Changes in mechanical and bacterial properties of denture base resin following nanoceria incorporation with and without SBA-15 carriers

Mangal, Utkarsh; Seo, Ji‐Young; Ryu, Jeong-Hyun; Jin, Jie; Wu, Chengzan; Yul, Jung; Lee, Kee‐Joon; Yu, Hyung Seog; Kim, Kwang Mahn; Kwon, Jae‐Sung; Choi, Sung‐Hwan

doi:10.1016/j.jmbbm.2022.105634

Cited by 9 publications

(3 citation statements)

References 42 publications

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“…On the other hand, to assess the pro-inflammatory response RAW 264.7 cells were used at 1 × 10 5 cells per well in 6-well plates. 19 After culturing the cells for 2 days using the extracts containing lipopolysaccharide (LPS; 20 ng ml −1 , Sigma-Aldrich), qPCR assay was performed on RAW 264.7 cells to investigate the expression of the main pro-inflammatory response genes (TNF-α, IL-1β, and IL-6).…”

Section: Methodsmentioning

confidence: 99%

Effect of strontium substitution on functional activity of phosphate-based glass

et al. 2023

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Section: Methodsmentioning

confidence: 99%

Effect of strontium substitution on functional activity of phosphate-based glass

et al. 2023

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“…To evaluate the pro-in ammatory response by qPCR assay, according to previous study [19], cells were seeded in 6-well plates at a concentration of 1 10 5 cells/well. After 24 h, BG and PSr6 powders (1 mg/mL) were added to the cell inserts and cells were cultured in the presence of ionic extracts from the PSr6 and BG powders.…”

Section: Pro-in Ammatory Response Against Bgmentioning

confidence: 99%

Strontium-substituted phosphate-based glass exhibits improved and optimized bioactive in vitro and in vivo responses

Ryu

Lee

Mangal

et al. 2023

Preprint

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Background Phosphate-based glass (PBG) is an attractive bioactive material for promoting the cellular regenerative response. PBG consists of glass network which contains phosphate as the main component and can be substituted into various therapeutic ions. Strontium (Sr) can stimulate osteogenic activity and inhibit the pro-inflammatory response. However, there are limited studies on the characterization and biological performance Sr-substituted PBG (PSr). In this study, PSr was characterized and its of immuno-osteogenic response potential was investigated for substituting Ca with Sr. Considering a priority for cellular activity, we compared the optimized PSr against the benchmark 45S5 bioactive glass (BG) for its effect on cellular bioactive response and regenerative hard tissue. Methods PSr groups were fabricated by a melt-quenching method; subsequently, they were characterized and their biological performance was investigated for the preference of PSr. Considering the clinically used BG as the benchmark, PSr was evaluated based on the cytotoxicity assay, osteogenic activity, pro-inflammatory response, and implantation of calvaria bone defect. Results PSr groups exhibited a glass structure and phosphate network similar to that of PBG. However, the results of ion release analysis showed that PSr6 was stable in accordance with Ca/P, Ca/Sr, and P/Sr ratios. Based on these results, the cellular response of PSr6 was the highest, which increased for the early osteogenic marker and inhibited the inflammatory response. Compared to the clinically used BG as the benchmark, PSr6 promoted osteogenic activity and suppressed inflammatory response. The results of in vivo study indicated that the new bone formation in the PSr6 was similar to that in benchmark BG. However, the limitation of this study was the four weeks of implantation. However, the results of our study suggest that PSr6 is promising for enhancing the immuno-osteogenic response and regenerating hard tissue with the optimized glass structure having Ca/Sr ratio of 2:1. Conclusions The developed PSr6 demonstrated stable physical properties, favorable cytocompatibility, and immune-osteogenic response. Compared with benchmark BG, PSr6 exhibited enhanced immuno-osteogenic response and regeneration of new bone and connective tissues. Therefore, PSr6 is a potential bone grafting material for regenerative hard tissue.

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“…In particular, nanoceria has the anti-oxidant superoxide dismutase (SOD)-mimetic, catalase (CAT)-mimetic, and reactive oxygen species (ROS)-scavenging capability 14 . Specifically, nanoceria (CeO 2 ) play a role in the protective cells against the damage resulting from the ROS which induces oxidative stress by generating the unstable radical oxygen owing to the changing oxidative state between Ce 3+ and Ce 4+ for redox reaction 15 . The reduction of excessive ROS from the damaged cells is necessary to preserve healthy biological functions.…”

Section: Introductionmentioning

confidence: 99%

Cerium doping of 45S5 bioactive glass improves redox potential and cellular bioactivity

Ryu,

Kang,

Choi

et al. 2024

Sci Rep

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Abstract45S5 Bioglass (BG) is composed of a glass network with silicate based on the component and can be doped with various therapeutic ions for the enhancement of hard tissue therapy. Nanoceria (CeO2) has been shown to indicate redox reaction and enhance the biological response. However, few studies focus on the proportion of CeO2-doped and its effect on the cellular bioactivity of CeO2-doped BG (CBG). In this study, we synthesized the CBG series with increasing amounts of doping CeO2 ranging (1 to 12) wt.%. The synthesized CBG series examined the characterization, mineralization capacity, and cellular activity against BG. Our results showed that the CBG series exhibited a glass structure and indicated the redox states between Ce3+ and Ce4+, thus they showed the antioxidant activity by characterization of Ce. The CBG series had a stable glass network structure similar to BG, which showed the preservation of bioactivity by exhibiting mineralization on the surface. In terms of biological response, although the CBG series showed the proliferative activity of pre-osteoblastic cells similar to BG, the CBG series augmented not only the alkaline phosphatase activity but also the osteogenic marker in the mRNA level. As stimulated the osteogenic activity, the CBG series improved the biomineralization. In conclusion, the CBG series might have a potential application for hard tissue therapeutic purposes.

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Changes in mechanical and bacterial properties of denture base resin following nanoceria incorporation with and without SBA-15 carriers

Cited by 9 publications

References 42 publications

Effect of strontium substitution on functional activity of phosphate-based glass

Effect of strontium substitution on functional activity of phosphate-based glass

Strontium-substituted phosphate-based glass exhibits improved and optimized bioactive in vitro and in vivo responses

Cerium doping of 45S5 bioactive glass improves redox potential and cellular bioactivity

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