Diverse soil phosphorus (P)-leaching phenomena induced by environmental disturbance have gained increasing attention. Two kinds of typical organic materials, biochar and biogas slurry, (BS) are widely utilized to amend agricultural soil, but there is little research that gives insight into their co-effects on soil P-leaching and corresponding mechanisms. Herein, a total of six treatments (viz., control, 2% (w/w) biochar, low ratio BS with or without 2% (w/w) biochar, high ratio BS with or without 2% (w/w) biochar) were conducted to investigate the P-leaching and fraction transformation mechanisms. The column experiment results showed that compared to control, sole BS application or biochar both can slightly enhance the soil-P loss by 134.8% and 39.8%. High ratios of BS induced higher P loss than the low ratios of BS by 125.1%. In comparison with the sole BS treatment, combined BS and biochar application increase P loss but result in less soil leaching of basic cations. The incubation experiment results showed that the enhanced P-leaching in combined BS and biochar treatment is probably attributable to the enhanced soil pH, decreased DPS, soil P adsorption capacity, and transformation of moderately labile Fe–P into labile P. This research helps in understanding the abiotic process of biochar and BS in promoting soil P-leaching and soil-P management using biochar and biogas slurry.