Changes in polyamine content, arginine and ornithine decarboxylases and transglutaminase activities during light/dark phases (of initial differentiation) in maize calluses and their chloroplasts

Bernet, Esmaragda; Claparols, I.; Dondini, Luca; Santos, M. Teresa; Serafini-Fracassini, Donatella; Torné, J. M.

doi:10.1016/s0981-9428(99)00104-7

Cited by 46 publications

(46 citation statements)

References 28 publications

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“…3d). Similar results were observed by Bernet et al (1999) with maize callus, which demonstrated that with cells cultured in a maintenance medium, the changes were not significant in light and dark treatments, except for free Put levels which increased in the dark period of culture. In plants, PAs often occur as free molecular bases, but they can also be associated with small molecules such as phenolic acids (conjugated forms), by the formation of an amide linkage (reviewed in Bouchereau et al, 1999).…”

Section: Resultssupporting

confidence: 83%

“…Wisconsin-38 (Floh and Handro, 1985a) has been carried out, until now no work was undertaken to study the PA pattern in these tissue. The effects of light and dark culture conditions on endogenous levels of PAs contents (µg.g PAs have been demonstrated by several authors (Bernet et al, 1999;Arena et al, 2003). In this work, a similar pattern of total PAs (free + conjugated) in diploid and haploid callus maintained in the light was observed (Fig.…”

Section: Resultsmentioning

confidence: 85%

“…The results observed in this work suggested the possible effects of a dark condition on endogenous conjugated Spd levels. However, until now few papers have been published showing the effects of the dark on conjugated PA contents (Bernet et al, 1999). These authors showed an increase in …”

Section: Resultsmentioning

confidence: 99%

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Polyamine patterns in haploid and diploid tobacco tissues and in vitro cultures

Carone

Santa–Catarina

Silveira

et al. 2010

Braz. arch. biol. technol.

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Section: Resultssupporting

confidence: 83%

Section: Resultsmentioning

confidence: 85%

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Polyamine patterns in haploid and diploid tobacco tissues and in vitro cultures

Carone

Santa–Catarina

Silveira

et al. 2010

Braz. arch. biol. technol.

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“…cytoskeleton, cell walls, nuclei, chloroplasts and vacuoles. Subcellular compartmentation of plant TGases has been observed in Helianthus leaves and maize calli; 27,50,51 in some compartments TGase could play a specific role. As suggested by the present data, the highest production of bis-derivatives, especially bis-PU, occurs in the phases of development and anthesis (stages 3 ± 5) when the corolla changes shape in order to favour the pollination process.…”

Section: Corolla Of Undetached¯owersmentioning

confidence: 97%

“…Chloroplast TGases (ChlTGases) are different from those of the cytoplasm and are active in chloroplasts of both higher and lower plants. 20,27,51,57,58 Having as substrates the antenna complexes and Rubisco, 26,27 they are possibly involved in photosynthesis. The decrease in total TGase activity during petal senescence could thus be partly related to the breakdown of chloroplasts.…”

Section: Corolla Of Undetached¯owersmentioning

confidence: 99%

Transglutaminase activity during senescence and programmed cell death in the corolla of tobacco (Nicotiana tabacum) flowers

et al. 2002

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Corolla life span of undetached flowers of Nicotiana tabacum was divided into stages from the closed corolla (stage 1) through anthesis (stage 5) to death (stage 9). Senescence began around stage 6 in the proximal part, concomitantly with DNA laddering. Nuclear blebbing, DNA laddering, cell wall modification, decline in protein, water, pigment content and membrane integrity were observed during senescence and PCD. Transglutaminase activity was measured as mono-and bis-derivatives of putrescine (mono-PU; bis-PU) and bis-derivatives of spermidine (bis-SD). Bis-derivatives decreased with the progression of senescence, while mono-PU increased during early senescence; derivatives were present in different amounts in the proximal and distal parts of the corolla. In excised flowers, exogenous spermine delayed senescence and PCD, and caused an increase in free and acid-soluble conjugated PA levels. Bis-PU was the most abundant PA-derivative before DNA laddering stage; thereafter, bis-PU generally decreased and mono-PU became the most abundant derivative.

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Activity of maize transglutaminase overexpressed in escherichia coli inclusion bodies: An alternative to protein refolding

Carvajal

Gibert

Campos

et al. 2011

Biotechnology Progress

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Transglutaminases (TGases) catalyze protein post-translational modification by ε-(γ-glutamyl) links and covalent polyamine conjugation. In plants, this enzyme is poorly characterized and only the maize plastidial TGase gene (tgz) has been cloned. The tgz gene (Patent WWO03102128) had been subcloned and overexpressed in Escherichia coli cells, and the recombinant protein (TGZp) was present mainly in inclusion bodies (IB) fraction. In this work, after overexpression of TGZ15p and SDS-PAGE IB fraction analysis, bands about 65 and 56 kDa were obtained. Western blot, alkylation and MALDI-TOF/TOF analyses indicated that the 56 kDa band corresponded to a truncated sequence from the native TGZ15p (expected MW 65 kDa), by elimination of a chloroplast signal peptide fragment during expression processing. So that large-scale protein production and protein crystallization can be applied, we characterized the TGZ15p enzyme activity in the IB protein fraction, with and without refolding. Results indicate that it presented the biochemical characteristics of other described TGases, showing a certain plant-substrate preference. Solubilization of the IB fraction with Triton X-100 as nondenaturing detergent yielded active TGZ without the need for refolding, giving activity values comparable to those of the refolded protein, indicating that this is a valuable, faster way to obtain TGZ active protein.

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Changes in polyamine content, arginine and ornithine decarboxylases and transglutaminase activities during light/dark phases (of initial differentiation) in maize calluses and their chloroplasts

Cited by 46 publications

References 28 publications

Polyamine patterns in haploid and diploid tobacco tissues and in vitro cultures

Polyamine patterns in haploid and diploid tobacco tissues and in vitro cultures

Transglutaminase activity during senescence and programmed cell death in the corolla of tobacco (Nicotiana tabacum) flowers

Activity of maize transglutaminase overexpressed in escherichia coli inclusion bodies: An alternative to protein refolding

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