Changes in glycoconjugate expression during early chick embryo development: A lectin‐binding study

Griffith, C.M.; Sanders, Esmond J.

doi:10.1002/ar.1092310212

Cited by 29 publications

(13 citation statements)

References 47 publications

(46 reference statements)

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“…This difference in staining probably reflects developmentally regulated changes in the distribution of cell surface oligosaccharides and our findings thus corroborate those of Griffith and Sanders (1991) and Nico et al (1998) in earlier developmental stages.…”

Section: Discussionsupporting

confidence: 78%

Lectin histochemistry of microvascular endothelium in chick and quail musculature

Naňka

Peumans

Damme

et al. 2001

Anatomy and Embryology

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The lectin binding pattern of muscular microvessels in chick, quail and chick/quail chimeras was analysed. Paraffin wax sections of muscles from embryonic and adult animals were used. The biotin-labelled lectins were detected by avidin-alkaline phosphatase complex. The following lectins bound to muscular microvessels including arterioles, capillaries and venules of both species: SNA-I (Sambucus nigra agglutinin), MAA (Maackia amurensis agglutinin), AIA (Artocarpus integrifolia agglutinin), VAA-I, VAA-II and VAA-III (Viscum album agglutinin I-III), WGA (wheat germ agglutinin), LEA (Lycopersicon esculentum agglutinin). Endomysium and basement membranes of muscle fibres were also stained to a variable extent and intensity. Only SNA-I stained almost exclusively the endothelium of blood vessels. WFA (Wisteria floribunda agglutinin) bound to the quail endothelium only. MPA (Maclura pomifera agglutinin) marked vessels in adult muscles of chick and quail, but embryonic vessels were stained in quail only. Our results show that lectin histochemistry is a useful tool for visualisation of microvasculature in avian species. In particular, WFA and MPA can be used to determine the origin of endothelia in chick/quail chimeras.

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Section: Discussionsupporting

confidence: 78%

Lectin histochemistry of microvascular endothelium in chick and quail musculature

Naňka

Peumans

Damme

et al. 2001

Anatomy and Embryology

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“…The use of lectins to probe specific glycans on cultured cell lines is well precedented (36,37). Lectins have enabled glycan visualization on tissue sections or whole-mount specimens at discrete time points in mouse (38), chick (39), and f ly (40) embryogenesis, as well as in the mature mouse thymus (41), rat endothelial vasculature (42), and human kidney (43). Further, lectins have been used to facilitate screening of Caenorhabditis elegans mutants with glycosylation defects (44,45).…”

Section: Imaging Glycans With Lectins and Antibodiesmentioning

confidence: 99%

Imaging the glycome

Laughlin¹,

Bertozzi

2009

Proc. Natl. Acad. Sci. U.S.A.

346

299

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“…The rapidity and complexity of the changes in carbohydrate residues during embryogenesis suggest that they play important roles for the development (3,5,14,26). During tooth development, Nakai et al (15) observed a moderate-to-strong binding of SBA in the stratum intermedium of well-differentiated ameloblasts.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of Soybean Agglutinin Binding Sites in Hyperplastic Dental Epithelium in Osteopetrotic (OP/OP) Mice.

Tajima

Yokose

Takenoya

et al. 1993

Acta Histochem. Cytochem

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In the present study, we examined SBA lectin binding sites and patterns in hyperplastic dental epithelium overlying the crowns of pathologically unerupted molar teeth from congenital osteopetrotic (OP/OP) mice, and compared our findings with the pattern seen in the normal littermates.The hyperplastic dental epithelium of OP/OP mice disclosed preferential and intense staining of SBA. The lectin binding sites were demonstrated on membrane of the epithelial cells that interconnected with superficial gingival epithelium. At ultrastructural level, SBA labeling was detected over the desmosomal complexes of the epithelial cells. In normal mice, the reducing dental epithelium, as well as junctional epithelial cells, likewise demonstrated the lectin staining, but to a significantly lesser extent. The results of this study, taken together with suggested property of SBA binding, indicate that the hyperplastic dental epithelium in OP/OP mice might overexpress sugar sequences with N-acetyl-D-galactosamine on their cell surface, and this may be attributed to a functional alteration or degradation of such cell surface, due secondary to the pathological aberrations residing in OP/OP mice.Osteopetrosis is an inherited disorder characterized by abnormally dense bones with a reduced medullary bone cavity (13). In osteopetrotic animals, the delayed or distorted tooth eruption has been shown to be coordinated with disturbance of bone resorption (17, 18), due to defective osteoclast differentiation and function (10 ,21). It has been suggested that early ankylosis, perhaps secondary to congenital reducion of bone resorption, is the major cause of the dental abnormalities (18). In a previous report of osteopetrotic (OP/OP) rats (17), the dental epithelium is not reduced and manifests a considerable degree of hyperplasia interconnecting with superficial gingival epithelium. However, little is known about the physiological significance of overgrowth of the dental epithelium, which is normally reduced tooth eruption, in osteopetrotic animals.Lectins are glycoproteins that bind to specific carbohydrate residues, and they provide useful information about cellular differentiation (4). Lectin staining has been shown to be a useful tool for characterization of epithelial differentiation (7,12,22), as well as developmental aspects of the tooth (1,15,16). Recently, Jowett et al. (8) demonstrated developmentally regulated lectin binding sites and patterns in murine molar teeth. These authors suggested that complex galactosamine residues are widely distributed within the tooth germ and that they show subtle tissue specificity.Taken together with aforementioned evidence, we selected soybean agglutinin (SBA), which is known to bind N-acetyl-D-galactosamine (GalNAc) (11), as a histochemical probe to evaluate the hyperplastic dental epithelium forming over the embedded molar teeth of OP/OP mice. Then we compared our findings with those on the binding sites in normal littermates. MATERIALS AND METHODS Preparation of tissue sectionsMandibles were obtai...

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Changes in glycoconjugate expression during early chick embryo development: A lectin‐binding study

Cited by 29 publications

References 47 publications

Lectin histochemistry of microvascular endothelium in chick and quail musculature

Lectin histochemistry of microvascular endothelium in chick and quail musculature

Imaging the glycome

Overexpression of Soybean Agglutinin Binding Sites in Hyperplastic Dental Epithelium in Osteopetrotic (OP/OP) Mice.

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