Changes in milk and plasma concentrations of progesterone in cows after treatment to induce superovulation and their relationships with number of ovulations and of embryos collected

Saumande, J.; Tamboura, D.; Chupín, D.

doi:10.1016/0093-691x(85)90147-5

Cited by 97 publications

(36 citation statements)

References 19 publications

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“…In all females, blood samples were taken by jugular venipuncture and plasma was obtained after centrifugation at 3500 3 g for 25 min and stored at 2208C until hormone concentrations were measured. Progesterone concentration in plasma was measured by RIA (Saumande et al, 1985). Sensitivity was 0.1 ng/ml.…”

Section: Measurementsmentioning

confidence: 99%

Stimulation of reproductive activity in anovulatory Alpine goats exposed to bucks treated only with artificially long days

Delgadillo

Vélez

2010

Animal

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Two experiments were conducted in a subtropical latitude to determine the response of Alpine male goats to a treatment with artificially long days (experiment 1), and the response of anovulatory lactating Alpine does exposed to males treated only with artificially long days (experiment 2). In experiment 1, one group of males was kept under natural photoperiod ( n 5 4) while another was exposed to 2.5 months of artificially long days (16 h of light/day) from 1 December ( n 5 4). Plasma testosterone concentrations were determined weekly. Intensity of odor of males was determined every 2 weeks. Sexual behavior of bucks was observed during 3 days about 90 days after the end of the long day treatment. A treatment-by-time interaction was detected for testosterone secretion ( P , 0.001). In control males, low plasma concentrations of testosterone were observed from March to June. In contrast, in long-day treated males, high levels of testosterone were observed from March to June ( P , 0.05). A treatment-by-time interaction was detected for the intensity of male odor ( P , 0.01). The male odor was stronger in long-day treated bucks than in untreated ones from March to June ( P , 0.05). The number of ano-genital sniffing, nudging and flehmen was greater in long-day treated males than in untreated ones when exposed to anestrous does ( P , 0.05). In experiment 2, one group of males was left under natural photoperiod variations ( n 5 5) and the other ( n 5 5) was submitted to the same photoperiodic treatment described in experiment 1. On 3 May, three untreated and three long-day treated males were put in contact with anestrous Alpine does left under natural photoperiod. Fertility was higher in does exposed to light-treated males (36/45, 80%) than those in contact with untreated ones (3/45, 7%; P , 0.05). Prolificacy was similar ( P . 0.05) in does exposed to treated (1.8 6 0.1) and untreated males (1.7 6 0.3). These results indicate that the sexual activity of Alpine male goats raised in subtropical latitudes can be induced using only artificially long days and that such males are effective in stimulating reproductive activity in anovulatory females in late spring.Keywords: seasonal anestrous, photoperiod, male effect, sexual behavior, ovulation ImplicationsThese results demonstrated that, in lactating Alpine female goats raised in subtropical latitudes, sexual activity can be induced during the anestrous season by exposure to males rendered sexually active by a long-day treatment. These results have an important practical application because they show that in subtropical latitudes the photoperiodic treatment of females is not necessary to stimulate their reproductive activity by the male effect, in contrast to that reported for the same breed under temperate latitudes.

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Section: Measurementsmentioning

confidence: 99%

Stimulation of reproductive activity in anovulatory Alpine goats exposed to bucks treated only with artificially long days

Delgadillo

Vélez

2010

Animal

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“…The concentrations of E2 and P4 in the culture medium of GC from large follicles were measured after 144 h of culture by radioimmunoassay protocols previously described (Saumande 1981, Saumande et al 1985, Saumande 1991) and adapted to directly measure steroids in cell culture media. The limit of detection of E2 was 1·5 pg/tube (7·5 pg/well) and the intra-and interassay coefficients of variation were less than 7% and 9% respectively.…”

Section: Estradiol-17 and Progesterone Radioimmunoassaysmentioning

confidence: 99%

Laminin-alpha6beta1 integrin interaction enhances survival and proliferation and modulates steroidogenesis of ovine granulosa cells

Bellego¹,

Pisselet²,

Huet³

et al. 2002

Journal of Endocrinology

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This study aimed to determine the physiological role of laminin (LN) and its receptor, 6 1 integrin, in controlling the functions of granulosa cells (GC) during follicular development in sheep ovary. Immunohistochemistry experiments showed the presence of increasing levels of LN (P<0·0001), and high levels of mature 6 1 integrin in GC layers of healthy antral follicles during the follicular and the preovulatory phases of the estrous cycle. In vitro, the addition of a function-blocking antibody raised against 6 subunit (anti-6 IgG) to the medium of ovine GC cultured on LN impaired cell spreading (P<0·0001), decreased the proliferation rate (P<0·05) and increased the apoptosis rate (P<0·05). Furthermore, addition of anti-6 IgG enhanced estradiol (E2) secretion by GC in the presence or absence of follicle-stimulating hormone (FSH), luteinizing hormone or insulin-like growth factor-I in culture medium (P<0·0001), and inhibited progesterone (P4) secretion in basal conditions or in the presence of low (0·5 ng/ml) FSH concentrations only (P<0·0001). The anti-6 IgG effect was specific to an interaction of LN with 6 1 integrin since it was ineffective on GC cultured on heat-denatured LN, RGD (arginine-glycine-aspartic acid) peptides and non-coated substratum. Hence, this study established that 6 1 integrin 1) was expressed in GC of antral follicles, 2) mediated the actions of LN on survival, proliferation and steroidogenesis of GC, and 3) was able to dramatically modulate P4 and E2 secretion by GC in vitro. It is suggested that during the follicular and the preovulatory phases of the estrous cycle, the increasing levels of LN in GC of large antral follicles might support their final development to ovulation.

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“…Plasma progesterone levels were determined by radio-immuno-assay, as described previously [23]. The limit of detection was 0.05 ng .…”

Section: Lh and Progesterone Determinationsmentioning

confidence: 99%

Relationship between peri-oestrus progesterone levels and time of ovulation by echography in pigs and influence of the interval between ovulation and artificial insemination (AI) on litter size

Terqui¹,

Guillouet²,

Maurel³

et al. 2000

Reprod. Nutr. Dev.

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-Two methods for the determination of ovulation were compared to one ultrasonography performed 5 times a day. Time of ovulation by echography was 40 ± 5.8 h (mean ± SD) after the onset of oestrus. Preovulatory LH rise (two blood samples per day) began near the onset of oestrus but, in our conditions, this parameter could not be used to predict ovulation. The basal level of progesterone (two blood samples per day) was determined with a non-linear model, the timing when progesterone rose more than one SD (0.3 ng . mL -1 ) coincided with the timing of ovulation determined by echography (R 2 = 0.98). This method was efficient and was used in a field trial to measure the consequences of the variability of the interval between AI and ovulation on litter size. The interval between AI and ovulation had an effect on litter size; litter size decreased by one piglet when this interval increased by 10 h. pig / progesterone / echography / ovulation / prolificacy Résumé -Chez la truie, relation entre la progestéronémie et le moment d'ovulation déterminé par échographie et incidence sur la taille de la portée de l'intervalle entre l'ovulation et l'insé-mination artificielle (IA). Deux méthodes de détermination de l'ovulation sont comparées à celle fondée sur 5 échographies par jour. Le moment d'ovulation par échographie est de 40 ± 5,8 h (moyenne ± sd) après le début de l'oestrus. Les concentrations plasmatiques de LH (mesurées 2 fois par jour) augmentent dès le début de l'oestrus mais dans nos conditions, ce paramètre ne permet pas de prédire l'ovulation. Le moment où la progestérone augmente de 1 SD (0,3 ng . mL -1 ) au-dessus du niveau de base coïncide avec le moment d'ovulation déterminé par échographie (R 2 = 0,98). Cette méthode, avec 2 prélèvements par jour, est donc fiable et a été utilisée en élevages pour étudier les conséquences de Reprod. Nutr. Dev. 40 (2000) 393-404 393

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Changes in milk and plasma concentrations of progesterone in cows after treatment to induce superovulation and their relationships with number of ovulations and of embryos collected

Cited by 97 publications

References 19 publications

Stimulation of reproductive activity in anovulatory Alpine goats exposed to bucks treated only with artificially long days

Stimulation of reproductive activity in anovulatory Alpine goats exposed to bucks treated only with artificially long days

Laminin-alpha6beta1 integrin interaction enhances survival and proliferation and modulates steroidogenesis of ovine granulosa cells

Relationship between peri-oestrus progesterone levels and time of ovulation by echography in pigs and influence of the interval between ovulation and artificial insemination (AI) on litter size

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