Changes in Chromatin Structure along Aging

Moraes, Alberto da Silva

doi:10.4172/2167-7182.1000157

Cited by 3 publications

(2 citation statements)

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“…Therefore, AHL15 may control the ageing pathway by inducing changes in higher‐order chromatin organization that lead to repression of SPL genes, GA 3 biosynthesis, and other pathways. In animals, the contribution of higher‐order chromatin organization to ageing processes has been well documented (Moraes, 2014; Chandra & Kirschner, 2016). However, the actual involvement of higher‐order chromatin organization in the juvenile‐to‐adult transition in plants remains to be investigated.…”

Section: Discussionmentioning

confidence: 99%

miR156‐independent repression of the ageing pathway by longevity‐promoting AHL proteins in Arabidopsis

et al. 2022

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Summary Plants age by developmental phase changes. In Arabidopsis, the juvenile to adult vegetative phase change (VPC) is marked by clear heteroblastic changes in leaves. VPC and the subsequent vegetative to reproductive phase change are promoted by SQUAMOSA PROMOTOR BINDING PROTEIN‐LIKE (SPL) transcription factors and repressed by miR156/157 targeting SPL transcripts. By genetic, phenotypic, and gene expression analyses, we studied the role of the longevity‐promoting AT‐HOOK MOTIF NUCLEAR LOCALIZED 15 (AHL15) and family members in SPL‐driven plant ageing. Arabidopsis ahl loss‐of‐function mutants showed accelerated VPC and flowering, whereas AHL15 overexpression delayed these phase changes. Expression analysis and tissue‐specific AHL15 overexpression revealed that AHL15 affects VPC and flowering time directly through its expression in the shoot apical meristem and young leaves, and that AHL15 represses SPL2/9/13/15 gene expression in a miR156/157‐independent manner. The juvenile traits of spl loss‐of‐function mutants appeared to depend on enhanced expression of the AHL15 gene, whereas SPL activity prevented vegetative growth from axillary meristem by repressing AHL15 expression. Our results place AHL15 and close family members together with SPLs in a reciprocal regulatory feedback loop that modulates VPC, flowering time, and axillary meristem development in response to both internal and external signals.

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Section: Discussionmentioning

confidence: 99%

miR156‐independent repression of the ageing pathway by longevity‐promoting AHL proteins in Arabidopsis

et al. 2022

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“…In animals, the contribution of higher-order chromatin organization to ageing processes has been well documented (Moraes, 2014; Chandra and Kirschner, 2016; Gorbunova and Seluanov, 2016). In contrast, the role of global organization of chromatin architecture in plant Ageing is not described yet.…”

Section: Discussionmentioning

confidence: 99%

The effect of short-term activation of AHL15 on long-term plant developmental change and transcriptome profile

Karami

Henkel

Offringa

2022

Preprint

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We have previously documented that overexpression of the Arabidopsis nuclear protein AHL15 leads to reprogramming of somatic cells to embryonic cells (Karami et al., 2021) and to suppression of plant ageing (Karami et al., 2020). Here we show that transient (4 hours) activation of overexpressed AHL15-GR in Arabidopsis seedlings has long-term effects on plant development. RNA sequencing analysis detected an extensive reprogramming of the transcriptome 4 hours after AHL15-GR activation, with respectively 540 and 1107 genes showing more than 2-fold up- and down-regulation. AHL15 seemed to act in a transcription level-dependent manner, activating predominantly low expressed genes and repressing mostly highly expressed genes. Rapid decondensation of heterochromatin was observed after AHL15 activation in leaf primordia and axillary meristems, indicating that the global reprogramming of the transcriptome by transient activation of this AT-Hook domain protein might be caused by extensive modulation of the chromatin configuration. We also found that co-activated or co-repressed genes were often physically linked in small chromosomal clusters, which is in line with regulation at the chromatin level.

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