erates frequent defoliation in monoculture (Peterson et al., 1994) or in binary mixture with grass (Kim, 1996) Improved dry matter (DM) production of cool-season grass monoand is suitable for hay or pasture production in this cultures may result from either N fertilization or the addition of legumes such as kura clover (Trifolium ambiguum M. Bieb.) or birds-region Sheaffer et al., 1992). foot trefoil (Lotus corniculatus L.). Such improvements could affect Kura clover has been identified as a grass-compatible forage nutritive value and potential milk production from dairy cattle legume with excellent persistence in mechanically har-(Bos taurus). Laboratory estimates of forage nutritive value as well vested production systems (Kim, 1996; Zemenchik et al., as potential milk production per unit mass and area were compared 2001). However, there is limited information available for six levels of N fertilizer on Kentucky bluegrass (KBG; Poa praregarding laboratory estimates of forage quality or potensis L.), smooth bromegrass (SBG; Bromus inermis Leyss.), and tential milk production changes that may result by addorchardgrass (OG; Dactylis glomerata L.) in monoculture or with ing kura clover to cool-season grass swards. Peterson et either kura clover or birdsfoot trefoil in binary mixture with each al. (1994) reported that kura clover leaf proportion of grass. Experiments managed in a three-harvest system were conducted total harvested forage DM was positively correlated to from 1994 through 1996 near Arlington and Lancaster, WI. Either legume reduced neutral detergent fiber and acid detergent fiber and crude protein (CP) and inversely correlated to concenincreased crude protein when added to grass. These effects increased trations of acid detergent fiber (ADF) and neutral deterwith greater mixture legume DM proportions. Compared with grass gent fiber (NDF). Kim (1996) related harvest frequency monocultures averaged across years and locations and for any N rate, and height to legume composition of kura clover-grass mixtures had greater potential milk production per megagram of DM mixtures and kura clover monoculture but did not inand followed KBG Ͼ SBG Ͼ OG. Similarly, potential milk production clude a grass monoculture as one of the control treatper hectare for kura clover-grass mixtures exceeded that of monoculments. Zemenchik et al. (2001) estimated that kura clotures by at least 49% for KBG and 12% for SBG while birdsfoot trefoilver grown in binary mixtures with Kentucky bluegrass grass mixtures were greater by at least 28% for KBG and 20% for SBG.(KBG) resulted in total-season DM yields equivalent to Orchardgrass required maximum N rates to match milk production per 251 kg N ha Ϫ1 split-applied to that grass in monoculture.