Changes in peripheral blood inflammatory factors (TNF-α and IL-6) and intestinal flora in AIDS and HIV-positive individuals

Lu, Jing; Ma, Saisai; Zhang, Weiying; Duan, Jianping

doi:10.1631/jzus.b1900075

Cited by 24 publications

(15 citation statements)

References 37 publications

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“…In another study, the results indicated that deficiencies of IL-10, IL-10Rα and IL-10Rβ may result in dysbiosis of the caecal microbiota in mice, characterized by expanded populations of opportunistic bacteria of the families Enterococcaceae and Enterobacteriaceae ( 67 ). In patients with immune deficiency syndrome, the amount of Enterococcus faecalis was positively correlated with the contents of TNF-α and IL-6 and the CD4 + T-lymphocyte count ( 68 ). In patients with liver cirrhosis, the level of Enterococcus has also been indicated to be positively correlated with AST levels (P<0.05) and Bifidobacterium was negatively correlated with AST (P<0.05) ( 69 ).…”

Section: Discussionmentioning

confidence: 99%

Distinctive gut microbial dysbiosis between chronic alcoholic fatty liver disease and metabolic‑associated fatty liver disease in mice

et al. 2021

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The gut microbiota, which may affect normal physiological and biochemical functions, has an important role in the development of human liver diseases. The aim of the present study was to investigate differences in the gut microbiota between chronic alcoholic fatty liver disease (AFLD) and metabolic-associated fatty liver disease (MAFLD). AFLD was induced by chronic alcohol administration and MAFLD was induced by a Western-style diet in C57BL/6 mice. After 8 weeks, the levels of plasma alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), total cholesterol (TC), lipopolysaccharide (LPS), tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-1β and IL-10 were assessed and H&E staining of mouse liver tissue was performed. High-throughput sequencing of 16S ribosomal DNA from the intestinal contents was used to analyze the different effects of AFLD and MAFLD on the gut microbiota. Differences in the gut microbiota composition were assessed by the t-test. The results revealed increases in LPS, ALT, AST, TG, IL-1β and TNF-α in the AFLD group. Compared with those in the MAFLD control group, the MAFLD group exhibited increased plasma ALT, TG, TC, IL-6, IL-1β and TNF-α levels and decreased plasma IL-10 levels. In addition, the α-and β-diversities revealed that the AFLD and MAFLD groups exhibited obvious changes in the gut structure (with an increase in abundance in the AFLD group and a decrease in abundance in the MAFLD group). In comparison to the AFLD control group, Enterococcaceae were the most abundant bacteria at the family level and Enterococcus and Streptococcus were the most abundant bacteria at the genus level in the AFLD group. However, in the MAFLD group, Lachnospiraceae was the most abundant at the family level, with increases in Erysipelatoclostridium, Gordonibacter and Streptococcus at the genus level and a decrease in the genus Bifidobacterium. In conclusion, the present study confirmed that the AFLD and MAFLD groups harbored differences in the gut microbiota. The marked differences in the gut microbiota at the family and genus levels may contribute to the development process of FLD.

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Section: Discussionmentioning

confidence: 99%

Distinctive gut microbial dysbiosis between chronic alcoholic fatty liver disease and metabolic‑associated fatty liver disease in mice

et al. 2021

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“…Bifidobacteria are commensal microorganisms of the human GI tract and are thought to play pivotal roles in maintaining human health. Moreover, studies have shown that Bifidobacteria can significantly inhibit TNF-α and IL-6 levels ( Lu et al., 2019 ; Nie et al., 2020 ). In the present study, we found that the abundance of Bifidobacterium in patients with primary HSP was higher than in patients with recurrent HSP but the difference was not statistically significant.…”

Section: Discussionmentioning

confidence: 99%

Differences in Manifestations and Gut Microbiota Composition Between Patients With Different Henoch-Schonlein Purpura Phenotypes

Zhang

Xia

Nie

et al. 2021

Front. Cell. Infect. Microbiol.

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BackgroundGut microbiota plays an important role in the pathogenesis of immune-mediated diseases. However, the complex pathogenesis of Henoch-Schonlein Purpura (HSP) remains elusive. This study aimed to characterize the gut microbiota in HSP patients and explore the potential association between gut microbiota composition and phenotypic changes in HSP.Methods16SrRNA gene sequencing and bioinformatic analyses were performed using total DNA extracted from the fecal microbiota of 34 children with HSP, including 18 primary cases, 16 recurrent cases, and 23 healthy children.ResultsThe diversity indexes showed significant differences in the microbial community among the primary HSP groups, the recurrent HSP group and healthy controls. The abundance of Escherichia-Shigella in the recurrent HSP group was significantly higher than that in the primary HSP group, and the constructed ROC curve had an AUC value of 0.750. According to the Spearman correlation analysis, the abundance of Bacteroides was positively associated with the serum IgG level in children with HSP, while the abundance of Lachnoclostridium was negatively correlated with the complement component 3 (C3). The diversity indexes of gut microbiota in the HSP group with abdominal symptoms were higher than those in the HSP group without GI involvement, and also higher than those in the healthy control group. In the HSP group with GI involvement, the abundance of Faecalibacterium was decreased, while the abundance of Streptococcus and Fusobacteria was increased, compared to the HSP group without GI involvement.ConclusionsThe gut microbiota of children with HSP was different from that of healthy children. The genus Escherichia-Shigella has a diagnostic value for HSP recurrence. Bacteroides and Lachnoclostridium may affect IgG and complement C3 levels in children with HSP. Abdominal symptoms in HSP children were related to gut microbiota (Streptococcus and butyric acid-producing bacteria).

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“…Intra-articular injections of IL-6 reproduced OA-like cartilage lesions in mice, and IL-6 knockout (KO) mice develop less severe posttraumatic OA lesions [50]. Lu J, et al investigated that correlation between the amount of intestinal ora and the number of CD4 + T lymphocytes and the levels of TNF-α and IL-6 [51]. In our study, we found that antibiotics reduce the in ammatory response throughout the body after inhibiting the intestinal ora.…”

Section: Discussionmentioning

confidence: 99%

Potential efficacy of gut microbiome dysbiosis on alleviation the progress of osteoarthritis in mice

Guan¹,

Jia²,

Zhang³

et al. 2020

Preprint

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ObjectiveTo evaluate the relationship between the gut microbiota dysbiosis and progression of osteoarthritis (OA)DesignWe used a mice OA model (8 weeks) of destabilization of medial meniscus and the gut microbiome dysbiosis induced by antibiotic treatment(ABT) with ampicillin and neomycin for 8 weeks. The severity of OA was evaluated by micro-CT, X-ray and histology of osteoarthritis Research International (OARSI) score. Microbiome analysis by 16 s DNA qPCR was performed on the feces samples and the serum IL-6, TNF-α, osteocalcin, estrogen, calcium and magnesium ion were measured in enzyme-linked immunosorbent assay(ELISA).ResultCompare to the normal mice, the bone volume over total volume(BV/TV)(P < 0.01 for both subgroup analysis) and the osteoarthritis Research International (OARSI) score (P < 0.01 for both subgroup analysis) and in subchondral bone and was decreased significant in ABT treatment of female and male group. ABT decreased the osteophyte score in female mice with standard normal group(P < 0.05) and also declined osteophyte maturity score in both female(P < 0.01) and male(P < 0.05)mice. The level of IL-6(P < 0.01 for both subgroup analysis), TNF-α(P < 0.01 for both subgroup) and calcium(P < 0.01 for both subgroup analysis) were decreased significant in ABT group than control group while the magnesium(P < 0.01 for both subgroup analysis) and estrogen(P < 0.01 for both subgroup analysis) were increased significant in ABT female group than that in the control female group. Pearson’s correlation analyses demonstrated that subchondral bone BV/TV(r = -0.6145, P = 0.0001) and OARSI score in medial tibial plateau(r = -0.5407, P = 0.0007) are negatively correlated with the Firmicutes : Bacteroidetes ratio.ConclusionGut microbiome dysbiosis have a potential efficacy on alleviate the progress of OA.

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Changes in peripheral blood inflammatory factors (TNF-α and IL-6) and intestinal flora in AIDS and HIV-positive individuals

Cited by 24 publications

References 37 publications

Distinctive gut microbial dysbiosis between chronic alcoholic fatty liver disease and metabolic‑associated fatty liver disease in mice

Distinctive gut microbial dysbiosis between chronic alcoholic fatty liver disease and metabolic‑associated fatty liver disease in mice

Differences in Manifestations and Gut Microbiota Composition Between Patients With Different Henoch-Schonlein Purpura Phenotypes

Potential efficacy of gut microbiome dysbiosis on alleviation the progress of osteoarthritis in mice

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