Changes in in Vitro Methane Production and Fatty Acid Profiles in Response to Cakalang Fish Oil Supplementation

Sondakh, E.H.B.; Waani, M. R.; Kalele, Jerry A.D.

doi:10.5398/medpet.2017.40.3.188

Cited by 4 publications

(3 citation statements)

References 16 publications

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“…Approximately 200 mg samples (DM basis) were weighed into 100 mL glass syringes (Fortuna, Labortechnik, Germany) and filled with 30 mL rumen buffer fluid. Approximately 10 mL of medium was collected after 48 h incubation to determine pH, NH 3 concentration and single chain fatty acids (SCFAs) (Sondakh et al, 2017). Based on the proportions of SCFAs, methane production was estimated using the following equation (Widiawati & Thalib, 2007): CH 4 (mM)= (0.5 × acetate concentration) + (0.5 × butyrate concentration) -(0.25 × propionate concentration)…”

Section: In Vitro Assaymentioning

confidence: 99%

Forage-Yield and Nutrient Quality of New Brown Midrib (BMR) Mutant Lines of Sorghum

Wahyono

Indriatama

Sasongko

et al. 2023

Trop. Anim. Sci. J.

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The objective of this study was to evaluate the yield, nutrient profile and in vitro digestibility of new BMR mutant lines of sorghum in Indonesia. These mutant lines were GH2.1, GH2.2, GH2.3, GH4.1, GH4.2, GH4.3 and GH4.4. One sorghum mutant line (CTY) and two national sorghum varieties (Super 1 and Bioguma) were also evaluated as controls. In vitro digestibility and rumen fermentation were measured using Ankom Daisy Fermenter and Hohenheim gas test methods, respectively. In vitro measurement consisted of ten treatments with five replications following a completely randomized design. The highest stem sugar content was found in Bioguma (11.22%) and GH4.4 (9.32%) (p<0.05). The Bioguma variety and the GH2.3 mutant line had a higher number of stem segments and fresh forage yield than the Super 1 variety (p<0.05). A greater concentration of crude protein (CP) was observed for the GH.2.1, GH2.2, GH2.3 and GH4.1 lines (p<0.05). The GH2.3 mutant line had the lowest acid detergent lignin (ADL) content (p<0.05), while Bioguma had the highest level of non-fibre carbohydrate (NFC) compounds (p<0.05). The highest relative feed value (RFV) was observed for the GH2.3 line (p<0.05). Furthermore, GH4.2 and GH2.3 had greater in vitro true digestibility (IVTD) (p<0.05) but were not significantly different from Bioguma. Regarding yield characteristics, nutrient composition and in vitro digestibility values, the highest values were found in the Bioguma variety and the GH2.3 mutant line. Except for n-valerate (nC5), significant differences in all rumen fermentation parameters were observed among sorghum cultivars (p<0.05). Regarding the interrelationship between parameters, we found a medium correlation of DMD with the ADL and cellulose content of sorghum forage (R 2 = -0.489 and R 2 = -0.674, respectively). Based on these findings, the GH2.3 BMR mutant line should be further developed as forage sorghum.

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Section: In Vitro Assaymentioning

confidence: 99%

Forage-Yield and Nutrient Quality of New Brown Midrib (BMR) Mutant Lines of Sorghum

Wahyono

Indriatama

Sasongko

et al. 2023

Trop. Anim. Sci. J.

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“…After 48 h of incubation, 20 mL of rumen-buffer was collected from representative samples to measure pH, ammonia (mg%, Conway 1951), and short-chain fatty acids (SCFAs) (mM) [23]. Methane production was estimated on the basis of SCFAs using the following equation [24]: CH 4 (mM) = (0.5 × acetate concentration) + (0.5 × butyrate concentration) -(0.25 × propionate concentration)…”

Section: In Vitro Fermentation Measurementmentioning

confidence: 99%

Use of irradiated chitosan as a matrix for slow-release urea and in vitro fermentation characteristics of slow-release urea supplementation in ruminant rations

Sasongko,

Wahyono,

Astuti

et al. 2024

Vet World

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Background and Aim: Irradiated chitosan can be used as a matrix for slow-release urea (SRU) production. This study aimed to (1) determine the optimal formulation of irradiated chitosan matrix for controlling nitrogen release and (2) evaluate the characteristics of SRU in vitro fermentation based on irradiated chitosan as a feed supplement. Materials and Methods: In the first phase of the investigation, four chitosan-based SRU formulations with varying amounts of acrylamide (3 and 5 g) and gamma irradiation (5 and 10 kGy) were evaluated. Scanning electron microscopy, Fourier transform mid-infrared spectroscopy, and ammonia release characteristics were used to observe morphological, functional group, and ammonia release characteristics. In the second phase of research, the most effective SRU formulation was utilized as a supplement to ruminant rations based on rice straw, sorghum straw, and alfalfa. Gas production, rumen fermentation characteristics, and methane gas production were observed in vitro. Results: On the basis of surface image analysis, the four SRU formulas generate a similar appearance. Compared with untreated urea, the SRU3 formula reduced the percentage of ammonia emission by 12.85%–27.64% after 24 h of incubation (p = 0.05), as determined by the first phase study. SRU3 became the basis for the second testing phase. The addition of SRU3 did not affect the optimal gas production in vitro. SRU3 treatment produced less gas than Optigen® treatment (p = 0.05). With regard to rumen fermentation and digestibility, Optigen® yielded better results than SRU3 (p = 0.05). However, the treatment with SRU3 resulted in reduced methane production compared to that in the control (p = 0.05). Conclusion: Irradiated chitosan as an SRU matrix may control the release of ammonia in the rumen medium. The SRU3 formulation is the most effective. The addition of SRU to rice straw-based rations reduces methane production without affecting in vitro digestibility. Keywords: fermentation characteristics, irradiated chitosan, ruminant, slow-release urea.

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“…Berbagai upaya dilakukan untuk menekan produksi metan di atmosfir telah dilakukan oleh beberapa peneliti. Penelitian yang telah dilakukan dan cukup efektif adalah menggunakan suplemen minyak kelapa (Sitoresmi et al, 2009), virgin coconut oil (VCO) (Sondakh et al, 2015a), minyak kedele dan minyak ikan (Sondakh et al, 2017). Dari beberapa penelitian yang cukup menarik adalah menggunakan minyak kelapa dan minyak sawit.…”

Section: Pendahuluanunclassified

Coconut Oil and Palm Oil Supplementation, as Agents of Methane Reducing in Goat Feed on Meat Quality

Sondakh

Kalele

Ratulangi

2022

JIPT

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This research was conducted to examine feed containing coconut oil and palm oil on the quality of goat meat. Used as many as 20 goats aged + 1 year. Feed was given with a formulation of 60:40% forage and concentrates with a composition according based on needs of goats. This experiment used a CRD with 5 treatments and four replications. The treatments were R0: basal feed; R1 was basal feed added 0.5 mL of coconut oil; R2 was basal feed added 1 mL of coconut oil, R3 was basal feed added 0.5 mL of palm oil and R4 was basal feed added 1 mL of palm oil. The Animals rearing was carried out for 60 days and followed by slaughter of cattle to measure the quality of the meat. The variables measured were pH, WHC, cooking loss, water content, crude protein, crude fat. The data obtained were analyzed by ANOVA. The results shof the meat gave no significant difference (P>0.05). Conclusion that supplementation of coconut oil and palm oil at the level until 1 mL can be used in goat feed.

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Changes in in Vitro Methane Production and Fatty Acid Profiles in Response to Cakalang Fish Oil Supplementation

Cited by 4 publications

References 16 publications

Forage-Yield and Nutrient Quality of New Brown Midrib (BMR) Mutant Lines of Sorghum

Forage-Yield and Nutrient Quality of New Brown Midrib (BMR) Mutant Lines of Sorghum

Use of irradiated chitosan as a matrix for slow-release urea and in vitro fermentation characteristics of slow-release urea supplementation in ruminant rations

Coconut Oil and Palm Oil Supplementation, as Agents of Methane Reducing in Goat Feed on Meat Quality

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