Changes in expression of mRNAs in the gerbil hippocampus following transient forebrain ischemia

Identification of Potential Stroke Targets by Lentiviral Vector Mediated Overexpression of HIF-1α and HIF-2α in a Primary Neuronal Model of Hypoxia

“…Interpretation of differential gene expression data from such models has proven difficult, due to the genetic heterogeneity and diversity of the infarct pathophysiology between models together with spatial and temporal variations Sharp et al, 2000;Tang et al, 2002). The majority of animal studies, due to their nature, have investigated expression changes at relatively late timepoints following the onset of ischemia and this may have prevented the identification of early and potentially critical postischemic changes in gene expression (Kobayashi et al, 2000). Furthermore, expression changes in neuronal cell types are subtle and therefore it is critical that highly sensitive methods of detection are employed.…”