Changes in morphology and miRNAs expression in small intestines of Shaoxing ducks in response to high temperature

Tian, Yong; Li, Gongqi; Bu, Xiaoqing; Shen, Junda; Zhang, Tao; Chen, Li; Zeng, Tao; Du, Xue; Liu, Lizhi

doi:10.1007/s11033-019-04827-2

Cited by 7 publications

(7 citation statements)

References 59 publications

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“…The reduced abundance of Lactococcus , Geobacillus , and Pseudomonas might be associated with the decrease of muscularis thickness and the height of intestinal microvillus, as well as the upregulation of relative expression of IL-1β, IL-10, and IL-17F ( Miao et al., 2018 ). High ambient temperature causes deleterious impacts on intestinal morphology and mucosal barrier integrity in poultry ( Farag and Alagawany, 2018 ), and this has been confirmed by our previous work ( Tian et al., 2019 ). Thus, the variation in the abundance of Enterococcus , Weissella , Lactococcus , Geobacillus , and Pseudomonas observed in the present study may be associated with intestinal injury induced by heat treatment.…”

Section: Discussionsupporting

confidence: 82%

“…Enterococcus is characterized by its prebiotic properties in the intestine, which has been reported to have beneficial effects on enhancing the intestinal barrier function ( Pedicord et al., 2016 , Huang et al., 2019 ). Previously, we found that high temperatures caused damage to the intestinal mucosa of ducks ( Tian et al., 2019 ). Therefore, it can be inferred that adverse impacts on the integrity of the intestinal mucosal barrier induced by high ambient temperature may be related to the decrease in the abundance of Enterococcus .…”

Section: Discussionmentioning

confidence: 98%

“…To improve land utilization and feed conversion rate and reduce environmental pollution, Shaoxing ducks were normally transformed from flat culture to cage rearing at the age of 80 D. In production, we found that a certain number of ducks died early in the culture mode alteration, especially in the first 2 wk, and the high environmental temperature has greatly exacerbated this negative result. Here, we focused on the effect of high temperature on duck gut microbiome, and our previous report has shown that high temperature induced a certain degree of damage to the intestinal mucosa of ducks ( Tian et al. 2019 ).…”

Section: Introductionmentioning

confidence: 99%

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High-temperature exposure alters the community structure and functional features of the intestinal microbiota in Shaoxing ducks (Anas platyrhynchos)

Tian

Chen

et al. 2020

Poultry Science

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The gut microbiome is a complex ecosystem that contributes to host nutrition and health. However, our current knowledge of the relationship between ambient temperature and gut microbiota of poultry is still limited. The objective of the present study was to characterize the intestinal microbiota of ducks exposed to high ambient temperature. Sixty 60-day-old Shaoxing ducks were allocated to control and heat-treated groups. The ducks in the control group were kept at 25°C, and the ducks in the heat treatment group were raised at 30–40°C, which simulated the temperature change of day and night in summer. After 15 D, the intestinal contents of the duodenum, jejunum, and ileum were obtained from 6 ducks of each group. Genomic DNA was extracted and amplified based on the V4–V5 hypervariable region of 16S rRNA. The results showed that Firmicutes was the dominant bacterial phylum with the highest abundance in the contents of the small intestine of ducks, and the relative abundance of the phylum Firmicutes in all 3 intestinal segments was increased by high temperature. At the genus level, Lactobacillus was found to be the most dominant bacterial genus across 3 gut segments, and its abundance was increased in ducks under heat treatment. Compared with the corresponding intestine segment of control ducks, a total of 36 genera in the duodenum, 19 genera in the jejunum, and 6 genera in the ileum of heat-treated ducks were found to be significantly different in the abundance (linear discriminant analysis score >3.0, P < 0.05). Functional prediction of gut microbiota revealed that high temperature caused changes in the abundance of metabolism and transcription-related pathways. It is noteworthy that most of the altered pathways are related to metabolism. In conclusion, high temperature induced remarkable taxonomic changes in the gut microbiome of ducks, which might be related to the negative effects of high temperature in ducks. Our present study provided an important theoretical ground for high-temperature intervention.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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High-temperature exposure alters the community structure and functional features of the intestinal microbiota in Shaoxing ducks (Anas platyrhynchos)

Tian

Chen

et al. 2020

Poultry Science

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“…The authors compared the DE genes and the putative targets of the altered miRNAs and identified 82 candidate target genes with potential roles in cellular responses to stress and lipid metabolism (180). The response to thermal stress at the morphological and molecular levels were investigated in the intestine of ducks exposed to high (30-40 • C) or control temperatures (25 • C) (181). By comparing the same tissues under different conditions, they identified 16, 18, and 15 miRNAs that were dysregulated between the duodenum, jejunum, and ileum, respectively, of control and heat-treated ducks.…”

Section: Environmental Stressmentioning

confidence: 99%

MicroRNAs as Biomarkers for Animal Health and Welfare in Livestock

et al. 2020

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MicroRNAs (miRNAs) are small and highly conserved non-coding RNA molecules that orchestrate a wide range of biological processes through the post-transcriptional regulation of gene expression. An intriguing aspect in identifying these molecules as biomarkers is derived from their role in cell-to-cell communication, their active secretion from cells into the extracellular environment, their high stability in body fluids, and their ease of collection. All these features confer on miRNAs the potential to become a non-invasive tool to score animal welfare. There is growing interest in the importance of miRNAs as biomarkers for assessing the welfare of livestock during metabolic, environmental, and management stress, particularly in ruminants, pigs, and poultry. This review provides an overview of the current knowledge regarding the potential use of tissue and/or circulating miRNAs as biomarkers for the assessment of the health and welfare status in these livestock species.

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“…Studies have shown that oxidative stress acts as a stimulus for stress-induced intestinal mucosal injury [5]. The intestinal tract of ducks possesses a large surface area, allows the exchange of nutrients into the systemic circulation, and prevents penetration of toxic compounds [6][7][8], and maintenance of the integrity of the intestinal mucosa is important [9,10].…”

Section: Introductionmentioning

confidence: 99%

Analysis of miRNAs and their target genes associated with mucosal damage caused by transport stress in the mallard duck intestine

et al. 2020

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Bowel health is an important factor for duck rearing that has been linked to feed uptake and growth and death rates. Because the regulatory networks associated with acute stressmediated injury in the duck gastrointestinal tract have not clearly elucidated, we aimed to explore potential miRNA-mRNA pairs and their regulatory roles in oxidative stress injury caused by transport stress. Here, 1-day-old mallard ducklings from the same breeder flock were collected and transported for 8 h, whereas the control group was not being transported. Various parameters reflecting oxidative stress and the tissue appearance of the intestine were assessed. The data showed that the plasma T-AOC and SOD concentrations were decreased in the transported ducklings. The intestine of the transported ducklings also displayed significant damage. High-throughput sequencing of the intestine revealed 44 differentially expressed miRNAs and 75 differentially expressed genes, which constituted 344 miRNA-mRNA pairs. KEGG pathway analysis revealed that the metabolic, FoxO signaling, influenza A and TGF-β signaling pathways were mainly involved in the mechanism underlying the induction of intestinal damage induced by simulated transport stress in ducks. A miRNA-mRNA pair, miR-217-5p/CHRDL1, was selected to validate the miRNA-mRNA negative relationship, and the results showed that miR-217-5p could influence CHRDL1 expression. This study provides new useful information for future research on the regulatory network associated with mucosal damage in the duck intestine.

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Changes in morphology and miRNAs expression in small intestines of Shaoxing ducks in response to high temperature

Cited by 7 publications

References 59 publications

High-temperature exposure alters the community structure and functional features of the intestinal microbiota in Shaoxing ducks (Anas platyrhynchos)

High-temperature exposure alters the community structure and functional features of the intestinal microbiota in Shaoxing ducks (Anas platyrhynchos)

MicroRNAs as Biomarkers for Animal Health and Welfare in Livestock

Analysis of miRNAs and their target genes associated with mucosal damage caused by transport stress in the mallard duck intestine

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