Changes in microRNAs expression profile of olive flounder ( Paralichthys olivaceus ) in response to viral hemorrhagic septicemia virus (VHSV) infection

Abdellaoui, Najib; Kim, Min Sun; Choi, Seung Hyuk; Kang, Yue Jai; Kim, Ki Hong

doi:10.1016/j.fsi.2016.03.014

Cited by 48 publications

(28 citation statements)

References 45 publications

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“…Similar to our study, the expression of miR-221 was increased in the liver and spleen of the infected sole, and the in vitro study indicated that both LPS and pIC up-regulated the expression of miR-221 at 6 h post-stimulation [63]. The expression of miR-221-5p was also evaluated in olive flounder in response to VHSV infection, showing increased transcription 24 h post-injection [58]. In olive flounder, miR-221-5p was predicted to target important immune genes (i.e., cd18 and irf5) [58].…”

Section: Mirnas Associated With Both Antiviral and Antibacterial Immusupporting

confidence: 88%

“…A study in human monocytes revealed that miR-146 attenuated TLR and cytokine signaling via a negative feedback loop involving the suppression of IL-1 receptor-associated kinase 1 and TNF receptor-associated factor 6 [57]. In fish, the expression of miR-146 increased in olive flounder (Paralichthys olivaceus) infected with VHSV [58], in Atlantic salmon infected with SAV [19], and in half-smooth tongue sole (Cynoglossus semilaevis) infected with Vibrio anguillarum [59]. In humans, studies have revealed that the expression of miR-221 is up-regulated in several types of cancers and was related to cancer cell proliferation [60][61][62].…”

Section: Mirnas Associated With Both Antiviral and Antibacterial Immumentioning

confidence: 99%

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Dietary Immunostimulant CpG Modulates MicroRNA Biomarkers Associated with Immune Responses in Atlantic Salmon (Salmo salar)

Xue

Woldemariam

Caballero‐Solares

et al. 2019

Cells

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MicroRNAs (miRNAs) are key regulators in fish immune responses. However, no study has previously characterized the impact of polyriboinosinic polyribocytidylic acid (pIC) and formalin-killed typical Aeromonas salmonicida (ASAL) on miRNA expression in Atlantic salmon fed a commercial diet with and without immunostimulant CpG. To this end, first, we performed small RNA deep sequencing and qPCR analyses to identify and confirm pIC- and/or ASAL-responsive miRNAs in the head kidney of salmon fed a control diet. DESeq2 analyses identified 12 and 18 miRNAs differentially expressed in pIC and ASAL groups, respectively, compared to the controls. Fifteen of these miRNAs were studied by qPCR; nine remained significant by qPCR. Five miRNAs (miR-27d-1-2-5p, miR-29b-2-5p, miR-146a-5p, miR-146a-1-2-3p, miR-221-5p) were shown by qPCR to be significantly induced by both pIC and ASAL. Second, the effect of CpG-containing functional feed on miRNA expression was investigated by qPCR. In pre-injection samples, 6 of 15 miRNAs (e.g., miR-181a-5-3p, miR-462a-3p, miR-722-3p) had significantly lower expression in fish fed CpG diet than control diet. In contrast, several miRNAs (e.g., miR-146a-1-2-3p, miR-192a-5p, miR-194a-5p) in the PBS- and ASAL-injected groups had significantly higher expression in CpG-fed fish. Multivariate statistical analyses confirmed that the CpG diet had a greater impact on miRNA expression in ASAL-injected compared with pIC-injected fish. This study identified immune-relevant miRNA biomarkers that will be valuable in the development of diets to combat infectious diseases of salmon.

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Section: Mirnas Associated With Both Antiviral and Antibacterial Immusupporting

confidence: 88%

Section: Mirnas Associated With Both Antiviral and Antibacterial Immumentioning

confidence: 99%

Dietary Immunostimulant CpG Modulates MicroRNA Biomarkers Associated with Immune Responses in Atlantic Salmon (Salmo salar)

Xue

Woldemariam

Caballero‐Solares

et al. 2019

Cells

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“…As identified by deep sequencing at 72 HPS and confirmed by qPCR assay, the expression of miR-2188-3p was up-regulated in response to pIC stimulation at 48 and 72 HPS. As found herein, miR-2188-5p of olive flounder (Paralichthys olivaceus) and Atlantic salmon was responsive to VHSV and SAV infections, respectively Najib et al, 2016). However, the immunoregulatory function of this miRNA remains undetermined.…”

Section: Discussionmentioning

confidence: 53%

“…Small RNA profiling can be applied to improve our understanding of miRNAs involved in antiviral immune responses in teleost fish . Small RNA deep sequencing previously identified virus-/bacteria-responsive miRNAs in teleosts (Najib et al, 2016;Wang et al, 2016). For example, several differentially expressed miRNAs were identified in olive flounder (Paralichthys olivaceus) infected by viral hemorrhagic septicemia virus (VHSV) or megalocytivirus (Najib et al, 2016;Zhang et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…Small RNA deep sequencing previously identified virus-/bacteria-responsive miRNAs in teleosts (Najib et al, 2016;Wang et al, 2016). For example, several differentially expressed miRNAs were identified in olive flounder (Paralichthys olivaceus) infected by viral hemorrhagic septicemia virus (VHSV) or megalocytivirus (Najib et al, 2016;Zhang et al, 2014). A total of thirteen miRNAs were associated with viral or bacterial infections in the studied teleost species, indicating that the immune-related responses of these miRNAs are conserved .…”

Section: Introductionmentioning

confidence: 99%

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Discovery of microRNAs associated with the antiviral immune response of Atlantic cod macrophages

Eslamloo

Inkpen

Rise

et al. 2018

Molecular Immunology

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MicroRNAs (miRNAs) are known to play important immunoregulatory roles in teleosts, although miRNAs involved in the antiviral immune response of Atlantic cod (Gadus morhua) were previously uncharacterised. Using deep sequencing and qPCR, the present study was conducted to identify miRNAs responsive to the viral mimic, polyriboinosinic polyribocytidylic acid (pIC) in Atlantic cod macrophages. Macrophage samples isolated from Atlantic cod (n=3) and treated with pIC or phosphate buffered saline (PBS control) for 24 and 72h were used for miRNA profiling. Following deep sequencing, DESeq2 analyses identified four (miR-731-3p, miR-125b-3-3p, miR-150-3p and miR-462-3p) and two (miR-2188-3p and miR-462-3p) significantly differentially expressed miRNAs at 24 and 72h post-stimulation (HPS), respectively. Sequencing-identified miRNAs were subjected to qPCR validation using a larger number of biological replicates (n=6) exposed to pIC or PBS over time (i.e. 12, 24, 48 and 72 HPS). As in sequencing, miR-731-3p, miR-462-3p and miR-2188-3p showed significant up-regulation by pIC. The sequencing results were not qPCR-validated for miR-125b-3-3p and miR-150-3p as up- and down-regulated miRNAs at 24 HPS, respectively; however, qPCR results showed significant up-regulation in response to pIC stimulation at later time points (i.e. 48 and/or 72 HPS). We also used qPCR to assess the expression of other miRNAs that were previously shown as immune responsive in other vertebrates. qPCR results at 48 and/or 72 HPS revealed that miR-128-3-5p, miR-214-1-5p and miR-451-3p were induced by pIC, whereas miR-30b-3p and miR-199-1-3p expression were repressed in response to pIC. The present study identified ten pIC-stimulated miRNAs, suggesting them as important in antiviral immune responses of Atlantic cod macrophages. Some pIC-responsive miRNAs identified in this study were predicted to target putative immune-related genes of Atlantic cod (e.g. miR-30b-3p targeting herc4), although the regulatory functions of these miRNAs need to be validated by future studies.

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Baculovirus-Encoded MicroRNAs: A Brief Overview and Future Prospects

Tang

Qiu

2018

Curr Microbiol

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MicroRNAs (miRNAs) are a class of non-coding RNAs with ∼22 nucleotides, which are able to regulate various biological processes, including the viral life cycle and host-pathogen interactions. Long primary transcripts (pri-miRNAs) are initially transcribed in nucleus, and subsequently processed by Dicer in cytoplasm to generate mature miRNAs. Baculoviruses consist of large, enveloped, insect-pathogenic viruses with a double-stranded circular DNA genome. Recent studies suggest that baculoviruses encode some miRNAs to manipulate expression regulation of host genes, whereas host modulate viral gene expression via miRNAs to limit viral infection. In the review, we will focus on the biogenesis and functions of miRNAs and the interactions between baculoviruses, insect, and miRNAs. It will be helpful to delve into the related mechanisms of BmNPV-encoded miRNAs that contribute to infection and pathogenesis.

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Changes in microRNAs expression profile of olive flounder ( Paralichthys olivaceus ) in response to viral hemorrhagic septicemia virus (VHSV) infection

Cited by 48 publications

References 45 publications

Dietary Immunostimulant CpG Modulates MicroRNA Biomarkers Associated with Immune Responses in Atlantic Salmon (Salmo salar)

Dietary Immunostimulant CpG Modulates MicroRNA Biomarkers Associated with Immune Responses in Atlantic Salmon (Salmo salar)

Discovery of microRNAs associated with the antiviral immune response of Atlantic cod macrophages

Baculovirus-Encoded MicroRNAs: A Brief Overview and Future Prospects

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