Changes in phospholipid composition of human aorta with age

Rouser, George; Solomon, Robert D.

doi:10.1007/bf02532637

Cited by 54 publications

(12 citation statements)

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“…The purity of the lipids was checked by thin-layer chromatography (TLC), and only one spot was detected on the TLC plate (Kieselgel 60, Merck). Lipids were quantified with the method of Rouser et al (2). 2-(4,4-Difluoro-5,7- dimethyl-4-bora-3a,4a-diaza-s-indacene-3-dodecanoyl)-1-hexadecanoyl-sn-glycero-3-phosphocholine (Bodipy-C 12 -PC) was purchased from Molecular Probes (Eugene, OR).…”

Section: Materials Sn-(3-myristoyl-2-hydroxy)glycerol-1-phospho-sn-1′mentioning

confidence: 99%

“…The difference in this tilt angle of the lipids accounts for the difference in the thickness of the membranes. The total system areas of the headgroups of 1,1′-and 3,1′-DMBMPs, which contain 128 molecules, were 71.20 and 64.97 nm 2 , respectively (Figure 10c,d).…”

Section: Differential Scanning Calorimetry (Dsc)mentioning

confidence: 99%

“…BMPs comprise less than 1% of the total phospholipids in most mammalian cells (2,3). However, the content is increased to 15% in late endosomes (4).…”

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confidence: 99%

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Differential Membrane Packing of Stereoisomers of Bis(monoacylglycero)phosphate

et al. 2006

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Bis(monoacylglycero)phosphate (BMP) reveals an unusual sn-1,sn-1′ stereoconfiguration of glycerophosphate. We synthesized sn- (3-myristoyl-2-hydroxy)glycerol-1-phospho-sn-1′-(3′-myristoyl-2′-hydroxy)glycerol (1,1′-DMBMP) and characterized the thermotropic phase behavior and membrane structure, in comparison with those of the corresponding sn-3:sn-1′ stereoisomer (3,1′-DMBMP), by means of differential scanning calorimetry (DSC), small-and wide-angle X-ray scattering (SAXS and WAXS, respectively), pressure-area (π-A) isotherms, epifluorescence microscopy of monolayers, and molecular dynamics (MD) simulations. In DSC, these lipids exhibited weakly energetic broad peaks with an onset temperature of 9°C for 1,1′-DMBMP and 18°C for 3,1′-DMBMP. In addition, a highly cooperative, strongly energetic transition peak was observed at ∼40°C for 1,1′-DMBMP and ∼42°C for 3,1′-DMBMP. These results are supported by the observation that 1,1′-DMBMP exhibited a larger phase transition pressure (π c ) than 3,1′-DMBMP. Small-and wide-angle X-ray scattering measurements identified these small and large energetic transitions as a quasi-crystalline (L c1 )-quasi-crystalline with different tilt angle (L c2 ) phase transition and an L c2 -L R main phase transition, respectively. X-ray measurements also revealed that these DMBMPs undergo an unbinding at the main phase transition temperature. The MD simulations estimated stronger hydrogen bonding formation in the 3,1′-DMBMP membrane than in 1,1′-DMBMP, supporting the experimental data.Bis(monoacylglycero)phosphate, also known as lysobisphosphatidic acid (LBPA), was first described in pig and rabbit pulmonary alveolar macrophages by Body and Gray (1). BMPs comprise less than 1% of the total phospholipids in most mammalian cells (2, 3). However, the content is increased to 15% in late endosomes (4). In the specific internal membrane domains of this organelle, this lipid comprises more than 70% of the total phospholipids (5).Because of the high level of accumulation in late endosomes, BMP is assumed to be important for the structural and functional integrity of the organelle. Indeed, a series of experiments has demonstrated that agents that perturb BMP induce alterations in the sorting and trafficking of proteins and/or the receptor in late endosomes accompanied by structural changes of the late edonsome organelle (4, 6) along with abnormal accumulation of cholesterol (7). Moreover, BMP is found to be essential for the activator-stimulated hydrolysis of ganglioside G M1 (8) and G M2 (9) and for the hydrolysis of ceramide by acid ceramidase (10).BMP has unique structural characteristics with various fatty acid compositions depending on the source. Whereas all other mammalian phospholipids display a sn-3-glycerophosphate stereoconfiguration, BMP possesses an sn-1:sn-1′ structure and one acyl group attached to each of the glycerol moieties (11-13). An sn-3:sn-1′ isomer has also been reported for BMP in BHK cells (14). However, the sn-3:sn-1′ isomer in mammalian cells is postulated to be...

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Section: Materials Sn-(3-myristoyl-2-hydroxy)glycerol-1-phospho-sn-1′mentioning

confidence: 99%

Section: Differential Scanning Calorimetry (Dsc)mentioning

confidence: 99%

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Differential Membrane Packing of Stereoisomers of Bis(monoacylglycero)phosphate

et al. 2006

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“…Phospholipid and cholesterol pool sizes were determined as described (18,19). FA and TG pool sizes were measured by gas chromatography using C19 as internal standard as described (20).…”

Section: Enzymatic Assays and Other Determinationsmentioning

confidence: 99%

FATP1 channels exogenous FA into 1,2,3-triacyl-sn-glycerol and down-regulates sphingomyelin and cholesterol metabolism in growing 293 cells

Hatch

Smith

et al. 2002

Journal of Lipid Research

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“…Lipid phosphates were measured according to the method of Rouser et al (32). Briefly, 20% of the lipid sample was dried under nitrogen and oxidized with 70% perchloric acid on a heating block at 160°C for 45 min.…”

Section: Experimental Designmentioning

confidence: 99%

Modulation of Ceramide Content and Lack of Apoptosis in the Chronically Hypoxic Neonatal Rat Heart

Bitar

Sabban

et al. 2002

Pediatr Res

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To assess the effect of chronic hypoxia on cardiomyocyte apoptosis, we used an animal model that mimics cyanotic heart disease. Rats were placed in a hypoxic environment at birth, and oxygen levels were maintained at 10% in an air-tight Plexiglas chamber. Controls remained in room air. Animals were killed, and the hearts were harvested at 1 and 4 wk. Significant polycythemia developed in the hypoxic rats at 1 and 4 wk. Right ventricular mass in the hypoxic rats was 192% and 278% that of controls, and hypoxic left ventricular mass was 140% and 178% that of the controls at 1 and 4 wk, respectively. The increase in cardiac mass was paralleled by only mild hypertrophy (10 to 20%). Contrary to previous reports showing increased apoptosis in response to hypoxia in cultured cardiomyocytes, there was no difference in the number of apoptotic cardiomyocytes between the chronically hypoxic rats and controls, as assayed by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling and Hoechst staining. We then examined the role of the sphingolipid ceramide because of its reported role in the stress response, growth suppression, and apoptosis. We found that the right ventricular ceramide content was significantly decreased in the hypoxic rats to 73% of control levels at the age of 4 wk. We suggest that the decrease in the ceramide content in the hypoxic right ventricular rat heart may be an adaptive response to chronic hypoxia and pulmonary hypertension. Lower ceramide levels may help suppress apoptosis and allow compensatory right ventricular cardiomyocyte proliferation. Apoptosis, or programmed cell death, is a mechanism of regulated cell death that plays a major role during development and homeostasis, and in many disease states. Apoptosis in cardiomyocytes has been demonstrated after injury caused by ischemia and reperfusion (1, 2), myocardial infarction (3, 4), cardiac aging (5), ventricular pacing (6), heart failure, and coronary embolization (7,8).Conflicting data regarding apoptotic changes, in response to hypoxia, have been described (9 -12). The failing myocardium is subject to regional ischemia and hypoxia, an abnormality that can trigger cardiomyocyte apoptosis (13). Long et al. (14) reported that cultured neonatal rat cardiomyocytes exposed to in vitro hypoxia for 48 h exhibited apoptotic changes. However, Bishopric et al. (15) found that induction of apoptosis of cardiac myocytes by hypoxia required acidosis, and that chronic hypoxia alone did not cause apoptosis of cardiomyocytes in culture. Hypoxia can induce cellular adaptive responses, such as c-Jun expression, that overcome apoptosis signals to minimize injury or damage (16). The in vivo effect of chronic hypoxia on the neonatal heart has not been investigated, and differentiating the effect of hypoxia and ischemia from that of hypoxia alone on apoptosis in the neonatal heart is important.Although no universal definition of apoptosis currently exists, morphologic and biochemical characteristics of apoptosis have been described that distinguis...

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Changes in phospholipid composition of human aorta with age

Cited by 54 publications

References 3 publications

Differential Membrane Packing of Stereoisomers of Bis(monoacylglycero)phosphate

Differential Membrane Packing of Stereoisomers of Bis(monoacylglycero)phosphate

FATP1 channels exogenous FA into 1,2,3-triacyl-sn-glycerol and down-regulates sphingomyelin and cholesterol metabolism in growing 293 cells

Modulation of Ceramide Content and Lack of Apoptosis in the Chronically Hypoxic Neonatal Rat Heart

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