Changes in mouse Leydig cells ultrastructure and testosterone secretion after diethylcarbamazine administration

Saraiva, Karina Lidianne Alcântara; Silva, Valdemiro Amaro Da; Torres, Diogo; Donato, Mariana Aragão Matos; Peres, Newton G.; Souza, José Roberto Botêlho de; Peixoto, Christina Alves

doi:10.1016/j.micron.2007.06.003

Cited by 9 publications

(4 citation statements)

References 34 publications

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“…In relation to this, some studies on the effect of opium drugs on testosterone serum level have shown a significant decrease in experimental groups compared with the control group (30). In a survey on testosterone secretion in mice, it was shown that using diethylcarbamazine daily at the dose of 200 mg/kg of body weight can cause a significant decrease in testosterone secretion (31). In this survey, LH serum level in experimental groups increased compared with the control group.…”

Section: Tablementioning

confidence: 76%

Evaluation of histopathologic and histomorphometric changes of testicular tissue and gonadotropin levels following consumption of methylphenidate in male mice

Fazelipour¹,

Tootian²,

Saremi³

et al. 2014

Turk J Med Sci

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Section: Tablementioning

confidence: 76%

Evaluation of histopathologic and histomorphometric changes of testicular tissue and gonadotropin levels following consumption of methylphenidate in male mice

Fazelipour¹,

Tootian²,

Saremi³

et al. 2014

Turk J Med Sci

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“…The size and number of Leydig cells per gram of testis are also known to exhibit remarkable variation among different mammalian species (Christensen and Fawcett, 1966;Hess and França, 2007). A number of studies comparing different mammalian species have demonstrated that the amount of testosterone produced is strongly correlated with the bulk of the smooth endoplasmic reticulum and mitochondria in Leydig cells (Haider, 2004;Saraiva et al, 2008). Although Leydig cell size in peccaries may differ from the values observed for domestic and wild boars (França et al, 2005;Almeida et al, 2006), the number per gram of testis in these 3 related species is quite high and situated on the upper level for the mammalian species investigated thus far (Hess and França, 2007).…”

Section: Discussionmentioning

confidence: 97%

Spermatogenic Cycle Length and Sperm Production in a Feral Pig Species (Collared Peccary, Tayassu tajacu)

Costa

Leal

Silva

et al. 2010

Journal of Andrology

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Although the collared peccary (Tayassu tajacu) is found throughout the Americas, with a high potential for domestication and commercial exploitation, there are few data on the reproductive biology of this mammalian species. The aim of the present study was to investigate testis structure, spermatogenic cycle length, Sertoli cell efficiency, and spermatogenic efficiency. Twelve adult peccaries were used for biometrical, histological, and stereological analyses; 3 of these peccaries received intratesticular injections of 3 H-thymidine for the determination of the duration of spermatogenesis. Testis weight and gonadosomatic index were 23.7 6 1.8 g and 0.2% 6 0.1%, respectively. Seminiferous tubule volume density was 77.4% 6 1.7%. Leydig cells occupied 12.8% 6 1.8% of the testis parenchyma and presented a peculiar cytoarchitecture in the periphery of the seminiferous tubule lobes. The premeiotic, meiotic, and postmeiotic stage frequencies were very similar to those found for wild and domestic boars. The spermatogenic cycle and entire spermatogenic process (based on 4.5 cycles) lasted approximately 12.3 6 0.2 and 55.1 6 0.7 days, respectively. Daily sperm production per gram of testis in the collared peccary was approximately 23.4 6 2 6 10 6 , which is similar to that of domestic and wild boars. The knowledge generated in the present study could be used in reproduction and animal improvement programs and provides important information that may be used for comparative reproductive biology with previously investigated mammalian species.

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“…As seen in representative electron micrographs (Figure 1), large whorls of stacked RER membranes were observed in midgut epithelial cells from unfed (sugar only) mosquitoes, with some RER whorls containing >50 stacked membranes. The whorls appeared to consist entirely of tightly packed RER membranes, with no evidence of a core lipid droplet as seen in other organisms [25]. Quantitative measurements of whorl size in 20 randomly chosen EM fields revealed that the RER whorls ranged in size from ∼5–35 µm 2 in unfed mosquitoes (Figure 2).…”

Section: Resultsmentioning

confidence: 87%

“…For example, treatment of mouse Leydig cells with the piperazine derivative diethylcarbamazine citrate (DEC), led to the appearance of large lipid droplets, degenerative mitochondria, and giant smooth ER whorls in some cells [25]. Since DEC has been shown to disrupt ER and golgi vesicle transport processes, it could explain the formation of ER whorls.…”

Section: Discussionmentioning

confidence: 99%

Alpha-COPI Coatomer Protein Is Required for Rough Endoplasmic Reticulum Whorl Formation in Mosquito Midgut Epithelial Cells

et al. 2011

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BackgroundOne of the early events in midgut epithelial cells of Aedes aegypti mosquitoes is the dynamic reorganization of rough endoplasmic reticulum (RER) whorl structures coincident with the onset of blood meal digestion. Based on our previous studies showing that feeding on an amino acid meal induces TOR signaling in Ae. aegypti, we used proteomics and RNAi to functionally identify midgut epithelial cell proteins that contribute to RER whorl formation.Methodology/Principal FindingsAdult female Ae. aegypti mosquitoes were maintained on sugar alone (unfed), or fed an amino acid meal, and then midgut epithelial cells were analyzed by electron microscopy and protein biochemistry. The size and number of RER whorls in midgut epithelial cells were found to decrease significantly after feeding, and several KDEL-containing proteins were shown to have altered expression levels. LC-MS/MS mass spectrometry was used to analyze midgut microsomal proteins isolated from unfed and amino acid fed mosquitoes, and of the 127 proteins identified, 8 were chosen as candidate whorl forming proteins. Three candidate proteins were COPI coatomer subunits (alpha, beta, beta'), all of which appeared to be present at higher levels in microsomal fractions from unfed mosquitoes. Using RNAi to knockdown alpha-COPI expression, electron microscopy revealed that both the size and number of RER whorls were dramatically reduced in unfed mosquitoes, and moreover, that extended regions of swollen RER were prevalent in fed mosquitoes. Lastly, while a deficiency in alpha-COPI had no effect on early trypsin protein synthesis or secretion 3 hr post blood meal (PBM), expression of late phase proteases at 24 hr PBM was completely blocked.Conclusionsalpha-COPI was found to be required for the formation of RER whorls in midgut epithelial cells of unfed Aa. aegypti mosquitoes, as well as for the expression of late phase midgut proteases.

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Changes in mouse Leydig cells ultrastructure and testosterone secretion after diethylcarbamazine administration

Cited by 9 publications

References 34 publications

Evaluation of histopathologic and histomorphometric changes of testicular tissue and gonadotropin levels following consumption of methylphenidate in male mice

Evaluation of histopathologic and histomorphometric changes of testicular tissue and gonadotropin levels following consumption of methylphenidate in male mice

Spermatogenic Cycle Length and Sperm Production in a Feral Pig Species (Collared Peccary, Tayassu tajacu)

Alpha-COPI Coatomer Protein Is Required for Rough Endoplasmic Reticulum Whorl Formation in Mosquito Midgut Epithelial Cells

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