Changes in fatty acids, blood biochemistry and mRNA expressions of genes involved in polyunsaturated fatty acid metabolism in brown trout (<i>Salmo trutta</i>) during starvation and refeeding

Arslan, Gökhan; Bayır, Mehtap; Yağanoğlu, Aycan Mutlu; Bayır, Abdulkadir

doi:10.1111/are.14908

Cited by 14 publications

(13 citation statements)

References 76 publications

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“…Contrary to the starvation-induced enhancement of lipolysis in the nonmuscular tissues of some teleosts [ 5 , 49 – 51 ], including our previous data in the gut of starved S. hasta [ 5 ], the transcript levels of lpl and cpt1a were consistently downregulated and decreased to their lowest abundance after 14 days of fasting ( Figure 5 ). These responses of lpl and cpt1a to starvation suggested that the biological processes involved in lipid hydrolysis and fatty acid β -oxidation were suppressed in S. hasta muscle.…”

Section: Discussioncontrasting

confidence: 61%

“…Considering the vital role of fas and scd1 in lipid biosynthesis, the changing trends observed herein indicated that two weeks of food deprivation decreased intramuscular lipid accumulation by transcriptionally impeding fatty acid de novo synthesis in the muscle tissues of S. hasta, especially monounsaturated fatty acids (MUFAs). Contrary to the starvation-induced enhancement of lipolysis in the nonmuscular tissues of some teleosts [5,[49][50][51], including our previous data in the gut of starved S. hasta [5], the transcript levels of lpl and cpt1a were consistently downregulated and decreased to their lowest abundance after 14 days of fasting (Figure 5). These responses of lpl and cpt1a to starvation suggested that the biological processes involved in lipid hydrolysis and fatty acid β-oxidation were suppressed in S. hasta muscle.…”

Section: Effects Of Starvation On the Expression Of Lipidcontrasting

confidence: 71%

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Starvation Affects the Muscular Morphology, Antioxidant Enzyme Activity, Expression of Lipid Metabolism-Related Genes, and Transcriptomic Profile of Javelin Goby (Synechogobius hasta)

Chen

Xu²,

Cui³

et al. 2022

Aquaculture Nutrition

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Fish in natural and cultivated environments can be challenged by starvation. However, inducing starvation in a controlled manner cannot only reduce feed consumption but also reduces aquatic eutrophication and even improves farmed fish quality. This study investigated the effects of starvation on the muscular function, morphology, and regulatory signaling in javelin goby (Synechogobius hasta) by evaluating the biochemical, histological, antioxidant, and transcriptional changes in the musculature of S. hasta subjected to 3, 7, and 14 days fasting. The muscle glycogen and triglyceride levels in S. hasta were gradually reduced under starvation, reaching their lowest at the end of the trial ( P < 0.05 ). The levels of glutathione and superoxide dismutase were significantly elevated after 3–7 days of starvation ( P < 0.05 ), but later returned to the level of the control group. The muscle of starved S. hasta developed structural abnormalities in some areas after 7 days of food deprivation, and more vacuolation and more atrophic myofibers were observed in 14-day fasted fish. The transcript levels of stearoyl-CoA desaturase 1 (scd1), the key gene involved in the biosynthesis of monounsaturated fatty acids, were markedly lower in the groups starved for 7 or more days ( P < 0.05 ). However, the relative expressions of genes associated with lipolysis were decreased in the fasting experiment ( P < 0 .05). Similar declines in the transcriptional response to starvation were found in muscle fatp1 and ppar γ abundance ( P < 0.05 ). Furthermore, the de novo transcriptome of muscle tissue from the control, 3-day and 14-day starved S. hasta generated 79,255 unigenes. The numbers of differentially expressed genes (DEGs) identified by pairwise comparisons among three groups were 3276, 7354, and 542, respectively. The enrichment analysis revealed that the DEGs were primarily involved in metabolism-related pathways, including ribosome, TCA pathway, and pyruvate metabolism. Moreover, the qRT-PCR results of 12 DEGs validated the expression trends observed in the RNA-seq data. Taken together, these findings demonstrated the specific phenotypical and molecular responses of muscular function and morphology in starved S. hasta, which may offer preliminary reference data for optimizing operational strategies incorporating fasting/refeeding cycles in aquaculture.

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Section: Discussioncontrasting

confidence: 61%

Section: Effects Of Starvation On the Expression Of Lipidcontrasting

confidence: 71%

Starvation Affects the Muscular Morphology, Antioxidant Enzyme Activity, Expression of Lipid Metabolism-Related Genes, and Transcriptomic Profile of Javelin Goby (Synechogobius hasta)

Chen

Xu²,

Cui³

et al. 2022

Aquaculture Nutrition

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show abstract

“…For these reasons, the potential relationship between food deprivation and changes in the lipid metabolites levels is unclear. Accordingly, there have been few studies conducted to evaluate how starvation regulates the expression levels of genes related to lipid metabolism in fish [ 14 , 16 ]. Onychostoma macrolepis is a national second-class protected animal in China, which has the dual value of protection and utilization.…”

Section: Introductionmentioning

confidence: 99%

Effects of Starvation and Refeeding on Growth, Digestion, Nonspecific Immunity and Lipid-Metabolism-Related Genes in Onychostoma macrolepis

Gou

Wang

Jin

et al. 2023

Animals

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The present research was conducted to assess the influences of starvation and refeeding on growth, nonspecific immunity and lipid metabolic adaptation in Onychostoma macrolepis. To date, there have been no similar reports in O. macrolepis. The fish were randomly assigned into two groups: control group (continuous feeding for six weeks) and starved–refed group (starvation for three weeks and then refeeding for three weeks). After three weeks of starvation, the results showed that the body weight (BW, 1.44 g), condition factor (CF, 1.17%), visceral index (VSI, 3.96%), hepatopancreas index (HSI, 0.93%) and intraperitoneal fat index (IPFI, 0.70%) of fish were significantly lower compared to the control group (BW, 5.72 g; CF, 1.85%; VSI, 6.35%; HSI, 2.04%; IPFI, 1.92%) (p < 0.05). After starvation, the serum triglyceride (TG, 0.83 mmol/L), total cholesterol (T-GHOL, 1.15 mmol/L), high-density lipoprotein (HDL, 1.13 mmol/L) and low-density lipoprotein (LDL, 0.46 mmol/L) concentrations were significantly lower than those in the control group (TG, 1.69 mmol/L; T-GHOL, 1.86 mmol/L; HDL, 1.62 mmol/L; LDL, 0.63 mmol/L) (p < 0.05). The activities of intestinal digestive enzymes (amylase, lipase and protease) in the starved-refed group were significantly lower than those in the control group after three weeks of starvation (p < 0.05). The highest activities of immune enzymes such as lysozyme (LZM), acid phosphate (ACP), alkaline phosphate (ALP), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX) and catalase (CAT) in the hepatopancreas were presented in the starved–refed group at second week, and significantly higher than those in the control group (p < 0.05). Meanwhile, starvation significantly improved intestinal immune enzymes activities (p < 0.05). the lowest TG contents and the highest expression levels of lipolysis genes including hormone-sensitive lipase (HSL) and carnitine palmitoyl transferase 1 isoform A (CPT-1A) appeared in the hepatopancreas, muscle and intraperitoneal fat after starvation, indicating the mobilization of fat reserves in these tissues (p < 0.05). After refeeding, the recovery of TG content might be mediated by the upregulation of the expression levels of lipogenesis genes such as sterol regulatory element binding protein 1 (SREBP1) and fatty acid synthase (FAS). Understanding the duration of physiological and metabolic changes in O. macrolepis and their reversibility or irreversibility to supplementary feeding response could provide valuable reference for the adaptability of O. macrolepis in large-scale culturing, proliferation and release.

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“…Many fish species experience natural periods of starvation in their habitat due to environmental evolution, seasonal food shortage, migration, transportation, food competition, reproduction and climate change [ 1 , 2 , 3 , 4 , 5 , 6 ]. In aquaculture, short-term starvation is considered as a strategy to control water quality, reduce handling stress, reduce disease-causing fish mortality, decrease feed costs, increase profitability, increase product quality and decrease flesh lipid content [ 4 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

An Integrated Bioinformatics Approach to Identify Network-Derived Hub Genes in Starving Zebrafish

Mortazavi

Ghaderi–Zefrehei

Dolatabady

et al. 2022

Animals

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The present study was aimed at identifying causative hub genes within modules formed by co-expression and protein–protein interaction (PPI) networks, followed by Bayesian network (BN) construction in the liver transcriptome of starved zebrafish. To this end, the GSE11107 and GSE112272 datasets from the GEO databases were downloaded and meta-analyzed using the MetaDE package, an add-on R package. Differentially expressed genes (DEGs) were identified based upon expression intensity N(µ = 0.2, σ2 = 0.4). Reconstruction of BNs was performed by the bnlearn R package on genes within modules using STRINGdb and CEMiTool. ndufs5 (shared among PPI, BN and COEX), rps26, rpl10, sdhc (shared between PPI and BN), ndufa6, ndufa10, ndufb8 (shared between PPI and COEX), skp1, atp5h, ndufb10, rpl5b, zgc:193613, zgc:123327, zgc:123178, wu:fc58f10, zgc:111986, wu:fc37b12, taldo1, wu:fb62f08, zgc:64133 and acp5a (shared between COEX and BN) were identified as causative hub genes affecting gene expression in the liver of starving zebrafish. Future work will shed light on using integrative analyses of miRNA and DNA microarrays simultaneously, and performing in silico and experimental validation of these hub-causative (CST) genes affecting starvation in zebrafish.

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Changes in fatty acids, blood biochemistry and mRNA expressions of genes involved in polyunsaturated fatty acid metabolism in brown trout (Salmo trutta) during starvation and refeeding

Cited by 14 publications

References 76 publications

Starvation Affects the Muscular Morphology, Antioxidant Enzyme Activity, Expression of Lipid Metabolism-Related Genes, and Transcriptomic Profile of Javelin Goby (Synechogobius hasta)

Starvation Affects the Muscular Morphology, Antioxidant Enzyme Activity, Expression of Lipid Metabolism-Related Genes, and Transcriptomic Profile of Javelin Goby (Synechogobius hasta)

Effects of Starvation and Refeeding on Growth, Digestion, Nonspecific Immunity and Lipid-Metabolism-Related Genes in Onychostoma macrolepis

An Integrated Bioinformatics Approach to Identify Network-Derived Hub Genes in Starving Zebrafish

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