Changes in Cellular Growth in Newborn Rats and Rabbits Exposed to Photoirradiation

Wu, Paul Y K; Yoshida, Takashi

doi:10.1159/000240726

Cited by 6 publications

(1 citation statement)

References 8 publications

(8 reference statements)

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“…Investigations of the non‐clinical adverse effects of intermittent versus continuous phototherapy have not been conclusive. Wu and Yoshida 7 reported on growth retardation in newborn rats and rabbits after continuous phototherapy compared to intermittent. On the other hand, Santella and co‐workers 8 observed more DNA damage in cells exposed to intermittent phototherapy.…”

Section: Introductionmentioning

confidence: 99%

Bilirubin‐induced cell death during continuous and intermittent phototherapy and in the dark

Roll

2005

Acta Paediatrica

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Aim: To compare continuous and intermittent light exposure in the presence of bilirubin with respect to cellular damage. Furthermore, it was of interest to characterize the nature of cellular toxicity of bilirubin in the dark. Method: A murine lymphoma cell line, L5178Y‐R (LY‐R), was exposed to solutions of bilirubin (160 μM) supplemented with human serum albumin (200 μM) and irradiated with phototherapy light (Philips 20W/52) at a constant total dose of approximately 500 kJ/m2. The irradiation was given either as intermittent or continuous treatment with light of variable irradiance. The three lower irradiance levels were clinically relevant. Cells treated with bilirubin were also kept in the dark for various periods of time. Cell toxicity was determined by measuring apoptosis and necrosis. Apoptosis was measured by terminal deoxynucleotide transferase and propidium iodide staining assay, while trypan blue assay was used for detection of necrosis. Results: There was no difference (n=6, p>0.05) between continuous and intermittent irradiation in the induction of early and late apoptotic cell death. Necrosis was more pronounced after intermittent treatment. Bilirubin dark toxicity was observed and classified as both apoptotic and necrotic. Conclusion: Continuous and intermittent light exposure caused the same degree of apoptotic cell death, while the cells underwent more necrotic death after intermittent exposure. Bilirubin was cytotoxic in the dark by both cell death mechanisms.

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Section: Introductionmentioning

confidence: 99%

Bilirubin‐induced cell death during continuous and intermittent phototherapy and in the dark

Roll

2005

Acta Paediatrica

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Identification of hydrogen peroxide as a photoproduct toxic to human cells in tissue-culture medium irradiated with “daylight” fluorescent light

1978

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Hydrogen peroxide, lethal for human cells, is produced in Dulbecco's modified Eagle's tissue culture medium when exposed to "daylight" fluorescent light. Addition of pure H2O2 and use of the enzyme catalase demonstrate that about 40% of the toxicity in irradiated medium is due to generated peroxide. Riboflavin and tryptophan, or riboflavin and tyrosine, are the components necessary for formation of lethal levels of H2O2 during light exposure.

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Phototherapy for Neonatal Hyperbilirubinemia: Efficacy, Mechanism and Toxicity*

Brown

McDonagh

1980

Advances in Pediatrics

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Changes in Cellular Growth in Newborn Rats and Rabbits Exposed to Photoirradiation

Cited by 6 publications

References 8 publications

Bilirubin‐induced cell death during continuous and intermittent phototherapy and in the dark

Bilirubin‐induced cell death during continuous and intermittent phototherapy and in the dark

Identification of hydrogen peroxide as a photoproduct toxic to human cells in tissue-culture medium irradiated with “daylight” fluorescent light

Phototherapy for Neonatal Hyperbilirubinemia: Efficacy, Mechanism and Toxicity*

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