Changes in expression of calbindin 28 kDa in the small intestine of red kidney bean (Phaseolus vulgaris) lectin-treated suckling piglets

Zacharko-Siembida, Anna; Piedra, J.L. Valverde; Arciszewski, Marcin B.

doi:10.2478/pjvs-2013-0029

Cited by 4 publications

(3 citation statements)

References 33 publications

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“…All images were obtained with a digital color camera (DP-70, Olympus) using the Cell^M image system software (Olympus). In all segments of the small and large intestines of the control and experimental animals, the numbers of NEC IR for serotonin, GLP1, or somatostatin were assessed by cell counting according to a protocol described elsewhere (39). Briefly, cell counts were performed in 10 mm long section of non-interrupted and not damaged intestinal mucosa (at least 5 slides from intestines of each animal from every group).…”

Section: Methodsmentioning

confidence: 99%

Expression of serotonin, somatostatin, and glucagon-like peptide 1 (GLP1) in the intestinal neuroendocrine cells of pigs fed with population rye type and hybrid rye type grains

Zacharko-Siembida¹,

Arciszewski²,

Piedra³

et al. 2019

Medycyna Weterynaryjna

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Neuroendocrine cells (NEC) are a cell population in the gastrointestinal tract that plays a role in the regulation of the digestion process, satiety and nutrient homeostasis. NE cells express a variety of bioactive hormones that can undergo changes in response to different luminal stimuli, including multiple components, which are present in the diet. In recent years, a modern (hybrid) type of rye grain has been introduced to feed industry. The goal of the present study was to determine immunohistochemically whether the feeding of the pigs with population and hybrid rye grains may evoke adverse changes in the small and large intestines in terms of the expression of serotonin, glucagon-like peptide 1 (GLP1) and somatostatin. Feeding animals with population and hybrid rye grains resulted in a slight increase in serotonin-positive NE cells in the small intestine (but not in the large intestine). After feeding animals with population rye (but not with hybrid rye) grains, there was a decrease in the small intestine GLP1-immunoreactive NE cells was found. No changes in the expression of GLP1 were found in the large intestine of experimental animals. The numbers of somatostatin-IR NEC in the small and large intestines were not affected by feeding with either population or hybrid rye grains. In conclusion, we found that feeding pigs with hybrid and population rye grains started adaptive changes in NEC. However, those changes were not profound, which allows us to speculate that adverse effects of these rye grains have a minor (if any) impact on the gut hormone balance (and indirectly on the health status) of animals.

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Section: Methodsmentioning

confidence: 99%

Expression of serotonin, somatostatin, and glucagon-like peptide 1 (GLP1) in the intestinal neuroendocrine cells of pigs fed with population rye type and hybrid rye type grains

Zacharko-Siembida¹,

Arciszewski²,

Piedra³

et al. 2019

Medycyna Weterynaryjna

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“…Control sections showed no detectable labelling. Previously described counting protocol was applied in the image analysis (30). Briefly, in the sections with no visible damage of mucosa, all EC were identified and counted.…”

Section: Methodsmentioning

confidence: 99%

“…The distribution and number of rat EEC were locally changed dependently on the diet type and the presence of the gut microflora (27). Recently, it has been also found that the number of calbindin-containing EEC increases in suckling pigs treated with lectin (30).…”

Section: Introductionmentioning

confidence: 99%

Red kidney bean (Phaseolus vulgaris) lectin stimulation increases the number of enterochromaffin cells in the small intestine of suckling piglets

Zacharko-Siembida¹,

Piedra

Strzałka³

et al. 2014

Bulletin of the Veterinary Institute in Pulawy

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The quantities and distribution patterns of serotonin-immunoreactive (serotonin-IR) enterochromaffin cells (EC) were studied immunohistochemically in the small intestine of suckling piglets stimulated with red kidney bean lectin, and in nonstimulated, control animals. The co-expression patterns of serotonin with somatostatin (SOM) or corticotropin releasing-factor (CRF) were also studied. After the lectin treatment, the increased numbers of EC were noted in the duodenum of experimental animals. Lectin stimulation did not change the proportions of EC in the jejunum and ileum. In the duodenal epithelium of the lectin-stimulated piglets, the vast majority of serotonin-IR EC were distributed at the basis of crypts. After the lectin administration, the proportions of serotonin-IR/SOM-IR EC were statistically similar in all sections of the small intestine. No upregulation of CRF was found in duodenal, jejunal, and ileal EC of lectin-treated animals. The findings demonstrated that red kidney bean lectin increased the serotonin reservoir in the duodenum, and thus may be an effective stimulant of the gut maturation in suckling mammals.

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Neurochemical characteristics of calbindin-like immunoreactive coeliac-cranial mesenteric ganglion complex (CCMG) neurons supplying the pre-pyloric region of the porcine stomach

et al. 2018

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Changes in expression of calbindin 28 kDa in the small intestine of red kidney bean (Phaseolus vulgaris) lectin-treated suckling piglets

Cited by 4 publications

References 33 publications

Expression of serotonin, somatostatin, and glucagon-like peptide 1 (GLP1) in the intestinal neuroendocrine cells of pigs fed with population rye type and hybrid rye type grains

Expression of serotonin, somatostatin, and glucagon-like peptide 1 (GLP1) in the intestinal neuroendocrine cells of pigs fed with population rye type and hybrid rye type grains

Red kidney bean (Phaseolus vulgaris) lectin stimulation increases the number of enterochromaffin cells in the small intestine of suckling piglets

Neurochemical characteristics of calbindin-like immunoreactive coeliac-cranial mesenteric ganglion complex (CCMG) neurons supplying the pre-pyloric region of the porcine stomach

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