Changes in Quality of Native and Frozenthawed Semen in Relation to Two Collections Performed in a 24-hour Interval and Adition of Clarified Egg Yolk to Extender

Abstract

The aim of the study was to evaluate the effect of repeated semen collection and the substitution of normal egg yolk with clarified egg yolk to commercially produced semen extender on qualitative parameters of frozen-thawed canine semen. Two semen collections were scheduled in a 24-hour interval and in each of six dogs, three 1st and three 2nd collections were performed. The frozen-thawed sperm samples were prepared either with clarified or normal egg yolk and motility and viability were evaluated. The effect of the sequence of semen collection was demonstrated by significant differences in motility and also in viability of sperms both in native and frozen-thawed ejaculate. The percentage of viable sperms was significantly higher in samples from the 2nd compared to the 1st collection. This trend was the same also in motility except in native ejaculate. The addition of clarified egg yolk was beneficial for higher survival of sperms immediately after thawing and also after 30 min of incubation, compared to samples with normal egg yolk. Sperm motility evaluated after thawing was higher in samples with clarified egg yolk, without an apparent connection with semen collection sequence. The decrease of values of the qualitative parameters of sperms observed in the period of 30 min of incubation was significantly slowed down when clarified egg yolk was used. This was especially obvious in samples from the 2nd collection.

Effect of two freezing extenders on characteristic of fresh and frozen-thawed semen in endangered Old Kladruber stallions - A pilot study

“…It is meaningful to also evaluate CASA kinematic parameters when the effect of different types of extenders on sperm quality needs to be evaluated objectively (Folkova et al 2016). In the ejaculates from the Old Kladruber stallions, the VAP, VCL, and VSL significantly differed between chosen extenders immediately after dilution.…”

Effect of repeated semen ejaculation on sperm quality and selected biochemical markers of canine semen

“…In the present study, total sperm counts were highest in the ejaculates acquired on day 1 and decreased gradually on successive days of the experiment. Folková et al (2016) also observed a decrease in sperm concentration in ejaculates obtained at 24-h intervals from dogs after three months of sexual rest. The cited authors attributed these findings to the depletion of extragonadal sperm reserves in the epididymis and vasa deferentia (Root-Kustritz 2007).…”

“…Research has shown that ejaculates collected from dogs after a long period of sexual abstinence can contain a large number of dead spermatozoa, and spermatozoa with abnormal morphology and decreased motility (Feldman and Nelson 2004). For example, Folková et al (2016) noted that the percentage of motile and viable spermatozoa was lower in ejaculates obtained after three months of sexual abstinence than in ejaculates sampled after another 24 hours. However, Taha et al (1983) found that that ejaculates collected after six weeks of sexual abstinence were characterized by normal quality parameters.…”