Changes in physicochemical properties of chitin at developmental stages (larvae, pupa and adult) of Vespa crabro (wasp)

Kaya, Murat; Sofi, Karwan; Sargın, İdris; Mujtaba, Muhammad

doi:10.1016/j.carbpol.2016.03.010

Cited by 78 publications

(56 citation statements)

References 32 publications

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“…Similar structures were found in the present work for CHIVV. Morphologies that were identified using SEM and classificated as (1) microporous smooth surface, (2) fish scale shaped nano fibrous surface, (3) adherent fibres with nano pores, and (4) separated fibres with nano pores were reported previously for Vespa crabro [31]. Similar structures were found in the present work for CHI VV .…”

Section: Scanning Electron Microscopy (Sem)supporting

confidence: 86%

“…Scanning electron microscopy is a highly suitable method for observing the structure of polymers, in general [41][42][43], and anatomical variation in CHI organization in insect cuticles, in particular [44]. Morphologies that were identified using SEM and classificated as (1) microporous smooth surface, (2) fish scale shaped nano fibrous surface, (3) adherent fibres with nano pores, and (4) separated fibres with nano pores were reported previously for Vespa crabro [31]. Similar structures were found in the present work for CHIVV.…”

Section: Scanning Electron Microscopy (Sem)mentioning

confidence: 69%

“…Based on the above results, CHI VV is close to pure and full acetylated. In previous researches using related insects, the % of C, H, and N content in CHI were recorded as 46.62, 6.42, and 6.85 for Vespa crabro; 46.01, 6.71, and 6.34 for Vespa orientalis [28], and 45.5, 6.3, and 6.49 for Vespa crabro, respectively [31].…”

Section: Elemental Analysis (Ea) Andmentioning

confidence: 94%

“…Two new sources as fungi and insect species have been recently researched as an alternative origin for these functional materials in order to expand the chitin-chitosan based source and obtain a better material and more consistent quality [3,7,[27][28][29][30][31]. The insect and fungal biopolymers have many features that can make them more advantageous than those biopolymers from seafood waste origin [32], due to potential advantages in terms of homogenous polymer length, high degree of deacetylation and solubility over the current marine source [7].…”

Section: Introductionmentioning

confidence: 99%

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Extraction and Physicochemical Characterization of Chitin Derived from the Asian Hornet, Vespa velutina Lepeletier 1836 (Hym.: Vespidae)

et al. 2020

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Fifteen years ago, at least one multimated female yellow-legged Asian hornet (Vespa velutina Lepeletier 1836) arrived in France, which gave rise to a pan-European invasion. In this study, the isolation and characterization of chitin (CHI) that was obtained from Vespa velutina (CHIVV) is described. In addition, an easy procedure is carried out to capture the raw insect, selectively and with high rates of success. The chitin contents of dry VV was observed to be 11.7%. Fourier transform infrared spectroscopy (FTIR), solid-state NMR (ssNMR), elemental analysis (EA), scanning electron microscopy (SEM), and thermogravimetric analysis (TG) characterized the physicochemical properties of CHIVV. The obtained CHIVV is close to pure (43.47% C, 6.94% H, and 6.85% N), and full acetylated with a value of 95.44%. Additionally, lifetime and kinetic parameters such as activation E and the frequency factor A using model-free and model-fitting methods, were determined. For CHIVV the solid state mechanism that follows the thermodegradation is of type F2 (random nucleation around two nuclei). The invasive Asian hornet is a promising alternative source of CHI, based on certain factors, such as the current and probable continued abundance of the quantity and quality of the product obtained.

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Section: Scanning Electron Microscopy (Sem)supporting

confidence: 86%

Section: Scanning Electron Microscopy (Sem)mentioning

confidence: 69%

Section: Elemental Analysis (Ea) Andmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

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Extraction and Physicochemical Characterization of Chitin Derived from the Asian Hornet, Vespa velutina Lepeletier 1836 (Hym.: Vespidae)

et al. 2020

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“…The TGA curve of I-ChBD-CadA was different from that of chitin but shared similar tendency with ChBD-CadA. For chitin, a weight loss of ∼5% was observed in the range 30-110 • C due to the evaporation of water (Kaya et al, 2016). While in the range 280-400 • C, most of the weight loss has been accomplished, leading to a carbonaceous material residue (Qiao et al, 2015).…”

Section: Characterization Of Immobilized Enzyme On Chitinmentioning

confidence: 88%

Cadaverine Production From L-Lysine With Chitin-Binding Protein-Mediated Lysine Decarboxylase Immobilization

Zhou

Zhang

Wei

et al. 2020

Front. Bioeng. Biotechnol.

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Lysine decarboxylase (CadA) can directly convert L-lysine to cadaverine, which is an important platform chemical that can be used to produce polyamides. However, the non-recyclable and the poor pH tolerance of pure CadA hampered its practical application. Herein, a one-step purification and immobilization procedure of CadA was established to investigate the cadaverine production from L-lysine. Renewable biomass chitin was used as a carrier for lysine decarboxylase (CadA) immobilization via fusion of a chitin-binding domain (ChBD). Scanning electron microscopy, laser scanning confocal microscopy, fourier transform infrared spectra, elemental analysis, and thermal gravimetric analysis proved that the fusion protein ChBD-CadA can be adsorbed on chitin effectively. Furthermore, the fusion protein (ChBD-CadA) existed better pH stability compared to wild CadA, and kept over 73% of the highest activity at pH 8.0. Meanwhile, the ChBD-CadA showed high specificity toward chitin and reached 93% immobilization yield within 10 min under the optimum conditions. The immobilized ChBD-CadA (I-ChBD-CadA) could efficiently converted L-lysine at 200.0 g/L to cadaverine at 135.6 g/L in a batch conversion within 120 min, achieving a 97% molar yield of the substrate L-lysine. In addition, the I-ChBD-CadA was able to be reused under a high concentration of L-lysine and retained over 57% of its original activity after four cycles of use without acid addition to maintain pH. These results demonstrate that immobilization of CadA using chitin-binding domain has the potential in cadaverine production on an industrial scale.

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Sources of Chitin and Chitosan and their Isolation

Bastiaens

Soetemans

Dhondt

et al. 2019

Chitin and Chitosan

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Changes in physicochemical properties of chitin at developmental stages (larvae, pupa and adult) of Vespa crabro (wasp)

Cited by 78 publications

References 32 publications

Extraction and Physicochemical Characterization of Chitin Derived from the Asian Hornet, Vespa velutina Lepeletier 1836 (Hym.: Vespidae)

Extraction and Physicochemical Characterization of Chitin Derived from the Asian Hornet, Vespa velutina Lepeletier 1836 (Hym.: Vespidae)

Cadaverine Production From L-Lysine With Chitin-Binding Protein-Mediated Lysine Decarboxylase Immobilization

Sources of Chitin and Chitosan and their Isolation

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