Changes in Erythrocyte Membrane Ouabain-Sensitive Adenosine Triphosphatase after Renal Transplantation

Cole, C.; Maletz, R.

doi:10.1042/cs0480239

Cited by 10 publications

(8 citation statements)

References 4 publications

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“…The present results obtained by the 86Rb uptake method are consistent with this finding and suggest that a similar alteration of Na+, K'-ATPase activity occurs in patients with ARF. In patients with previous CRF treated by renal transplantation, Cole and Maletz [4] and Cole et al [5] reported increased Na+, K+-ATPase activity, which con trasts with our results which did not show any difference in 86Rb uptake between these patients and paired controls.…”

Section: Discussionsupporting

confidence: 79%

“…Dransfield and Greff [6] reported that prednisone could alter in vitro Na% K -ATPase activity in RBC. But this does not provide an explanation for the discrepancy between our results and those of Cole and Maletz [4] and Cole et al [5] whose patients were also taking prednisone and azathio prine.…”

Section: Discussioncontrasting

confidence: 55%

“…Furthermore, Cole et al [3] have shown that plasma from uraemic patients with elevated intra-RBC sodium concentrations inhibits Na+, K^-ATPase/activity in the membranes of normal RBC. Later on, Cole [2] reported a decreased Na+, K+-ATPase activity in the RBC mem branes of patients with chronic renal disease and with nor mal intracellular sodium concentrations, and Cole and Maletz [4] reported a higher Na+, K -A TPase activity in RBC of patients after renal transplantation than in normal controls.…”

Section: Introductionmentioning

confidence: 99%

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Cation Transport in Erythrocytes of Patients with Renal Failure

Zannad¹,

Royer²,

Kessler³

et al. 1982

Nephron

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Na⁺, K⁺-ATPase activity in erythrocytes (RBC) has been assessed indirectly by the ⁸⁶Rb uptake method in 10 patients with acute renal failure (ARF), 11 patients with chronic renal failure (CRF) on haemodialysis and 13patients treated by kidney transplantation, and compared to age-matched healthy controls. In each patients, the sensitivity of ⁸⁶Rb uptake to digoxin (IC₅₀) has been measured. Na⁺, K⁺-ATPase activity was decreased in patients with ARF and in patients with CRF but not in transplanted patients. IC₅₀ was lowered in transplanted patients. The implications of these findings are discussed in terms of possible mechanisms of the changes in Na⁺, K⁺-ATPase activity in RBC of patients with renal failure.

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Section: Discussionsupporting

confidence: 79%

Section: Discussioncontrasting

confidence: 55%

Section: Introductionmentioning

confidence: 99%

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Cation Transport in Erythrocytes of Patients with Renal Failure

Zannad¹,

Royer²,

Kessler³

et al. 1982

Nephron

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“…It has been reported that erythrocyte membrane Na,K-ATPase activity is supranormal in renal transplant patients [24], and that erythrocyte Na content is normal or low [13,25], Ion flux studies suggest that Na,K pump function in transplanted patients is normal or increased [5,25]. In the present experiments, erythrocytes from these patients had normal membrane transport, ouabain binding properties and intracellular sodium concentra tions.…”

Section: Patients With Functional Transplantssupporting

confidence: 51%

Effects of Dialysis and Transplantation on Red Cell Na Pump Function in Renal Failure

Fervenza

Hendry

Ellory

1989

Nephron

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Ion pumping by the erythrocyte Na, K-ATPase has been measured using ouabain-sensitive ⁸⁶Rb flux in 11 non-dialysed patients with chronic renal failure (CRF), 13 patients on haemodialysis (HD), 13 patients on peritoneal dialysis (CAPD) and 15 patients with functional transplants (FT). Flux measurements were performed in plasma and simultaneous estimates of specific ³H-ouabain binding were made. The results indicate that, compared to normal controls, NaK pump flux was reduced by 21% in CRF (p < 0.01), 30% in HD (p < 0.01), 15% in CAPD (p < 0.02), and was normal in FT. Mean specific ouabain binding sites per cell ( ± SEM) were; controls 366 ± 16; CRF, 290 ± 16; HD, 344 ± 17; CAPD, 321 ± 18; FT, 345 ± 26. Calculation of mean turnover rate per pump site indicated that patients on HD showed a 30% reduction compared to controls (influx 55 K ions/s versus 79 K ions/s, p < 0.01). Cross-incubation experiments suggest that the lowered pump flux seen in the CRF and HD groups was due to plasma factors. This work shows that erythrocyte NaK pump number is reduced in CRF, while patients on maintenance HD have normal pump numbers per erythrocyte but reduced pump turnover.

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“…Cole and Maletz (37) reported decreased erythrocyte sodium and increased Na-K ATPase in renal transplant recipients, and observed that the changes were the obverse of those reported in uremia. In light of the findings presented here, it appears likely that the findings described in transplant recipients were, in large part, due to the administration of prednisone in those patients.…”

Section: Resultsmentioning

confidence: 91%

Glucocorticoid-induced alterations in the sodium potassium pump of the human erythrocyte.

Kaji¹,

Thakkar²,

Kahn³

1981

J. Clin. Invest.

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A B S T R A C T To evaluate the effects of glucocorticoids on the Na-K pump in human subjects, we evaluated the intracellular sodium and potassium, 42K influx across and the [3H]ouabain binding to cell membranes of intact human erythrocytes from a group of subjects taking glucocorticoids and a group of normal subjects. Intracellular sodium concentration was lower (7.2±0.4 vs. 10.9+0.2 mmol/liter cell water) and intracellular potassium concentration higher (149.8± 1.5 vs. 137.2±1.2 mmol/liter cell water) in erythrocytes from steroid-treated patients. In spite of a significantly decreased intracellular sodium which normally diminishes ouabain-sensitive 42K influx, the ouabain-sensitive K influx was unchanged in erythrocytes from the steroid-treated group. Maximum [3H]ouabain binding was markedly higher in the steroid-treated group (835±44 vs. 449±11 sites/cell). There was close linear correlation between [3H]ouabain binding and inhibition of K pump, suggesting the specificity of ouabain binding to Na-K pump sites on the cell membrane. Association kinetics for ouabain were similar in the two groups despite the marked difference in the amount of [3H]ouabain binding. External potassium concentration required for half-maximum ouabain-sensitive K influx was identical in the two groups. Thus, the additional Na-K pump sites in the steroid-treated group were qualitatively similar to those in normals. These results suggest that administration of glucocorticoids leads to an increase in the number of Na-K pump sites. The increase in the number of Na-K pump sites may explain the low levels of intracellular sodium and higher cell potassium observed in steroid-treated subjects.

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Changes in Erythrocyte Membrane Ouabain-Sensitive Adenosine Triphosphatase after Renal Transplantation

Cited by 10 publications

References 4 publications

Cation Transport in Erythrocytes of Patients with Renal Failure

Cation Transport in Erythrocytes of Patients with Renal Failure

Effects of Dialysis and Transplantation on Red Cell Na Pump Function in Renal Failure

Glucocorticoid-induced alterations in the sodium potassium pump of the human erythrocyte.

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