Changes in dietary fiber intake in mice reveal associations between colonic mucin <i>O</i>-glycosylation and specific gut bacteria

Gamage, Hasinika K. A. H.; Chong, Raymond W. W.; Bucio-Noble, Daniel; Kautto, Liisa; Hardikar, Anandwardhan A.; Ball, Malcolm S.; Molloy, Mark P.; Packer, Nicolle H.; Paulsen, Ian T.

doi:10.1080/19490976.2020.1802209

Cited by 32 publications

(37 citation statements)

References 64 publications

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“…Dietary fiber deprivation and a western-style diet have been shown to lead to the extinction of specific bacteria in mice [ 38 , 39 ]. Specifically, dietary fiber deprivation induces reduction of Bacteroidetes S24-7 family , Bifidobacterium , and expansion of Ruminococcaceae .…”

Section: Discussionmentioning

confidence: 99%

“…FF dietary fiber deprivation, FF + xylan, dietary fiber deprivation + xylan. C cecum, PC proximal colon, MC middle colon, DC distal colon, F feces Dietary fiber deprivation and a western-style diet have been shown to lead to the extinction of specific bacteria in mice [38,39]. Specifically, dietary fiber deprivation induces reduction of Bacteroidetes S24-7 family, Bifidobacterium, and expansion of Ruminococcaceae.…”

Section: Discussionmentioning

confidence: 99%

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Xylan alleviates dietary fiber deprivation-induced dysbiosis by selectively promoting Bifidobacterium pseudocatenulatum in pigs

Wang

Bai

et al. 2021

Microbiome

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Background Low dietary fiber intake has been shown to disturb the gut microbiome community, damage the mucus barrier, and promote pathogen susceptibility. However, little is known about the temporal response of the gut microbiome to dietary fiber deprivation and the recovery induced by dietary fiber inclusion in pigs. Objective In the present study, temporal responses of ileal and fecal microbiota to dietary fiber deprivation were profiled using an ileum cannulated growing pig model. In addition, the potential of dietary-resistant starch, β-glucan, and xylan to alleviate gut dysbiosis throughout the gastrointestinal tract, as well as its possible mechanisms were investigated. Methods Six cannulated growing pigs were fed a fiber deprivation diet for 35 days. Ileal digesta and feces were collected at days 0, 7, 21, and 35 for 16S rRNA sequencing and short-chain fatty acid (SCFA) determination. Another twenty-four healthy growing pigs were assigned to one of four dietary treatments including (1) fiber-free diet, (2) resistant starch diet, (3) β-glucan diet, and (4) xylan diet. These twenty-four pigs were fed a corresponding diet for 35 days and slaughtered. Gut microbiome and SCFA concentration were profiled along the gastrointestinal tract. Results Dietary fiber deprivation-induced consistent microbiota extinction, mainly Bifidobacterium and Lactobacillus, and decreased SCFA concentrations in both ileum and feces. The community structure partially recovered at day 35 compared with baseline while SCFA concentrations remained low. Xylan supplementation alleviated gut dysbiosis by selectively promoting Bifidobacterium pseudocatenulatum within the large intestine. SCFA concentration increased significantly after xylan supplementation and exhibited a positive association with B. pseudocatenulatum abundance. An elevated abundance of xylan degradation-related enzyme genes was also observed in the gut microbiome after xylan supplementation. In vitro growth assay further verified the xylan utilization capacity of B. pseudocatenulatum. Conclusions Dietary fiber deprivation could induce probiotic extinction and loss of the SCFA production while potential pathogen was promoted. Xylan intervention could partially restore dietary fiber deprivation-induced gut dysbiosis through selectively promoting B. pseudocatenulatum and therefore normalizing the gut environment. These findings collectively provide evidence that dietary fiber-driven microbiota metabolism bridges the interplay between microbiome and gut health.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Xylan alleviates dietary fiber deprivation-induced dysbiosis by selectively promoting Bifidobacterium pseudocatenulatum in pigs

Wang

Bai

et al. 2021

Microbiome

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“…Different Bacteroides species were recovered only from T1D children, namely B. graminisolvens , B. cellulosilyticus , B. finegoldii , B. stercoris , B. eggerthii and B. fragilis . Diet can modify the composition of the gut microbiota [ 54 , 55 , 56 , 57 ]. Bacteroides species are particularly modulated by the accessibility and abundance of dietary fiber [ 55 , 58 , 59 ], for example arabinoxylans that are naturally found in all major cereal grains increases the abundance of B. cellulosilyticus but not of B. ovatus [ 55 , 60 ].…”

Section: Discussionmentioning

confidence: 99%

“…The ability to break mucin, evidenced by amidoblack staining, was confirmed for all tested strains. Bacteroides species are recognized by their ability to degrade dietary fiber and host intestinal mucin [ 57 , 71 ]. Taking this into account, and knowing that hyperglycemia affects the intestinal mucus layer allowing a diminished microbiota-epithelial distance [ 72 ], P. dorei , P. vulgatus and other Bacteroidetes members in T1D gut may contribute to a disturbed mucus layer, therefore facilitating bacterial infiltration.…”

Section: Discussionmentioning

confidence: 99%

Insights from Bacteroides Species in Children with Type 1 Diabetes

Matos

Calha

et al. 2021

Microorganisms

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In our previous study the enrichment of the intestinal proteome of type 1 diabetes (T1D) children with Bacteroides proteins was observed, which led us to our current study that aimed to isolate and characterize Bacteroides species from fecal samples of T1D and control children. Repetitive sequence-based PCR (rep-PCR) was used for typing the isolated Bacteroides species. The antibiotic susceptibility and mucinolytic activity of the isolates was determined. The quantification of specific bacterial groups in the fecal samples was determined by qPCR. The ability to adhere and invade the human colonic cell line HT29-MTX-E12 of strains of P. dorei, B. uniformis and P. distasonis was determined and their whole genome sequencing was performed. The results showed similar numbers of Bacteroides species in T1D and control samples, but unique Bacteroides species and a higher recovery of P. distasonis from T1D samples was observed. Rep-PCR grouped the different Bacteroides species, but no discrimination by origin was achieved. T1D children showed a significant increase in Proteobacteria and a depletion in Lactobacillus sp. All tested P. dorei, B. uniformis and P. distasonis were able to adhere to HT29-MTX-E12 cells but significant differences (p < 0.05) in the ability to invade was observed. The highest ability to invade was exhibited by P. distasonis PtF D14MH1 and P. dorei PtFD16P1, while B. uniformis strains were unable to invade. The damage to tight junctions was also observed. The presence of Lactobacillus sp. inhibited the invasion ability of P. distasonis PtF D14MH1 but not P. dorei PtFD16P1. Sequences of agonist peptides of the human natural preproinsulin and the insulin B chain insB:9-23 peptide mimics were identified. The results reported in our study stresses the continued efforts required to clarify the link between T1D and gut microbiota.

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“…This makes mucin lose its stability. The decreased Bacteroides and Faecalibacterium prausnitzii also failed to promote the differentiation of goblet cells, and they could not regulate the glycosylation of the mucosa ( Figure 2 ) ( 53 – 55 ).…”

Section: Interaction Between Commensal Bacteria and The Intestinal Barrier In Ibdmentioning

confidence: 99%

Interaction Between Commensal Bacteria, Immune Response and the Intestinal Barrier in Inflammatory Bowel Disease

et al. 2021

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In inflammatory bowel disease (IBD), intestinal mucosa cell and intestinal epithelial cell are severely damaged, and then their susceptibility to bacteria increases, so many commensal bacteria become pathogenic. The pathogenic commensal bacteria can stimulate a series of compensatory immune responses in the intestine. However, the immune response prevents the intestinal tract from restoring homeostasis, which in turn produces an indispensable inflammatory response. On the contrary, in IBD, the fierce inflammatory response contributes to the development of IBD. However, the effect of commensal bacteria on inflammation in IBD has not been clearly studied. Therefore, we further summarize the changes brought about by the changes of commensal bacteria to the inflammation of the intestines and their mutual influence. This article reviews the protective mechanism of commensal bacteria in healthy people and the mechanism of commensal bacteria and immune response to the destruction of the intestinal barrier when IBD occurs. The treatment and prevention of IBD are also briefly summarized.

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Changes in dietary fiber intake in mice reveal associations between colonic mucin O-glycosylation and specific gut bacteria

Cited by 32 publications

References 64 publications

Xylan alleviates dietary fiber deprivation-induced dysbiosis by selectively promoting Bifidobacterium pseudocatenulatum in pigs

Xylan alleviates dietary fiber deprivation-induced dysbiosis by selectively promoting Bifidobacterium pseudocatenulatum in pigs

Insights from Bacteroides Species in Children with Type 1 Diabetes

Interaction Between Commensal Bacteria, Immune Response and the Intestinal Barrier in Inflammatory Bowel Disease

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