Changes in osteoblast and osteoclast cell count after moringa oleifera leaf extract administration during orthodontic tooth movement

Abstract

Objective: The aim of this study was to analyze the effect of moringa leaf extract administration at 5%, 10%, and 20% concentration toward osteoblast and osteoclast cell count in tension area of Cavia cobaya tooth movement. Material and Methods: This study was an experimental research with post test only control group design conducted on Cavia Cobaya. The samples were divided into four groups, namely control group (given orthodontic forces), P1 group (given orthodontic forces and moringa oleifera extract at 5% concentration), P2 group (given orthodontic forces and moringa oleifera extract at 10% concentration) and P3 group (given orthodontic forces and moringa oleifera extract at 20% concentration). The samples were sacrificed in day 15 and the tissue were collected for histological test to examine osteoblast and osteoclast cell count. Results: The Anova test showed a significant difference of osteoblast and osteoclast cell count among groups (p<0.05). Conclusion: Moringa leaves extract administration at 20% concentration for seven days shows higher number of osteoblast and lower number of osteoclast compared to control group, 5% and 10% extract group.

Post-tooth extraction induction effect of Moringa oleifera leaf extract and demineralized freeze-dried bovine bone xenograft treatment on alveolar bone trabecula area

“…18 Flavonoids can also regulate cell function by stimulating TGFβ production, which induces osteoblast proliferation and migration. 19 Kaempferol and quercetin in Moringa can also directly inhibit RANKL and TNFα activity by inhibiting the inflammatory pathway. It is expected that there will be a reduction in macrophage infiltration, followed by a decrease in proinflammatory cytokines (TNFα, IL1β, and IL6), which will then reduce RANKL production and decrease alveolar bone resorption.…”