabolites, for example: the aryl hydrocarbon receptor 2 (AhR2) agonist and tryptophan metabolite 6-Formylindolo(3,2-b)carbazole (FICZ). Fish larvae exposed to FICZ, alongside knock-down of cytochrome p450 (cyp1a), has been reported to induced symptoms of toxicity similar to those observed following exposure to PAHs or the dioxin 2,3,7,8-tetrachlorodibenzo-p-dioxin. Here, we explored if FICZ accumulates in newly hatched rainbow trout alevins (Oncorhynchus mykiss) exposed to two PAHs with different properties: retene (potent AhR2 agonist) and fluoranthene (weak AhR2 agonist and Cyp1a inhibitor), either alone or as a binary mixture for 3 and 7 days. We found that exposure to the mixture resulted in accumulation of endogenous FICZ, synergized the blue sac disease index (BSD), and altered the body burden profiles of the PAHs, when compared to the alevins exposed to the individual components. It is thus very plausible that accumulation of endogenously derived FICZ contributed to the synergized BSD index and toxicity in exposed alevins. Accumulation of endogenously derived FICZ is a novel finding that extends our general understanding on PAHs toxicity in developing fish larvae, while at the same time highlighting why environmental risk assessment of PAHs should not be based solely results from the assessment of individual compounds.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Endogenous AhR agonist FICZ accumulates in rainbow trout (Oncorhynchus mykiss) alevins exposed to a mixture of two PAHs, retene and fluoranthene

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…Population studies have found that PAH affects multiple systems such as the nervous (Feng et al 2022), cardiovascular (Mallah et al 2022), and renal (Rahman et al 2022) systems, and may lead to the occurrence of chronic diseases such as diabetes (Srivastava 2022) and hypertension (Wang et al 2022a). Moreover, the mixture toxicity of PAH with a series of compounds, is also well studied (Eriksson et al 2022, Kreutzer et al 2022, Wallace et al 2022. Few of these studies have focused on the mixed toxicity of PAH by adding HAA for analysis.…”

Section: Discussionmentioning

confidence: 99%

Association Between Urinary Heterocyclic Aromatic Amines and Mortality in Adults

Zhang

Lin

et al. 2022

Preprint

View full text Add to dashboard Cite

Heterocyclic aromatic amines (HAA) are mutagenic compounds; however, their long-term effects on humans remain poorly studied. This study investigated the association between HAA concentration and mortality in adults. Data from the National Health and Nutrition Examination Survey 2013-2014 were linked to mortality data from 2019 and analyzed using Cox regression analysis to determine the association of different HAA with all-cause, cardiovascular disease (CVD), and cancer mortality. Age, sex, education level, race, poverty income ratio, body mass index, smoking status, drinking status, and serum cotinine were included as covariates in three different Cox regression models. This study included 1,713 adults with a median age of 47 years (49.5% men). During a median follow-up at 71 months, 100 deaths occurred. Owing to detection limits, only four had discernible detection rates and were included in our analysis. Among them, only norharman concentration was significantly associated with all-cause and CVD mortality. The hazard ratio (HR) was 1.50 (95% confidence interval: 1.10–2.04) for all-cause mortality when analyzing norharman concentration as a natural log-transformed continuous variable and 3.33 (95% confidence interval: 2.24–4.95) for CVD mortality. Associations remained consistent when norharman concentrations were analyzed as binary variables (split by median). Urinary harman concentration seemed to show a negative association with cancer mortality. No definitive evidence was found of an association between other HAA and mortality. Urinary norharman concentration is associated with all-cause mortality and CVD mortality in adults.

show abstract

Fluoranthene determination based on a rapid and sensitive syringe extraction and solid‐phase fluorescence technique

Shen,

Li,

et al. 2023

Luminescence

View full text Add to dashboard Cite

A rapid and sensitive strategy was proposed for fluoranthene (FL) detection, which is polycyclic aromatic hydrocarbons (PAHs), in water samples. In this work, syringe SPE combined with solid‐phase fluorescence spectrometry was used to determine FL in PAHs polluted environmental samples. The fluorescent signals were directly monitored on the membrane surface after FL was enriched by syringe SPE. Under the optimal conditions, the proposed method showed a linear relationship in the concentration range of 2 – 50 μg/L with a correlation coefficient (R2) of 0.998, and the limit of detection (LOD) was 0.143 μg/L. The recoveries varied from 93.47 % to 109.81 % in the actual samples, with the relative standard deviations (n = 3) ranging from 2.06 to 6.32 %. According to the results, the established method can be applied in the field of rapid detection since it is fast, simple, portable, high sensitivity, and has strong anti‐interference.

show abstract

Changes in cardiac proteome and metabolome following exposure to the PAHs retene and fluoranthene and their mixture in developing rainbow trout alevins

Cited by 13 publications

References 76 publications

Endogenous AhR agonist FICZ accumulates in rainbow trout (Oncorhynchus mykiss) alevins exposed to a mixture of two PAHs, retene and fluoranthene

Endogenous AhR agonist FICZ accumulates in rainbow trout (Oncorhynchus mykiss) alevins exposed to a mixture of two PAHs, retene and fluoranthene

Association Between Urinary Heterocyclic Aromatic Amines and Mortality in Adults

Fluoranthene determination based on a rapid and sensitive syringe extraction and solid‐phase fluorescence technique

Contact Info

Product

Resources

About