Changes in
 cspL
 ,
 cspP
 , and
 cspC
 mRNA Abundance as a Function of Cold Shock and Growth Phase in
 Lactobacillus plantarum

Derzelle, Sylviane; Hallet, Bernard; Francis, Kevin P.; Ferain, Thierry; Delcour, Jean; Hols, Pascal

doi:10.1128/jb.182.18.5105-5113.2000

Cited by 59 publications

(58 citation statements)

References 49 publications

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“…4). Similar to certain CSPs of E. coli and Lactobacillus plantarum (Derzelle et al, 2000;Yamanaka & Inouye, 1997), the growth phase can affect CSP expression in B. bronchiseptica, since the T3 cspC transcript was found to accumulate strongly in stationary phase (data not shown).…”

Section: Discussionmentioning

confidence: 99%

Identification and regulation of cold-inducible factors of Bordetella bronchiseptica

et al. 2005

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The expression of bacterial cold-shock proteins (CSPs) is highly induced in response to cold shock, and some CSPs are essential for cells to resume growth at low temperature. Bordetella bronchiseptica encodes five CSPs (named CspA to CspE) with significant amino acid homology to CspA of Escherichia coli. In contrast to E. coli, the insertional knock-out of a single csp gene (cspB) strongly affected growth of B. bronchiseptica independent of temperature. In the case of three of the csp genes (cspA, cspB, cspC) more than one specific transcript could be detected. The net amount of cspA, cspB and cspC transcripts increased strongly after cold shock, while no such effect could be observed for cspD and cspE. The exposure to other stress conditions, including translation inhibitors, heat shock, osmotic stress and nutrient deprivation in the stationary phase, indicated that the csp genes are also responsive to these conditions. The coding regions of all of the cold-shock genes are preceded by a long non-translated upstream region (59-UTR). In the case of the cspB gene, a deletion of parts of this region led to a significant reduction of translation of the resulting truncated transcript, indicating a role of the 59-UTR in translational control. The cold-shock stimulon was investigated by 2D-PAGE and mass spectrometric characterization, leading to the identification of additional cold-inducible proteins (CIPs). Interestingly, two cold-shock genes (cspC and cspD) were found to be under the negative control of the BvgAS system, the main transcriptional regulator of Bordetella virulence genes. Moreover, a negative effect of slight overexpression of CspB, but not of the other CSPs, on the transcription of the adenylate cyclase toxin CyaA of Bordetella pertussis was observed, suggesting cross-talk between the CSP-mediated stress response stimulon and the Bordetella virulence regulon.

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Section: Discussionmentioning

confidence: 99%

Identification and regulation of cold-inducible factors of Bordetella bronchiseptica

et al. 2005

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“…plantarum NC8 (339,456). As mentioned earlier, CSPs are induced as a result of cold shock pretreatment, and they have been associated with the stabilization of mRNA (457). Three induced CSPs were previously identified in Lb.…”

Section: Stress Associated With Technological Productionmentioning

confidence: 99%

“…Three induced CSPs were previously identified in Lb. plantarum following cold shock treatment, namely, CspL, CspP, and CspC (457).…”

Section: Stress Associated With Technological Productionmentioning

confidence: 99%

Stress Physiology of Lactic Acid Bacteria

Papadimitriou

Alegría

Bron

et al. 2016

Microbiol Mol Biol Rev

539

404

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SUMMARYLactic acid bacteria (LAB) are important starter, commensal, or pathogenic microorganisms. The stress physiology of LAB has been studied in depth for over 2 decades, fueled mostly by the technological implications of LAB robustness in the food industry. Survival of probiotic LAB in the host and the potential relatedness of LAB virulence to their stress resilience have intensified interest in the field. Thus, a wealth of information concerning stress responses exists today for strains as diverse as starter (e.g.,Lactococcus lactis), probiotic (e.g., severalLactobacillusspp.), and pathogenic (e.g.,EnterococcusandStreptococcusspp.) LAB. Here we present the state of the art for LAB stress behavior. We describe the multitude of stresses that LAB are confronted with, and we present the experimental context used to study the stress responses of LAB, focusing on adaptation, habituation, and cross-protection as well as on self-induced multistress resistance in stationary phase, biofilms, and dormancy. We also consider stress responses at the population and single-cell levels. Subsequently, we concentrate on the stress defense mechanisms that have been reported to date, grouping them according to their direct participation in preserving cell energy, defending macromolecules, and protecting the cell envelope. Stress-induced responses of probiotic LAB and commensal/pathogenic LAB are highlighted separately due to the complexity of the peculiar multistress conditions to which these bacteria are subjected in their hosts. Induction of prophages under environmental stresses is then discussed. Finally, we present systems-based strategies to characterize the “stressome” of LAB and to engineer new food-related and probiotic LAB with improved stress tolerance.

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“…Total RNA was extracted from L. plantarum Lp80 by mechanical lysis with glass beads as described previously (5). Primer extension and sequencing reactions were performed by using nested primers pox4 and pox5 as described previously (13).…”

Section: Methodsmentioning

confidence: 99%

Characterization and Functional Analysis of thepoxBGene, Which Encodes Pyruvate Oxidase inLactobacillus plantarum

et al. 2004

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The pyruvate oxidase gene (poxB) from Lactobacillus plantarum Lp80 was cloned and characterized. Northern blot and primer extension analyses revealed that transcription of poxB is monocistronic and under the control of a vegetative promoter. poxB mRNA expression was strongly induced by aeration and was repressed by glucose. Moreover, Northern blotting performed at different stages of growth showed that poxB expression is maximal in the early stationary phase when glucose is exhausted. Primer extension and in vivo footprint analyses revealed that glucose repression of poxB is mediated by CcpA binding to the cre site identified in the promoter region. The functional role of the PoxB enzyme was studied by using gene overexpression and knockout in order to evaluate its implications for acetate production. Constitutive overproduction of PoxB in L. plantarum revealed the predominant role of pyruvate oxidase in the control of acetate production under aerobic conditions. The ⌬poxB mutant strain exhibited a moderate (20 to 25%) decrease in acetate production when it was grown on glucose as the carbon source, and residual pyruvate oxidase activity that was between 20 and 85% of the wild-type activity was observed with glucose limitation (0.2% glucose). In contrast, when the organism was grown on maltose, the poxB mutation resulted in a large (60 to 80%) decrease in acetate production. In agreement with the latter observation, the level of residual pyruvate oxidase activity with maltose limitation (0.2% maltose) was less than 10% of the wild-type level of activity.

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Changes in cspL , cspP , and cspC mRNA Abundance as a Function of Cold Shock and Growth Phase in Lactobacillus plantarum

Cited by 59 publications

References 49 publications

Identification and regulation of cold-inducible factors of Bordetella bronchiseptica

Identification and regulation of cold-inducible factors of Bordetella bronchiseptica

Stress Physiology of Lactic Acid Bacteria

Characterization and Functional Analysis of thepoxBGene, Which Encodes Pyruvate Oxidase inLactobacillus plantarum

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