Changes in membrane lipids and carotenoids during light acclimation in a marine cyanobacterium Synechococcus sp.

Abstract

), was determined in a marine Synechococcus strain. Highperformance liquid chromatography (HPLC) coupled to diode array detector (DAD) or electrospray ionization mass spectrometry (ESI-MS) was used for compound separation and detection. Myxoxanthophyll rose within a time interval of 8 h to 24 h after the onset of exposure to HL. β-carotene content started to decrease after 4 h of the onset of exposure to HL. Zeaxanthin content rose with exposure to HL, but it was only significant after 24 h of exposure. Carotenoid changes are in agreement with a coordinated activity of the enzymes of the myxoxanthophyll biosynthetic pathway, with no rate-limiting intermediate steps. Lipid analysis showed all species with a C18:3/C16:0 composition increased their content, the changes of PG(18:3/16:0) and MGDG(18:3/16:0) being primarily significant. Major lipid changes were also found to occur within 24 h. These changes might suggest reduction and reorganization of the thylakoid membrane structure. Hypotheses are also drawn on the role played by lipid molecule shape and their possible effect in membrane fluidity and protein accommodation.[Montero O, Sánchez-Guijo A, Lubián LM and Martínez-Rodríguez G 2012 Changes in membrane lipids and carotenoids during light acclimation in a marine cyanobacterium Synechococcus sp. J.

Effects of Light Intensity and Nitrogen Starvation on Glycerolipid, Glycerophospholipid, and Carotenoid Composition in Dunaliella tertiolecta Culture

“…Recently, the contribution of the fatty acyl chain composition to photosynthesis has been studied. Montero et al hypothesized that high light leads to an improvement in the photosynthetic activity, and it requires a higher membrane fluidity so that there could be an increase in glycerolipid species with desaturated fatty acyl chains [33]. This report would be in accordance with above result regarding the enhancement in DGDG species with unsaturated C16 fatty acyl chain under high light condition (Table 2).…”

“…MGDG and PG species did not exhibit variations under stress conditions, despite previous findings indicating that they have been found to play an essential role in photosynthesis [33].…”

Engineering of cyanobacteria for the photosynthetic production of limonene from CO2

“…It is generally known that various protective carotenoids accumulate whilst the chlorophyll content decreases under high light conditions (Kilian et al, 2007). In many cyanobacteria, myxoxanthophylls and zeaxanthin often accumulate under high light (Montero et al, 2012). In terms of gene regulation, only two genes (crtB for phytoene synthase and crtP for phytoene desaturase) are reported to be induced by high light in cyanobacteria (Fernandez-Gonzalez et al, 1998).…”

Subchromoplast Sequestration of Carotenoids Affects Regulatory Mechanisms in Tomato Lines Expressing Different Carotenoid Gene Combinations

“…Increased MGDG content (3.6-fold increase; Table 3) was determined in the transgenic line, especially the C16:0, C16:1 cis 9, and C18:1 fatty acid moieties of this complex lipid (see Supplemental Table 2 online). An associated increase of carotenoid and MGDG lipid (C16:0 and C18:3 fatty acids) levels was observed in a marine bacterium (Synechococcus sp) during high-light acclimation (Montero et al, 2012). It seems that the prokaryotic pool of MGDG (C16:0 plus C18:1, from plastids) is positively regulated in parallel with the carotenoid level in the CrtB+I line.…”