Changes in Plasma Lipid and Antioxidant Activity in Rats as a Result of Naringin and Red Grapefruit Supplementation

Abstract

The aim of this investigation was to compare the influence of naringin versus red grapefruit juice on plasma lipid levels and plasma antioxidant activity in rats fed cholesterol-containing and cholesterol-free diets. The antioxidant activity of a correlated quantity of red grapefruit juice was higher than that of naringin. Forty-two male Wistar rats were randomly divided into six groups of 7 named control, naringin, grapefruit, Chol, Chol/naringin, and Chol/grapefruit. The rats of the control group were fed basal diet (BD) and 1-2 mL of distilled water. To the BD of the other five groups were added 0.46-0.92 mg of naringin dissolved in 1-2 mL of distilled water (naringin), 1-2 mL of red grapefruit juice (grapefruit), 1% of nonoxidized cholesterol (NOC) and 1-2 mL of distilled water (Chol), 1% of NOC and 0.46-0.92 mg of naringin in 1-2 mL of water (Chol/naringin), and 1% of NOC and 1-2 mL of red grapefruit juice (Chol/grapefruit). After 30 days of different feeding, it was found that diets supplemented with red grapefruit juice and to a lesser degree with naringin improved the plasma lipid levels mainly in rats fed cholesterol and increased the plasma antioxidant activity. In conclusion, naringin is a powerful plasma lipid lowering and plasma antioxidant activity increasing flavonone. However, fresh red grapefruit is preferable than naringin: it more effectively influences plasma lipid levels and plasma antioxidant activity and, therefore, could be used as a valuable supplement for disease-preventing diets.

Grapefruit Juice Suppresses Azoxymethane-induced Colon Aberrant Crypt Formation and Induces Antioxidant Capacity in Mice

“…Moreover, the inhibition of DNA oxidation by GJ on mutagens and teratogens has also been determined in vitro and in vivo; in this respect, oxidized guanine residues in hydrogen peroxide-treated human lymphocytes were lowered by the juice as shown by adding the enzyme formamidopyrimidine-DNA-glycosylase to the comet assay, similarly, it was reported a reduction in the amount of the adduct 8-hydroxy-2'-deoxyguanosine in the serum of cadmium-treated fetuses (Razo-Aguilera et al, 2011;Argüelles et al, 2012). In fact, the antioxidant potential of GJ, as well as of various constituents has been clearly established in a number of tests, although only two of them has been carried out ex vivo (Alvarez-González et al, 2004;Gorinstein et al, 2005;Sengupta et al, 2006;Ademosun and Oboh, 2012). Also, a relationship between antioxidation and colon crypt inhibition has been documented in studies measuring lipoperoxidation, as well as enzymatic and non-enzymatic compounds (Nirmala and Ramanathan 2011a;2011b;Khan and Sultana, 2011).…”

“…On the other hand, a number of reports have established the strong antioxidant potential of GJ, as well as of several of its components (Alvarez-Gonzalez et al, 2004;Gorinstein et al, 2005). Moreover, this effect has been suggested to be, at least partially, the subjacent explanation for the observed antigenotoxicity of the juice.…”

Yoghurt kedelai hitam (black soyghurt) dapat menurunkan kadar LDL tikus hiperkolesterolemia

“…Kelompok pertama diberi perlakuan pakan tinggi kolesterol dan black soyghurt 2 mL, kelompok kedua diberi perlakuan pakan tinggi kolesterol dan black soyghurt 3 mL, kelompok ketiga diberi perlakuan pakan tinggi kolesterol dan black soyghurt 4 mL dan perlakuan pakan tinggi kolesterol saja pada kelompok kontrol. Oleh karena itu, pada 20 Seluruh subjek diadaptasi terlebih dahulu selama 7 hari dan diberi pakan standar rodentia dan minum air ad libithum. Selanjutnya, subjek diberi pakan tinggi kolesterol dan hanya pada kelompok perlakuan diberikan black soyghurt dengan dosis yang berbeda.…”

“…Mekanisme penurunan kadar lipid pada hewan coba akibat konsumsi fl avonoid disebabkan oleh beberapa faktor. Faktor tersebut antara lain penghambatan penyerapan kolesterol dan peningkatan ekskresi empedu (20). Selain itu, fl avonoid juga mampu menghambat aktivitas enzim 3-hidroksi-3-metilglutaril CoA yang berperan dalam penghambatan sintesis kolesterol serta enzim acylCoA: Cholesteryl aciltransferase yang berperan dalam penurunan esterifikasi kolesterol pada usus dan hati.…”

Quality Changes of Fresh Vegetable and Fruit Juice by Various Juicers

“…따라서 채소 및 과일주스의 섭취에 따른 효능 및 효과 의 검증에 관한 연구도 무척 활발하다 [1]. 예를 들어 오렌지 (Orange)와 자몽 (red grapefruit)을 식이 했을 경우 동물의 혈 장 지질과 항산화 활성이 증가한다는 보고 이후 다양한 연구 가 진행되고 있다 [2,3]. 또한 과일주스에 포함된 성분 가운데 pycnogenol이 농축된 주스 (농도 0.5 g/L)를 이용하여 대장암 세포주 (Caco-2)의 증식의 억제 효과가 있다는 연구가 발표 되기도 하였다 [4].…”